FastQCFastQC Report
Fri 10 Feb 2017
ERR1632922.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632922.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174616
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3100.177532413982682No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.1362990791221881No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC1830.10480139277042197No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA1750.10021991111925597No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC200.001837663937.036
CGAGTTC250.00548374429.59999814
CCGAGTT250.00548374429.59999813
TAAGGTG250.00548374429.5999985
GCTCTAC358.8391773E-426.428571
GGTATCA1103.6379788E-1223.5454541
TTAGCCA400.001924669423.1250024
AATGGGT400.001924669423.1250025
ACCCTCG400.001924669423.12500224
TAACCCC400.001924669423.12500219
CTTAGCC400.001924669423.1250023
TAATATA603.703041E-521.5833324
AGCAAGG450.003813021620.55555310
AATATAG450.003813021620.5555535
TAGCAAG450.003813021620.5555539
AACCCCT555.119124E-420.18181820
TCGTGGA555.119124E-420.18181828
AAGTAAG609.194118E-418.57
CCCTCGT609.194118E-418.525
GTATTAG1205.0858944E-918.51
Produced by FastQC (version 0.11.5)