##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632928.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	332341
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.847647446448075	33.0	33.0	33.0	27.0	33.0
2	30.598280079797558	33.0	33.0	33.0	27.0	33.0
3	31.031949112507935	33.0	33.0	33.0	27.0	33.0
4	31.21494188198266	33.0	33.0	33.0	27.0	33.0
5	31.327840982605217	33.0	33.0	33.0	27.0	33.0
6	33.902497735759354	37.0	33.0	37.0	27.0	37.0
7	34.15518097375888	37.0	33.0	37.0	27.0	37.0
8	34.32220520489497	37.0	37.0	37.0	27.0	37.0
9	34.41632840967561	37.0	37.0	37.0	27.0	37.0
10	34.488161256059286	37.0	37.0	37.0	27.0	37.0
11	34.43510430551753	37.0	37.0	37.0	27.0	37.0
12	34.473940922125166	37.0	37.0	37.0	27.0	37.0
13	34.44523546598223	37.0	37.0	37.0	27.0	37.0
14	34.456025588176	37.0	37.0	37.0	27.0	37.0
15	34.457861052352854	37.0	37.0	37.0	27.0	37.0
16	34.437430229794096	37.0	37.0	37.0	27.0	37.0
17	34.42841539262384	37.0	37.0	37.0	27.0	37.0
18	34.41618397970759	37.0	37.0	37.0	27.0	37.0
19	34.43653957832467	37.0	37.0	37.0	27.0	37.0
20	34.46921986754568	37.0	37.0	37.0	27.0	37.0
21	34.46537141068962	37.0	37.0	37.0	27.0	37.0
22	34.44760652462381	37.0	37.0	37.0	27.0	37.0
23	34.43566698060125	37.0	37.0	37.0	27.0	37.0
24	34.42205746507352	37.0	37.0	37.0	27.0	37.0
25	34.422755543252265	37.0	37.0	37.0	27.0	37.0
26	34.36452619448097	37.0	37.0	37.0	27.0	37.0
27	34.30839108024589	37.0	37.0	37.0	27.0	37.0
28	34.256958966844294	37.0	37.0	37.0	27.0	37.0
29	34.296668181175356	37.0	37.0	37.0	27.0	37.0
30	34.238219178494376	37.0	37.0	37.0	27.0	37.0
31	34.22202195937305	37.0	37.0	37.0	27.0	37.0
32	34.267072073563	37.0	37.0	37.0	27.0	37.0
33	34.23409389753296	37.0	37.0	37.0	27.0	37.0
34	34.13017051763099	37.0	37.0	37.0	27.0	37.0
35	34.13470802579278	37.0	37.0	37.0	27.0	37.0
36	34.08030005325855	37.0	37.0	37.0	27.0	37.0
37	34.1355354891512	37.0	37.0	37.0	27.0	37.0
38	34.00447732900846	37.0	33.0	37.0	27.0	37.0
39	34.059183188351724	37.0	33.0	37.0	27.0	37.0
40	34.10127248819737	37.0	37.0	37.0	27.0	37.0
41	34.126027182923565	37.0	37.0	37.0	27.0	37.0
42	34.004684947087476	37.0	33.0	37.0	27.0	37.0
43	33.74896266184431	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	7.0
16	13.0
17	17.0
18	11.0
19	24.0
20	89.0
21	197.0
22	548.0
23	1108.0
24	2119.0
25	3496.0
26	5161.0
27	7182.0
28	9429.0
29	12069.0
30	14676.0
31	18154.0
32	22196.0
33	27785.0
34	37597.0
35	61583.0
36	108878.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.95071026445729	20.693504563084304	12.81424801634466	25.54153715611375
2	16.484574578520256	23.685311171357128	36.247709430976016	23.5824048191466
3	18.40398867428334	27.028263139365894	30.056478135409115	24.511270050941654
4	13.33870933769833	18.44190154088722	38.215266849410696	30.00412227200375
5	14.650614880499246	36.74478923756021	34.98394721084669	13.620648671093846
6	30.008936604270914	39.93759421798695	16.886872218594757	13.16659695914738
7	26.67561330079647	32.215405261463374	23.46595815743468	17.64302328030547
8	24.583184139182347	35.97600055364821	20.664016777947953	18.77679852922149
9	26.27120939035509	14.859436542587282	20.05169389271862	38.81766017433901
10	14.904570907591902	28.36875378000307	34.04003719071677	22.686638121688265
11	34.575932551204936	23.35011328725616	23.858627132974867	18.215327028564037
12	22.81993494633524	25.694091309829364	30.21685557905886	21.26911816477654
13	28.00286452769896	22.16037142573441	26.06238772826705	23.774376318299577
14	22.022862060353674	20.76722402592518	27.64660393992917	29.56330997379198
15	24.10114912093302	28.499342542749766	24.423408487065995	22.976099849251224
