##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632926.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	490828
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.690824484340748	33.0	33.0	33.0	27.0	33.0
2	31.244672268085765	33.0	33.0	33.0	27.0	33.0
3	31.671897283773543	33.0	33.0	33.0	27.0	33.0
4	31.80206508186167	33.0	33.0	33.0	27.0	33.0
5	31.90949171603902	33.0	33.0	33.0	33.0	33.0
6	34.818298874554834	37.0	37.0	37.0	27.0	37.0
7	34.94369718108992	37.0	37.0	37.0	27.0	37.0
8	35.161938601709764	37.0	37.0	37.0	33.0	37.0
9	35.1936340225089	37.0	37.0	37.0	33.0	37.0
10	35.25521160162012	37.0	37.0	37.0	33.0	37.0
11	35.308953441938925	37.0	37.0	37.0	33.0	37.0
12	35.2094358920029	37.0	37.0	37.0	33.0	37.0
13	35.26266227680573	37.0	37.0	37.0	33.0	37.0
14	35.21147122821029	37.0	37.0	37.0	33.0	37.0
15	35.267609019860316	37.0	37.0	37.0	33.0	37.0
16	35.23304905180633	37.0	37.0	37.0	33.0	37.0
17	35.247760926434516	37.0	37.0	37.0	33.0	37.0
18	35.222134841533084	37.0	37.0	37.0	33.0	37.0
19	35.245391460959844	37.0	37.0	37.0	33.0	37.0
20	35.160911765424956	37.0	37.0	37.0	33.0	37.0
21	35.21766280652285	37.0	37.0	37.0	33.0	37.0
22	35.22889484707474	37.0	37.0	37.0	33.0	37.0
23	35.2286849975959	37.0	37.0	37.0	33.0	37.0
24	35.22837327943801	37.0	37.0	37.0	33.0	37.0
25	35.20848851328775	37.0	37.0	37.0	33.0	37.0
26	35.194051684093004	37.0	37.0	37.0	33.0	37.0
27	35.107844295761446	37.0	37.0	37.0	33.0	37.0
28	35.131335213150024	37.0	37.0	37.0	33.0	37.0
29	35.15450830025997	37.0	37.0	37.0	33.0	37.0
30	35.12489915000774	37.0	37.0	37.0	33.0	37.0
31	35.10165475482246	37.0	37.0	37.0	33.0	37.0
32	35.147750332091896	37.0	37.0	37.0	33.0	37.0
33	35.11629328400173	37.0	37.0	37.0	33.0	37.0
34	35.082827385560726	37.0	37.0	37.0	33.0	37.0
35	35.06263497599974	37.0	37.0	37.0	27.0	37.0
36	35.05095063851288	37.0	37.0	37.0	27.0	37.0
37	35.07851019094265	37.0	37.0	37.0	33.0	37.0
38	34.929726910445204	37.0	37.0	37.0	27.0	37.0
39	34.985997131378	37.0	37.0	37.0	27.0	37.0
40	35.036523996186034	37.0	37.0	37.0	27.0	37.0
41	35.06435859404924	37.0	37.0	37.0	33.0	37.0
42	34.94494201634789	37.0	37.0	37.0	27.0	37.0
43	34.76827116627413	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	6.0
16	26.0
17	17.0
18	25.0
19	27.0
20	25.0
21	73.0
22	286.0
23	684.0
24	1334.0
25	2433.0
26	3966.0
27	6017.0
28	8411.0
29	11599.0
30	14982.0
31	19056.0
32	24534.0
33	32305.0
34	46189.0
35	84750.0
36	234083.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.94020716014571	20.380051667794014	13.231315246888931	25.448425925171342
2	16.243979561068237	22.68045017806645	37.57303984287775	23.502530417987565
3	17.692755914495507	26.42921756704996	30.323860904430884	25.55416561402365
4	12.733381143700035	17.987971346377957	38.75247540890088	30.52617210102113
5	14.06093376905963	36.876461815544346	35.36085960866128	13.701744806734743
6	30.08691435696415	39.67031220712755	17.22395625351447	13.018817182393832
7	26.877236017505112	31.947647648463413	23.49947435761611	17.675641976415363
8	24.472523979887047	36.09431409781023	20.509221152827468	18.92394076947525
9	26.044357697604863	15.21367974117206	20.15838542218455	38.583577139038525
10	14.972047234469102	27.87677149632865	34.009469712404346	23.1417115567979
11	34.43242846781357	23.088943581050795	24.166102993309266	18.31252495782637
12	23.41777567701924	25.49385935602696	30.078764862640273	21.009600104313527
13	27.771846756908737	21.98224225186827	25.896444375626494	24.3494666155965
14	21.932122861776428	20.737610731254126	27.21197649685837	30.11828991011108
15	24.1504152167358	28.558272959162885	24.08603421157717	23.205277612524142
16	23.355024570725387	28.109847033991542	25.031579290505025	23.50354910477805
17	22.64316624153471	27.087085496344955	26.32449656498814	23.945251697132193
18	23.108706104786197	27.17428508561044	27.52797313926671	22.189035670336654
19	24.93093303560514	26.174342132070706	26.715672292534247	22.179052539789907
