FastQCFastQC Report
Fri 10 Feb 2017
ERR1632926.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632926.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490828
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12170.2479483648039639No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8830.17990008719958928No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC6710.1367077672830401No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC6150.12529847522961202No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG5900.12020504127718876No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG5570.11348170845999006No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG5380.10961069865614839No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG5370.10940696129805144No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA5210.10614716356850057No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC5040.10268362848085276No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT358.8621234E-426.42857225
TATAGTG901.4188117E-1024.6666665
GGTATCA3150.024.0793651
GTATCAA8700.023.8160931
CTACGCA400.001929619723.1259
CCGCCGT502.6990465E-422.224
AACGATT705.092097E-621.14285922
GTCTTAT450.00382273220.5555551
TACCGGG450.00382273220.55555532
GACCGTT555.1373243E-420.1818187
TAATACT2400.020.0416684
TTAGCCG656.891834E-519.92307729
ACGGGTA656.891834E-519.92307716
GGACCGT656.891834E-519.9230776
TAGGACC1303.274181E-1119.9230774
TCTAATA1650.019.0606062
AGCCGGT701.2173915E-418.531
TTTCGCT500.0070292818.518
TAGCCGG701.2173915E-418.530
CGCCTAT500.0070292818.536
Produced by FastQC (version 0.11.5)