16	23.119025338432515	28.21800500088764	25.137133245672366	23.52583641500748
17	22.582829082177643	27.46245573071032	26.32717600296082	23.62753918415122
18	23.111502944265077	26.954543676525017	27.674888141998732	22.25906523721118
19	24.67646182685856	26.276023722622245	27.14681607144469	21.900698379074505
20	24.002455309456252	26.44121549853915	27.35262877586575	22.203700416138844
21	23.03026108725676	27.124850680475777	27.31291053466169	22.53197769760577
22	23.00438405132078	26.956649946891897	27.123647097408988	22.915318904378335
23	22.967373872016992	26.694268838331713	27.59484986805721	22.743507421594085
24	23.223135273709833	27.11431932864137	26.39156769703407	23.270977700614733
25	23.257136495346646	26.745120222903584	27.59454897229051	22.40319430945926
26	23.393141381893898	27.02585597323231	27.175702065047645	22.405300579826143
27	23.203877944641196	26.916630809921134	26.777917861473604	23.101573383964062
28	22.758853105695657	26.813423561943907	27.582212245855914	22.845511086504526
29	23.81349276797025	26.755952470504692	26.803494001642893	22.62706075988217
30	23.38471630042637	26.593167860721366	27.278909313024872	22.74320652582739
31	23.305881609551633	26.73729693296945	27.045414198067647	22.911407259411266
32	22.36919308782245	26.723455727701367	27.43507421594086	23.47227696853533
33	22.524154407671638	26.811317291577026	27.831353940681407	22.833174360069926
34	23.23998543664489	26.290466719423723	27.45944677304335	23.010101070888034
35	23.089537553296164	27.00148341612982	27.13056770004303	22.77841133053099
36	23.634459786785257	26.841707764013467	26.77761696570691	22.74621548349436
37	23.054934540125956	26.380434553666266	27.142904426477628	23.421726479730157
38	23.21771914990928	26.587149945387416	27.028263139365894	23.16686776533741
39	22.62555628104868	26.746323805970373	27.439587652441315	23.188532260539628
40	22.88131768274152	27.104690664107046	27.644497669562284	22.369493983589145
41	22.904486656777227	26.142426002208573	27.343902798631525	23.60918454238267
42	22.60118372394619	27.165772504746634	27.246111674454852	22.986932096852332
43	23.241790811245078	25.89839953541694	27.58762836965647	23.27218128368152
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	8.0
2	13.0
3	51.5
4	90.0
5	90.0
6	138.5
7	187.0
8	192.5
9	198.0
10	253.5
11	309.0
12	309.0
13	531.0
14	753.0
15	1242.0
16	1731.0
17	1954.5
18	2178.0
19	2178.0
20	2026.0
21	1874.0
22	2098.5
23	2323.0
24	3038.5
25	3754.0
26	3754.0
27	4920.5
28	6087.0
29	7579.5
30	9072.0
31	10891.0
32	12710.0
33	12710.0
34	14899.0
35	17088.0
36	18870.0
37	20652.0
38	22261.5
39	23871.0
40	23871.0
41	25064.5
42	26258.0
43	26697.5
44	27137.0
45	26737.0
46	26337.0
47	26337.0
48	25662.0
49	24987.0
50	25126.5
51	25266.0
52	23929.0
53	22592.0
54	22592.0
55	21471.0
56	20350.0
57	17954.0
58	15558.0
59	14087.5
60	12617.0
61	12617.0
62	11173.5
63	9730.0
64	7975.0
65	6220.0
66	5281.5
67	4343.0
68	4343.0
69	3627.5
70	2912.0
71	2407.5
72	1903.0
73	1671.0
74	1439.0
75	1439.0
76	1193.5
77	948.0
78	664.5
79	381.0
80	287.0
81	193.0
82	193.0
83	163.0
84	133.0
85	105.0
86	77.0
87	57.0
88	37.0
89	37.0
90	31.5
91	26.0
92	15.0
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	332341.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.19239855105862
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.75664616805247	63.704266789853456
2	5.165010984700144	7.250890191173366
3	1.3443193544871954	2.830829997302012
4	0.6616206934175493	1.8576297360796954
5	0.40471647511377307	1.4204010060682781
6	0.2597159914304581	1.0938053028342027