20	24.281622075350224	26.36361413774275	26.852176322459194	22.50258746444783
21	23.3407629556586	26.914112479320657	27.018833481382483	22.726291083638262
22	23.02822984833791	26.955471163014334	26.763346834328928	23.252952154318823
23	23.14639751603413	26.79451865011776	27.326884366825038	22.73219946702307
24	23.660019395796493	27.164301955063692	25.708191056744926	23.467487592394892
25	23.549593747707956	26.774144914308067	27.05530246848183	22.620958869502147
26	23.55448344430228	27.00966530026812	26.810206426691224	22.625644828738377
27	23.29777437310015	26.94711793133236	26.348945047959777	23.40616264760772
28	22.95977409601734	26.646197853423192	27.108274181587035	23.28575386897243
29	24.35415257483273	26.70833774764276	26.262152933410484	22.675356744114026
30	23.83258493810459	26.314513434441395	26.94813661812285	22.90476500933117
31	23.414312141931592	26.609728866323845	26.840767030405765	23.1351919613388
32	22.633794323062254	26.43410726364429	27.130685290977695	23.801413122315758
33	22.640313918521358	26.519473216686904	27.701149893649095	23.13906297114264
34	23.372953458237916	26.181269202246	27.34094224453373	23.104835094982356
35	23.311221038734548	26.94243197209613	26.8051129927388	22.941233996430523
36	24.15652733747871	26.75132633020121	26.34262918985877	22.749517142461308
37	23.09994539838803	26.413733527834598	26.78596168107769	23.70035939269968
38	23.44833628073378	26.544532911732826	26.505211601620122	23.501919205913275
39	23.054919442248607	26.462223019061664	27.01435125950435	23.468506279185377
40	23.050233483012377	27.110107817809904	27.15778235960459	22.68187633957313
41	23.121541558346305	26.030299819896175	27.128647917396727	23.719510704360793
42	22.821640167227624	26.86175197828975	27.30100972234673	23.0155981321359
43	23.540833041309785	25.65766419193689	27.134963775497727	23.666538991255592
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	12.0
2	20.0
3	76.0
4	132.0
5	132.0
6	164.0
7	196.0
8	184.5
9	173.0
10	233.5
11	294.0
12	294.0
13	469.5
14	645.0
15	1156.5
16	1668.0
17	1893.5
18	2119.0
19	2119.0
20	2004.5
21	1890.0
22	2286.5
23	2683.0
24	3732.0
25	4781.0
26	4781.0
27	6338.5
28	7896.0
29	9984.5
30	12073.0
31	15369.5
32	18666.0
33	18666.0
34	21734.5
35	24803.0
36	27808.5
37	30814.0
38	33663.0
39	36512.0
40	36512.0
41	38519.0
42	40526.0
43	41506.5
44	42487.0
45	41459.0
46	40431.0
47	40431.0
48	39089.5
49	37748.0
50	37609.5
51	37471.0
52	35712.5
53	33954.0
54	33954.0
55	32096.0
56	30238.0
57	26249.0
58	22260.0
59	20670.5
60	19081.0
61	19081.0
62	16560.0
63	14039.0
64	11844.0
65	9649.0
66	8131.5
67	6614.0
68	6614.0
69	5547.5
70	4481.0
71	3573.5
72	2666.0
73	2163.5
74	1661.0
75	1661.0
76	1291.5
77	922.0
78	709.5
79	497.0
80	416.5
81	336.0
82	336.0
83	263.0
84	190.0
85	157.0
86	124.0
87	87.5
88	51.0
89	51.0
90	39.5
91	28.0
92	15.0
93	2.0
94	2.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	490828.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.09753239864111
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.17242029929714	55.63462146501502
2	6.56672670271377	8.286885017550077
3	1.875506936645976	3.5501957909668675
4	0.8927117175756535	2.2531162608950535
5	0.4821281244296331	1.521054747574742
6	0.34974775640587796	1.324093223470315
7	0.25026548968671264	1.1053794390638392
8	0.17183608607057738	0.8673946406475149
9	0.14304878062353915	0.8123422562981878
>10	0.9222259837688374	11.249172966186487
>50	0.10617219896615472	4.716895024378818
>100	0.06396306759322745	7.319955639849969
>500	0.0029221706007058227	1.109568422765951
>1k	3.246856223006469E-4	0.2493251053371792
