FastQCFastQC Report
Fri 10 Feb 2017
ERR1632924.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632924.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420946
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT106592.532153767941731No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT82211.9529820927149801No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42191.0022663239465395No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22280.5292840411834296No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG18220.43283461536634155No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG17890.4249951300166767No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17760.42190684790923305No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA17600.4181058853154561No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA13980.33210910663125437No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG13050.31001601155492625No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT12880.30597748879903836No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12350.29338680020715246No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC10540.2503884108650516No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT10030.2382728425973878No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9700.23043335724772296No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9610.22829531578872347No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9600.22805775562661246No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC9510.22591971416761292No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG9180.21808022881794814No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9050.21499194671050445No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC8960.21285390525150497No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA8810.2092905028198391No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC8430.20026321665961908No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8360.19860029552484165No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC8320.19765005487639742No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA8260.19622469390373115No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC8170.19408665244473164No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7890.18743496790562209No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7860.18672228741928892No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA7820.18577204677084472No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7690.182683764663401No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA7580.1800706028801794No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT7580.1800706028801794No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7550.17935792239384624No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT7520.17864524190751307No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7500.17817012158329096No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7070.16795503461251562No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT7040.16724235412618246No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA6940.1648667525050719No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6830.1622535907218503No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT6570.1560770265069629No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6540.15536434602062973No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC6470.15370142488585234No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6440.15298874439951918No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6420.1525136240752971No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC6390.1518009435889639No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6380.15156338342685285No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6370.1513258232647418No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6330.15037558261629758No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6300.14966290212996441No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC6020.1430112175908549No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5920.14063561596974433No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5860.139210254997078No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5640.1339839314306348No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5380.12780736721574737No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT5360.12733224689152528No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5360.12733224689152528No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA5320.12638200624308107No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5310.12614444608097No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5260.12495664527041472No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG5260.12495664527041472No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA5120.12163080300085997No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5110.12139324283874892No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5020.11925520137974942No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT5010.11901764121763837No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4950.11759228024497204No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4870.1156917989480836No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC4850.11521667862386148No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4830.11474155829963939No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA4730.11236595667852883No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4720.11212839651641777No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA4650.11046547538164041No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4630.1099903550574183No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC4630.1099903550574183No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG4610.10951523473319619No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG4590.10904011440897407No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA4520.10737719327419669No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4450.10571427213941931No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4450.10571427213941931No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4420.10500159165308615No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC4340.1031011103561977No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG4320.10262599003197559No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4310.10238842986986453No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA4300.1021508697077535No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4240.10072550873508716No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4230.1004879485729761No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT4220.10025038841086505No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG4210.100012828248754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCAA507.3086994E-933.3000031
GTATTAT303.595637E-430.8333321
ATTATAG303.595637E-430.8333323
GGGTAAA250.00549243529.618
GGGAGGT250.00549243529.618
GCTCTGT250.00549243529.635
ACGTGCG250.00549243529.611
TCGTATG250.00549243529.637
TAAGTTC454.000325E-628.77777930
AGTTCCG454.000325E-628.77777932
CGTTACG405.9313246E-527.7516
TATCGTT405.9313246E-527.7513
GTTATCG405.9313246E-527.7511
GATATGT604.309186E-827.7499981
TGTAGGT604.309186E-827.7499985
TCGTTGC358.8600203E-426.4285716
TGCTCGT358.8600203E-426.4285721
GCTCGTT358.8600203E-426.4285714
CATTTTG358.8600203E-426.4285736
GTTGCGC358.8600203E-426.428579
Produced by FastQC (version 0.11.5)