FastQCFastQC Report
Fri 10 Feb 2017
ERR1632925.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632925.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301421
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6120.20303827536900218No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC5540.18379608587324706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4650.1542692778538987No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4640.15393751596604086No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG4570.15161518275103594No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG4330.1436528974424476No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA4310.14298937366673192No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC4060.13469532647028573No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT3970.13170946947956513No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC3910.12971889815241805No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG3830.12706480304955528No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA3780.12540599361026603No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG3660.12142485095597189No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT3630.12042956529239833No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC3540.11744370830167772No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG3420.11346256564738355No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG3410.11313080375952571No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA3350.11114023243237864No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC3260.10815437544165801No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG3130.103841470899506No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCTC250.005489991529.627
TTCGCCG250.005489991529.624
GGTATCA1700.026.1176471
GTATCAA4500.024.6666661
CCAGGAC1550.022.6774183
TTAGGAC1107.2759576E-1121.8636363
ATTATAC806.925111E-720.81253
TAGGACC806.925111E-720.81254
TTTAGGA909.420182E-820.5555552
ACCTAAG450.003819360920.5555551
AGGACAG1900.020.4473695
GCATCAG1403.6379788E-1219.82142815
TCCAGGA1500.019.7333352
ATCAAGC1501.2732926E-1118.50000230
TCGGGGG609.2152803E-418.533
TTATACC500.0070231418.54
CACCTAG500.0070231418.527
CGGGGGT500.0070231418.534
CGCTCTC500.0070231418.529
GAGACCG500.0070231418.521
Produced by FastQC (version 0.11.5)