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1217	0.2479483648039639	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	883	0.17990008719958928	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	671	0.1367077672830401	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	615	0.12529847522961202	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	590	0.12020504127718876	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	557	0.11348170845999006	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	538	0.10961069865614839	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	537	0.10940696129805144	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	521	0.10614716356850057	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	504	0.10268362848085276	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	6.112120742907903E-4	0.0	0.0
2	0.0	0.0	6.112120742907903E-4	2.037373580969301E-4	0.0
3	0.0	0.0	8.149494323877204E-4	2.037373580969301E-4	0.0
4	0.0	0.0	8.149494323877204E-4	2.037373580969301E-4	0.0
5	0.0	0.0	0.0010186867904846504	6.112120742907903E-4	0.0
6	0.0	0.0	0.0016298988647754408	6.112120742907903E-4	0.0
7	0.0	0.0	0.0032597977295508816	8.149494323877204E-4	0.0
8	0.0	0.0	0.0036672724457447415	8.149494323877204E-4	2.037373580969301E-4
9	0.0	0.0	0.005093433952423252	0.0010186867904846504	2.037373580969301E-4
10	6.112120742907903E-4	0.0	0.005704646026714042	0.0012224241485815806	2.037373580969301E-4
11	0.0010186867904846504	0.0	0.011001817337234225	0.0012224241485815806	2.037373580969301E-4
12	0.0010186867904846504	0.0	0.012020504127718875	0.0012224241485815806	2.037373580969301E-4
13	0.0010186867904846504	0.0	0.012835453560106595	0.0012224241485815806	2.037373580969301E-4
14	0.0010186867904846504	0.0	0.013854140350591246	0.0012224241485815806	2.037373580969301E-4
15	0.0010186867904846504	0.0	0.014872827141075896	0.0012224241485815806	2.037373580969301E-4
16	0.0010186867904846504	0.0	0.016095251289657476	0.0012224241485815806	2.037373580969301E-4
17	0.0010186867904846504	0.0	0.01670646336394827	0.0016298988647754408	2.037373580969301E-4
18	0.0010186867904846504	0.0	0.01670646336394827	0.0018336362228723708	2.037373580969301E-4
19	0.0010186867904846504	0.0	0.016910200722045198	0.0018336362228723708	2.037373580969301E-4
20	0.0010186867904846504	0.0	0.017317675438239057	0.0022411109390662307	2.037373580969301E-4
21	0.0010186867904846504	0.0	0.018743836944917568	0.002444848297163161	2.037373580969301E-4
22	0.0010186867904846504	0.0	0.018743836944917568	0.002852323013357021	2.037373580969301E-4
23	0.0010186867904846504	0.0	0.018743836944917568	0.002852323013357021	2.037373580969301E-4
24	0.0010186867904846504	0.0	0.018743836944917568	0.0032597977295508816	2.037373580969301E-4
25	0.0010186867904846504	0.0	0.018947574303014497	0.0034635350876478115	2.037373580969301E-4
26	0.0010186867904846504	0.0	0.018947574303014497	0.0036672724457447415	2.037373580969301E-4
27	0.0010186867904846504	0.0	0.018947574303014497	0.0044822218781324615	4.074747161938602E-4
28	0.0010186867904846504	0.0	0.018947574303014497	0.010390605262943435	4.074747161938602E-4
29	0.0010186867904846504	0.0	0.019151311661111427	0.02526343240401933	4.074747161938602E-4
30	0.0010186867904846504	0.0	0.019151311661111427	0.05134181424042638	4.074747161938602E-4
31	0.0010186867904846504	0.0	0.019151311661111427	0.12387231372293349	6.112120742907903E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCGTT	35	8.8621234E-4	26.428572	25
TATAGTG	90	1.4188117E-10	24.666666	5
GGTATCA	315	0.0	24.079365	1
GTATCAA	870	0.0	23.816093	1
CTACGCA	40	0.0019296197	23.125	9
CCGCCGT	50	2.6990465E-4	22.2	24
AACGATT	70	5.092097E-6	21.142859	22
GTCTTAT	45	0.003822732	20.555555	1
TACCGGG	45	0.003822732	20.555555	32
GACCGTT	55	5.1373243E-4	20.181818	7
TAATACT	240	0.0	20.041668	4
TTAGCCG	65	6.891834E-5	19.923077	29
ACGGGTA	65	6.891834E-5	19.923077	16
GGACCGT	65	6.891834E-5	19.923077	6
TAGGACC	130	3.274181E-11	19.923077	4
TCTAATA	165	0.0	19.060606	2
AGCCGGT	70	1.2173915E-4	18.5	31
TTTCGCT	50	0.00702928	18.5	18
TAGCCGG	70	1.2173915E-4	18.5	30
CGCCTAT	50	0.00702928	18.5	36
>>END_MODULE