##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632927.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	584473
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.726105397511947	33.0	33.0	33.0	27.0	33.0
2	31.259464509053455	33.0	33.0	33.0	27.0	33.0
3	31.669415011471873	33.0	33.0	33.0	27.0	33.0
4	31.80121408516732	33.0	33.0	33.0	27.0	33.0
5	31.905138475173363	33.0	33.0	33.0	33.0	33.0
6	34.785416948259375	37.0	37.0	37.0	27.0	37.0
7	34.91736658494062	37.0	37.0	37.0	27.0	37.0
8	35.12413233802075	37.0	37.0	37.0	33.0	37.0
9	35.17590718476303	37.0	37.0	37.0	33.0	37.0
10	35.219534520841854	37.0	37.0	37.0	33.0	37.0
11	35.267351956377794	37.0	37.0	37.0	33.0	37.0
12	35.17651114764925	37.0	37.0	37.0	33.0	37.0
13	35.22409760587743	37.0	37.0	37.0	33.0	37.0
14	35.19096861617218	37.0	37.0	37.0	33.0	37.0
15	35.23314507256965	37.0	37.0	37.0	33.0	37.0
16	35.2142631053958	37.0	37.0	37.0	33.0	37.0
17	35.208502360245895	37.0	37.0	37.0	33.0	37.0
18	35.183902421497656	37.0	37.0	37.0	33.0	37.0
19	35.221122275964845	37.0	37.0	37.0	33.0	37.0
20	35.153447635733386	37.0	37.0	37.0	33.0	37.0
21	35.222349022110514	37.0	37.0	37.0	33.0	37.0
22	35.23395948144739	37.0	37.0	37.0	33.0	37.0
23	35.20553900693445	37.0	37.0	37.0	33.0	37.0
24	35.19906822043105	37.0	37.0	37.0	33.0	37.0
25	35.21709642703769	37.0	37.0	37.0	33.0	37.0
26	35.16825413663249	37.0	37.0	37.0	33.0	37.0
27	35.099741134321	37.0	37.0	37.0	33.0	37.0
28	35.07987879679643	37.0	37.0	37.0	33.0	37.0
29	35.1252153649527	37.0	37.0	37.0	33.0	37.0
30	35.09190330434426	37.0	37.0	37.0	33.0	37.0
31	35.07747834373872	37.0	37.0	37.0	27.0	37.0
32	35.111868298450055	37.0	37.0	37.0	33.0	37.0
33	35.08379685631329	37.0	37.0	37.0	33.0	37.0
34	35.01694175778864	37.0	37.0	37.0	27.0	37.0
35	35.006532380452136	37.0	37.0	37.0	27.0	37.0
36	34.99653362944054	37.0	37.0	37.0	27.0	37.0
37	35.00263998508058	37.0	37.0	37.0	27.0	37.0
38	34.87084604421419	37.0	37.0	37.0	27.0	37.0
39	34.92160287985929	37.0	37.0	37.0	27.0	37.0
40	34.95869441359994	37.0	37.0	37.0	27.0	37.0
41	34.97541032690988	37.0	37.0	37.0	27.0	37.0
42	34.833242938510416	37.0	37.0	37.0	27.0	37.0
43	34.66542509234815	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	10.0
16	34.0
17	26.0
18	24.0
19	15.0
20	50.0
21	111.0
22	298.0
23	766.0
24	1604.0
25	3011.0
26	4872.0
27	7335.0
28	10219.0
29	13832.0
30	18377.0
31	23823.0
32	29983.0
33	39189.0
34	55355.0
35	100727.0
36	274812.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.97691424582487	20.328227309045925	13.561105474504382	25.13375297062482
2	16.219226551098167	22.875992560819746	37.63869331859641	23.266087569485673
3	17.581992666898213	27.22332768151822	30.007031975814108	25.187647675769455
4	12.619402436040673	18.10690998557675	39.21139214300746	30.062295435375113
5	13.931524638434967	36.89699951922501	35.369982873460366	13.801492968879659
6	29.263285044818154	40.054202674888316	17.427836700754355	13.254675579539175
7	26.722876848032328	31.8678536048714	23.881171585342692	17.52809796175358
8	24.510114239665477	35.498132505693164	20.697448812862184	19.294304441779175
9	26.155357048144225	15.114299548482139	20.233783254316283	38.49656014905736
10	15.21131001774248	27.91643069910843	33.938265753935596	22.9339935292135
11	33.99027842175772	23.74652036963213	24.109582478574715	18.153618730035433
12	23.01064377653031	25.809062180802194	29.883501889736564	21.296792152930934
13	28.31182963113779	22.069625115274786	25.792123844899596	23.826421408687825
14	22.309841515348015	20.75767400718254	27.526677879046595	29.405806598422853
15	24.14037945294308	28.516800604989452	24.301037002564705	23.041782939502767
