FastQCFastQC Report
Fri 10 Feb 2017
ERR1632927.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632927.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584473
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24310.4159302482749417No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17380.29736189695674564No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8310.14217936500060738No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC6420.10984254191382666No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC6000.10265658122787537No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGTT250.00549416129.59999825
GGTATCA4250.027.8588241
TATACAG701.9195613E-723.7857135
GTATCAA13800.022.7898561
AGCTTCG909.458381E-820.55555521
GACGATA450.003823592820.55555513
CGATAAC450.003823592820.55555515
GATATAG851.2431665E-619.5882362
GCCGGCA951.6725971E-719.47368415
TGCGGTA701.2178966E-418.536
GCGTTAT609.229411E-418.511
GTCAACC2050.017.14634115
TCGCCGT650.001578678317.07692325
CCGGCAG1201.0385884E-716.95833416
GCCGCTC1107.791459E-716.81818227
CTGGACT1107.791459E-716.8181824
CTACGGT1005.8678525E-616.6527
TGTACTA904.439945E-516.4444455
ATCAACG18950.016.4010563
CCGAGTT803.3790074E-416.187513
Produced by FastQC (version 0.11.5)