7	0.21873173679522423	1.0747313671426943
8	0.15480779507192072	0.8693064360399094
9	0.12978563724360723	0.8198968658045755
>10	0.7947321093507481	10.840977660705587
>50	0.07499949590047916	3.599756657973467
>100	0.034051495265140515	4.005780648138155
>500	4.3103158563469006E-4	0.2722962676226843
>1k	4.3103158563469006E-4	0.35943107326194323
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1188	0.3574641708365805	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	900	0.2708061900277125	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	456	0.137208469614041	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	359	0.10802158024438754	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	341	0.10260545644383329	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	9.02687300092375E-4	0.0	0.0
2	0.0	0.0	0.0012035830667898333	0.0	0.0
3	0.0	0.0	0.0012035830667898333	0.0	0.0
4	0.0	0.0	0.00180537460018475	0.0	0.0
5	0.0	0.0	0.002106270366882208	0.0	0.0
6	0.0	0.0	0.0036107492003695	0.0	0.0
7	0.0	0.0	0.007522394167436459	0.0	0.0
8	3.008957666974583E-4	0.0	0.00902687300092375	0.0	0.0
9	3.008957666974583E-4	0.0	0.009628664534318666	6.017915333949166E-4	0.0
10	3.008957666974583E-4	0.0	0.0108322476011085	6.017915333949166E-4	0.0
11	3.008957666974583E-4	0.0	0.015345684101570375	9.02687300092375E-4	0.0
12	3.008957666974583E-4	0.0	0.016850162935057665	9.02687300092375E-4	0.0
13	3.008957666974583E-4	0.0	0.0180537460018475	9.02687300092375E-4	0.0
14	3.008957666974583E-4	0.0	0.01955822483533479	9.02687300092375E-4	0.0
15	3.008957666974583E-4	0.0	0.02016001636872971	9.02687300092375E-4	0.0
16	3.008957666974583E-4	0.0	0.02136359943551954	9.02687300092375E-4	0.0
17	3.008957666974583E-4	0.0	0.021664495202217	9.02687300092375E-4	0.0
18	3.008957666974583E-4	0.0	0.021965390968914457	0.0012035830667898333	0.0
19	3.008957666974583E-4	0.0	0.02226628673561192	0.0012035830667898333	0.0
20	3.008957666974583E-4	0.0	0.023168974035704292	0.0012035830667898333	0.0
21	3.008957666974583E-4	0.0	0.023770765569099208	0.0012035830667898333	0.0
22	3.008957666974583E-4	0.0	0.024071661335796666	0.0012035830667898333	0.0
23	3.008957666974583E-4	0.0	0.024372557102494124	0.0015044788334872916	0.0
24	3.008957666974583E-4	0.0	0.024673452869191582	0.0024071661335796665	0.0
25	3.008957666974583E-4	0.0	0.024673452869191582	0.0024071661335796665	0.0
26	3.008957666974583E-4	0.0	0.024673452869191582	0.002708061900277125	0.0
27	3.008957666974583E-4	0.0	0.024673452869191582	0.0036107492003695	0.0
28	3.008957666974583E-4	0.0	0.024673452869191582	0.013239413734688166	0.0
29	3.008957666974583E-4	0.0	0.024673452869191582	0.030089576669745835	0.0
30	3.008957666974583E-4	0.0	0.024673452869191582	0.056869299905819626	0.0
31	3.008957666974583E-4	0.0	0.024673452869191582	0.13480130348046132	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTCG	35	5.6152567E-7	37.0	21
AACGGCC	35	2.383239E-5	31.714285	37
GGTATCA	235	0.0	28.340427	1
TTAACGG	40	5.92762E-5	27.750002	35
TCGCCGT	50	9.0657195E-6	25.899998	25
TCCGCCG	50	9.0657195E-6	25.899998	31
CGCCGTC	45	1.3204488E-4	24.666666	26
GAGTTCC	55	1.8973866E-5	23.545454	15
GTATCAA	650	0.0	23.33846	1
CGTGCAA	40	0.0019283143	23.125002	11
CTAACCG	40	0.0019283143	23.125002	6
TAACCGT	40	0.0019283143	23.125002	7
GGGTAAG	50	2.6965048E-4	22.199999	1
TAACGGC	50	2.6965048E-4	22.199999	36
CCGTCCG	50	2.6965048E-4	22.199999	28
TGCCGAG	50	2.6965048E-4	22.199999	11
GAGTCGC	60	3.716034E-5	21.583334	22
TCGAGCT	60	3.716034E-5	21.583334	27
AGTCGCC	60	3.716034E-5	21.583334	23
CACTAAC	80	6.929513E-7	20.8125	32
>>END_MODULE