##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632925.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	301421
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.013592284545535	33.0	33.0	33.0	27.0	33.0
2	30.712319314181826	33.0	33.0	33.0	27.0	33.0
3	31.168651155692537	33.0	33.0	33.0	27.0	33.0
4	31.331436761207748	33.0	33.0	33.0	27.0	33.0
5	31.453634617362425	33.0	33.0	33.0	27.0	33.0
6	34.09651948603448	37.0	33.0	37.0	27.0	37.0
7	34.29779942339784	37.0	33.0	37.0	27.0	37.0
8	34.44986248469748	37.0	37.0	37.0	27.0	37.0
9	34.52802558547679	37.0	37.0	37.0	27.0	37.0
10	34.533592549955046	37.0	37.0	37.0	27.0	37.0
11	34.615862862906035	37.0	37.0	37.0	27.0	37.0
12	34.57015270999698	37.0	37.0	37.0	27.0	37.0
13	34.589995388509756	37.0	37.0	37.0	27.0	37.0
14	34.58358574883635	37.0	37.0	37.0	27.0	37.0
15	34.61228646975493	37.0	37.0	37.0	27.0	37.0
16	34.61261159640503	37.0	37.0	37.0	27.0	37.0
17	34.56758487298496	37.0	37.0	37.0	27.0	37.0
18	34.574857093566806	37.0	37.0	37.0	27.0	37.0
19	34.578078501497906	37.0	37.0	37.0	27.0	37.0
20	34.56857684102965	37.0	37.0	37.0	27.0	37.0
21	34.570189203804645	37.0	37.0	37.0	27.0	37.0
22	34.57467130690961	37.0	37.0	37.0	27.0	37.0
23	34.554002541296065	37.0	37.0	37.0	27.0	37.0
24	34.543366255171335	37.0	37.0	37.0	27.0	37.0
25	34.51972490304259	37.0	37.0	37.0	27.0	37.0
26	34.47757123757137	37.0	37.0	37.0	27.0	37.0
27	34.40353193705813	37.0	37.0	37.0	27.0	37.0
28	34.36563477660813	37.0	37.0	37.0	27.0	37.0
29	34.426396966369296	37.0	37.0	37.0	27.0	37.0
30	34.359517087396036	37.0	37.0	37.0	27.0	37.0
31	34.36361434671108	37.0	37.0	37.0	27.0	37.0
32	34.37813224692374	37.0	37.0	37.0	27.0	37.0
33	34.37170270153705	37.0	37.0	37.0	27.0	37.0
34	34.28177532421431	37.0	37.0	37.0	27.0	37.0
35	34.287557933919665	37.0	37.0	37.0	27.0	37.0
36	34.2510807143497	37.0	37.0	37.0	27.0	37.0
37	34.27763493585384	37.0	37.0	37.0	27.0	37.0
38	34.141974182289886	37.0	37.0	37.0	27.0	37.0
39	34.22133162586548	37.0	37.0	37.0	27.0	37.0
40	34.25971315867176	37.0	37.0	37.0	27.0	37.0
41	34.287282571552744	37.0	37.0	37.0	27.0	37.0
42	34.191021859790794	37.0	37.0	37.0	27.0	37.0
43	33.91855577414978	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	10.0
16	17.0
17	20.0
18	19.0
19	30.0
20	76.0
21	170.0
22	446.0
23	943.0
24	1727.0
25	2965.0
26	4318.0
27	5960.0
28	8003.0
29	10178.0
30	12603.0
31	15567.0
32	19299.0
33	24170.0
34	33111.0
35	55855.0
36	105933.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.096078242723635	21.09043497301117	13.142415425600737	25.67107135866446
2	16.559894632424417	23.095935585111853	37.03889244611357	23.305277336350155
3	17.757555047591243	27.361729939188045	29.79420810096178	25.086506912258933
4	13.130471997637855	18.542835436150764	38.75509669200222	29.571595874209162
5	14.223295656241602	36.99476811502848	35.64681956466205	13.135116664067864
6	29.765344816718148	40.04631395954495	17.159056601895685	13.029284621841212
7	26.93143477063642	31.997770560113597	23.68713526927454	17.38365939997545
8	23.9581847316544	36.49579823569028	20.46937671894128	19.07664031371404
9	25.82899001728479	15.271663221872398	20.158847591906337	38.74049916893647
10	15.311142886527481	27.978807050603642	33.94952574638131	22.760524316487572
11	34.01985926660717	23.816522405539096	23.82846583350198	18.335152494351753
12	23.32120190696733	25.794155019059723	29.602781491667802	21.281861582305147
13	27.241300373895648	22.049890352696064	26.130561573347578	24.57824770006071
14	21.9858603083395	20.671087946758853	27.356421748982317	29.98662999591933
15	23.78301445486545	28.66422711091795	24.503269513404838	23.049488920811754
16	23.30461381257444	28.216348562309857	24.75010035797108	23.728937267144627
17	22.337196147580958	27.713065778429506	25.942120821044316	24.007617252945217
18	22.552177850912845	27.77543701334678	27.34845946367373	22.323925672066643