16	23.116208960892976	27.96690351821213	25.271654978074267	23.645232542820626
17	22.5038624538687	27.167550939051075	26.3452717234158	23.98331488366443
18	23.24247655580326	26.85564602642038	27.544984969365565	22.35689244841079
19	24.849394240623603	25.899399972282723	27.344804635971208	21.906401151122466
20	24.23550788488091	26.203605641321325	27.17182829660224	22.389058177195526
21	23.159838007914825	26.83117954122774	27.210495608864736	22.798486841992702
22	22.959486580218417	26.540490322050804	27.266614539935972	23.2334085577948
23	23.016118794195798	26.657518824650584	27.751837980539733	22.574524400613885
24	23.107483151488605	27.041625532744884	26.244839368114526	23.606051947651988
25	23.524611059877873	26.59421393289339	27.630018837482655	22.25115616974608
26	23.287816545845573	26.92819001048808	27.296727137096156	22.48726630657019
27	23.087122929545078	26.81543886543946	26.903723525295437	23.19371467972002
28	22.89139104800393	26.58309280326037	27.589640582199692	22.93587556653601
29	23.93540847909142	26.52474964626253	26.94033770593338	22.59950416871267
30	23.60690741916222	26.49669018072691	27.04521851308786	22.851183887023012
31	23.335038573210394	26.55109816877769	27.280815367005832	22.833047891006085
32	22.443295070944252	26.475303392971107	27.43103616420262	23.65036537188202
33	22.56323217667882	26.542714547977408	27.853810184559425	23.040243090784347
34	23.29534469513562	25.98426274609777	27.468505816350795	23.251886742415817
35	22.983268688202877	26.952143212774587	27.29193649663885	22.772651602383686
36	23.642323939685834	26.624155435751522	26.707820549452244	23.025700075110397
37	23.188068567752488	26.038157451242398	27.22452534163255	23.549248639372564
38	23.223998371182244	26.627748416094498	26.92545250165534	23.22280071106792
39	22.62602378552987	26.362552247922487	27.721725383379557	23.289698583168082
40	22.85323701864757	26.786010645487472	27.759195035527735	22.601557300337227
41	23.038018864857744	25.96236267543582	27.36294063198813	23.6366778277183
42	22.568707194344306	27.006893389429454	27.322220188101078	23.102179228125166
43	23.24641172475033	25.815905952884048	27.58467884744034	23.353003474925274
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	15.0
2	25.0
3	133.5
4	242.0
5	242.0
6	311.5
7	381.0
8	383.5
9	386.0
10	514.0
11	642.0
12	642.0
13	997.0
14	1352.0
15	2238.5
16	3125.0
17	3561.0
18	3997.0
19	3997.0
20	3483.5
21	2970.0
22	3166.0
23	3362.0
24	4537.5
25	5713.0
26	5713.0
27	7529.0
28	9345.0
29	12089.5
30	14834.0
31	17823.5
32	20813.0
33	20813.0
34	24743.5
35	28674.0
36	31917.0
37	35160.0
38	38611.0
39	42062.0
40	42062.0
41	45008.0
42	47954.0
43	49162.0
44	50370.0
45	49896.5
46	49423.0
47	49423.0
48	48006.0
49	46589.0
50	46228.5
51	45868.0
52	42890.0
53	39912.0
54	39912.0
55	37718.0
56	35524.0
57	30979.5
58	26435.0
59	24557.5
60	22680.0
61	22680.0
62	19633.5
63	16587.0
64	13490.0
65	10393.0
66	8965.0
67	7537.0
68	7537.0
69	6232.5
70	4928.0
71	3948.0
72	2968.0
73	2427.0
74	1886.0
75	1886.0
76	1428.5
77	971.0
78	778.5
79	586.0
80	461.5
81	337.0
82	337.0
83	277.0
84	217.0
85	168.5
86	120.0
87	93.5
88	67.0
89	67.0
90	49.0
91	31.0
92	16.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	584473.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.839140483028736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.82085346830611	48.48001833673198
2	6.754171241371459	7.542942335867471
3	1.9972222140011975	3.3456951535031565
4	0.9888343244496908	2.208626350295484
5	0.6242055053804177	1.7427549452608548
6	0.4192478715104803	1.4046264476690695
7	0.31539429780469075	1.232794255186363
