FastQCFastQC Report
Fri 10 Feb 2017
ERR1632921.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632921.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398748
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10340.2593116454502593No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7740.19410755665232177No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT6080.1524772538044078No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC6020.15097254406291694No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG5910.14821390953685035No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC5550.13918565108790515No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC4790.12012599436235416No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA4750.1191228545346936No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA4640.11636422000862699No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT4330.10858988634425754No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4160.10432654207670007No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC4100.10282183233520921No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT200.001840494637.017
GTATCAA7100.023.7112671
GGTATCA2450.023.4081651
CGGAGTC502.697815E-422.217
CTATCTC603.7184338E-521.5833343
CGGCCTT909.439282E-820.55555524
GGACCAT450.00382149220.55555526
GTATTAT555.135002E-420.1818181
CGAACTA656.887691E-519.92307724
GGGGGGA656.887691E-519.92307722
TTGTGCT500.00702702218.54
GCGAACT701.2166645E-418.523
GTCGTTG609.2223985E-418.521
ACGTCAA500.00702702218.529
CGGTACC609.2223985E-418.514
AGTCGTT609.2223985E-418.520
TACAACC500.00702702218.54
GATTATC1156.373739E-817.69565224
GTTAGCC752.0630032E-417.2666663
TGGGGGG752.0630032E-417.26666621
Produced by FastQC (version 0.11.5)