19	24.77266016634541	26.18563404673198	26.653418308611542	22.388287478311067
20	23.72031145806032	26.59535997823642	27.35542646331875	22.32890210038451
21	23.197122960908498	27.274144800793575	27.00641295729229	22.522319281005636
22	22.961572020529424	26.98219433947867	26.80967815779259	23.246555482199316
23	22.893229071630707	27.00973057617087	27.464576124423978	22.63246422777444
24	23.263807100367924	27.773114680131776	25.310114424675124	23.652963794825176
25	23.363999190500994	26.988497815347966	26.882334011233453	22.76516898291758
26	23.2372661493393	27.57472107119278	26.853802488877683	22.334210290590235
27	23.28902100384512	27.03593976531164	26.128239240132572	23.546799990710667
28	22.575401183062894	26.783468968651817	27.86833034194698	22.772799506338313
29	24.43525832639398	26.77749725467038	26.37175246582023	22.41549195311541
30	23.55841165678569	26.384359417558827	27.12120257049111	22.936026355164373
31	23.21337929341353	26.80038882493257	26.89892210562635	23.08730977602755
32	22.355111289525283	26.616260977171464	27.107932094976796	23.92069563832646
33	22.542556756164966	26.844181394129805	27.37632746225379	23.236934387451438
34	23.459214852316197	26.5585344086842	27.37765450980522	22.60459622919438
35	23.278736385321526	27.255897896961397	26.656072403714404	22.809293314002673
36	23.980412778140874	27.08537228660246	25.944774916147185	22.989440019109487
37	22.83185312237701	26.463982270644713	26.748965732314606	23.955198874663676
38	23.424048092203265	26.706831972556657	26.5797671695071	23.28935276573298
39	22.544215565604254	27.13049190335113	27.21077828021273	23.114514250831895
40	22.798345171703367	27.40253665139456	27.16997156800621	22.629146608895866
41	22.732324556019652	26.2562993288457	27.328553750402264	23.682822364732385
42	22.443691713583327	27.38163565245952	27.005417671628717	23.169254962328438
43	23.645665033292303	25.539361889184892	27.240968612007787	23.57400446551501
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	5.5
2	7.0
3	30.5
4	54.0
5	54.0
6	78.5
7	103.0
8	97.0
9	91.0
10	144.0
11	197.0
12	197.0
13	276.0
14	355.0
15	626.5
16	898.0
17	1021.0
18	1144.0
19	1144.0
20	1133.5
21	1123.0
22	1278.0
23	1433.0
24	2114.0
25	2795.0
26	2795.0
27	3769.5
28	4744.0
29	6049.0
30	7354.0
31	9439.0
32	11524.0
33	11524.0
34	13854.5
35	16185.0
36	18300.0
37	20415.0
38	22363.0
39	24311.0
40	24311.0
41	25452.5
42	26594.0
43	26933.5
44	27273.0
45	26180.5
46	25088.0
47	25088.0
48	23800.5
49	22513.0
50	22308.0
51	22103.0
52	20585.0
53	19067.0
54	19067.0
55	18585.0
56	18103.0
57	15526.0
58	12949.0
59	11687.0
60	10425.0
61	10425.0
62	9405.5
63	8386.0
64	6767.5
65	5149.0
66	4263.5
67	3378.0
68	3378.0
69	2824.0
70	2270.0
71	1952.0
72	1634.0
73	1561.0
74	1488.0
75	1488.0
76	1368.0
77	1248.0
78	901.0
79	554.0
80	373.0
81	192.0
82	192.0
83	158.0
84	124.0
85	105.0
86	86.0
87	63.0
88	40.0
89	40.0
90	29.5
91	19.0
92	10.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	301421.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.82522900892493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.73293741550526	54.58026467941636
2	5.2241428046726694	6.35519364939085
3	1.450095513994973	2.646071751706768
4	0.7731937844366285	1.8811875602654107
5	0.4830771338711134	1.469163864834278
6	0.3319806028306824	1.2115677716218327
7	0.24033910505414569	1.0233076773302934
8	0.19966278101656193	0.9715627503912968
9	0.16664035637836114	0.9122344054958412
>10	1.1728553562872808	14.065766165387394
>50	0.15276839616571045	6.466161730458758
>100	0.071219430240954	8.031942429738814
>500	0.0010873195456634196	0.38557556396210046