8	0.23594371749229376	1.0539915509712188
9	0.19681240346689416	0.9890851901391393
>10	1.3869187007982873	15.19861857967133
>50	0.16671523734778892	6.499945688023032
>100	0.09090756687780212	8.858571955329706
>500	0.002157128705574959	0.724950246650298
>1k	6.163224873071312E-4	0.7173789647009475
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2431	0.4159302482749417	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1738	0.29736189695674564	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	831	0.14217936500060738	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	642	0.10984254191382666	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	600	0.10265658122787537	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	8.554715102322947E-4	0.0	0.0
2	1.7109430204645894E-4	0.0	8.554715102322947E-4	0.0	0.0
3	1.7109430204645894E-4	0.0	8.554715102322947E-4	0.0	0.0
4	1.7109430204645894E-4	0.0	0.0010265658122787537	0.0	0.0
5	1.7109430204645894E-4	0.0	0.0018820373225110485	0.0	0.0
6	1.7109430204645894E-4	0.0	0.003592980342975638	0.0	0.0
7	1.7109430204645894E-4	0.0	0.009410186612555242	0.0	0.0
8	1.7109430204645894E-4	0.0	0.011976601143252127	0.0	0.0
9	1.7109430204645894E-4	0.0	0.01454301567394901	3.421886040929179E-4	0.0
10	0.0011976601143252126	0.0	0.017280524506692353	3.421886040929179E-4	0.0
11	0.0011976601143252126	0.0	0.023953202286504254	3.421886040929179E-4	0.0
12	0.0011976601143252126	0.0	0.024979768098783005	3.421886040929179E-4	0.0
13	0.0011976601143252126	0.0	0.02600633391106176	3.421886040929179E-4	0.0
14	0.0011976601143252126	0.0	0.02788837123357281	3.421886040929179E-4	0.0
15	0.0011976601143252126	0.0	0.031310257274501985	3.421886040929179E-4	0.0
16	0.0011976601143252126	0.0	0.03421886040929179	5.132829061393769E-4	0.0
17	0.0011976601143252126	0.0	0.03473214331543117	5.132829061393769E-4	0.0
18	0.0011976601143252126	0.0	0.03575870912770992	6.843772081858358E-4	0.0
19	0.0011976601143252126	0.0	0.03695636924203513	6.843772081858358E-4	0.0
20	0.0011976601143252126	0.0	0.03729855784612805	6.843772081858358E-4	0.0
21	0.0011976601143252126	0.0	0.039522783772732016	6.843772081858358E-4	0.0
22	0.0011976601143252126	0.0	0.03986497237682494	0.0013687544163716715	0.0
23	0.0011976601143252126	0.0	0.040378255282964313	0.0015398487184181305	0.0
24	0.0011976601143252126	0.0	0.04072044388705723	0.0018820373225110485	0.0
25	0.0011976601143252126	0.0	0.04072044388705723	0.002224225926603966	0.0
26	0.0011976601143252126	0.0	0.04072044388705723	0.002908603134789802	0.0
27	0.0011976601143252126	0.0	0.04089153818910369	0.0047906404573008505	0.0
28	0.0011976601143252126	0.0	0.04106263249115015	0.01471410997599547	0.0
29	0.0011976601143252126	0.0	0.04106263249115015	0.03592980342975638	0.0
30	0.0011976601143252126	0.0	0.04106263249115015	0.06912209802676941	0.0
31	0.0011976601143252126	0.0	0.04106263249115015	0.1541559661438595	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGGTT	25	0.005494161	29.599998	25
GGTATCA	425	0.0	27.858824	1
TATACAG	70	1.9195613E-7	23.785713	5
GTATCAA	1380	0.0	22.789856	1
AGCTTCG	90	9.458381E-8	20.555555	21
GACGATA	45	0.0038235928	20.555555	13
CGATAAC	45	0.0038235928	20.555555	15
GATATAG	85	1.2431665E-6	19.588236	2
GCCGGCA	95	1.6725971E-7	19.473684	15
TGCGGTA	70	1.2178966E-4	18.5	36
GCGTTAT	60	9.229411E-4	18.5	11
GTCAACC	205	0.0	17.146341	15
TCGCCGT	65	0.0015786783	17.076923	25
CCGGCAG	120	1.0385884E-7	16.958334	16
GCCGCTC	110	7.791459E-7	16.818182	27
CTGGACT	110	7.791459E-7	16.818182	4
CTACGGT	100	5.8678525E-6	16.65	27
TGTACTA	90	4.439945E-5	16.444445	5
ATCAACG	1895	0.0	16.401056	3
CCGAGTT	80	3.3790074E-4	16.1875	13
>>END_MODULE