FastQCFastQC Report
Fri 10 Feb 2017
ERR1632923.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632923.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300047
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28150.9381863508050406No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22430.7475495505704106No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12200.4066029655353995No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6330.21096694851139985No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5970.1989688282169127No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5690.18963695687675597No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4840.16130806173699452No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4520.15064306591967258No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG4090.1363119777901462No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3720.1239805763763677No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3560.11864807846770672No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3480.11598182951337624No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG3300.10998276936613263No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT3040.10131746026455855No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGG250.00548995229.59999822
GAGTCGT250.00548995229.59999819
GTCGTTG250.00548995229.59999821
CGGAGTC250.00548995229.59999817
CGTTAGA405.9257287E-527.7500021
GCCGGCA1450.022.96551715
GTATCAA14100.022.960991
GGTATCA5350.022.822431
AGGACCG603.7144826E-521.5833345
CGGCAGC1500.020.96666717
CCGCTCT1450.020.41379428
ACGGGTC1001.2802047E-820.34999815
CCGGCAG1550.020.29032316
GACCGTT555.1309256E-420.1818187
GCCGCTC1500.019.73333427
CGGGTCA851.2390428E-619.58823416
CGCCGGC1750.019.0285714
AACGTCA701.21538986E-418.528
GTTCTAC902.141449E-618.525
GGGTCAG902.141449E-618.517
Produced by FastQC (version 0.11.5)