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	612	0.20303827536900218	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	554	0.18379608587324706	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	465	0.1542692778538987	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	464	0.15393751596604086	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	457	0.15161518275103594	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	433	0.1436528974424476	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	431	0.14298937366673192	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	406	0.13469532647028573	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	397	0.13170946947956513	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	391	0.12971889815241805	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	383	0.12706480304955528	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	378	0.12540599361026603	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	366	0.12142485095597189	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	363	0.12042956529239833	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	354	0.11744370830167772	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	342	0.11346256564738355	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	341	0.11313080375952571	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	335	0.11114023243237864	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	326	0.10815437544165801	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	313	0.103841470899506	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.317618878578467E-4	0.0	0.0
2	0.0	0.0	6.635237757156933E-4	0.0	0.0
3	0.0	0.0	6.635237757156933E-4	0.0	0.0
4	0.0	0.0	6.635237757156933E-4	0.0	0.0
5	0.0	0.0	6.635237757156933E-4	0.0	0.0
6	0.0	0.0	0.0013270475514313867	0.0	0.0
7	0.0	0.0	0.0033176188785784665	3.317618878578467E-4	0.0
8	0.0	0.0	0.0036493807664363135	6.635237757156933E-4	0.0
9	0.0	0.0	0.004312904542152007	9.9528566357354E-4	0.0
10	0.0	0.0	0.004644666430009854	9.9528566357354E-4	0.0
11	0.0	0.0	0.00796228530858832	9.9528566357354E-4	0.0
12	0.0	0.0	0.008625809084304013	9.9528566357354E-4	0.0
13	0.0	0.0	0.009289332860019707	0.0013270475514313867	0.0
14	0.0	0.0	0.0099528566357354	0.0013270475514313867	0.0
15	0.0	0.0	0.010284618523593247	0.0013270475514313867	0.0
16	0.0	0.0	0.011279904187166786	0.0013270475514313867	0.0
17	0.0	0.0	0.011611666075024634	0.0013270475514313867	0.0
18	0.0	0.0	0.011611666075024634	0.0013270475514313867	0.0
19	0.0	0.0	0.01194342796288248	0.0013270475514313867	0.0
20	0.0	0.0	0.01293871362645602	0.0013270475514313867	0.0
21	0.0	0.0	0.013270475514313866	0.0016588094392892333	0.0
22	0.0	0.0	0.013602237402171714	0.0016588094392892333	0.0
23	0.0	0.0	0.013602237402171714	0.0016588094392892333	0.0
24	0.0	0.0	0.013602237402171714	0.00199057132714708	0.0
25	0.0	0.0	0.013602237402171714	0.00199057132714708	0.0
26	0.0	0.0	0.013602237402171714	0.00199057132714708	0.0
27	0.0	0.0	0.013602237402171714	0.004644666430009854	0.0
28	0.0	0.0	0.013602237402171714	0.011279904187166786	0.0
29	0.0	3.317618878578467E-4	0.013602237402171714	0.0199057132714708	0.0
30	0.0	3.317618878578467E-4	0.013602237402171714	0.038816140879368057	0.0
31	0.0	3.317618878578467E-4	0.013602237402171714	0.09322509048805491	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGCTC	25	0.0054899915	29.6	27
TTCGCCG	25	0.0054899915	29.6	24
GGTATCA	170	0.0	26.117647	1
GTATCAA	450	0.0	24.666666	1
CCAGGAC	155	0.0	22.677418	3
TTAGGAC	110	7.2759576E-11	21.863636	3
ATTATAC	80	6.925111E-7	20.8125	3
TAGGACC	80	6.925111E-7	20.8125	4
TTTAGGA	90	9.420182E-8	20.555555	2
ACCTAAG	45	0.0038193609	20.555555	1
AGGACAG	190	0.0	20.447369	5
GCATCAG	140	3.6379788E-12	19.821428	15
TCCAGGA	150	0.0	19.733335	2
ATCAAGC	150	1.2732926E-11	18.500002	30
TCGGGGG	60	9.2152803E-4	18.5	33
TTATACC	50	0.00702314	18.5	4
CACCTAG	50	0.00702314	18.5	27
CGGGGGT	50	0.00702314	18.5	34
CGCTCTC	50	0.00702314	18.5	29
GAGACCG	50	0.00702314	18.5	21
>>END_MODULE