##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632921.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	398748
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.501966154062213	33.0	33.0	33.0	27.0	33.0
2	31.071160231524672	33.0	33.0	33.0	27.0	33.0
3	31.53136567456138	33.0	33.0	33.0	27.0	33.0
4	31.672582683800297	33.0	33.0	33.0	27.0	33.0
5	31.783151263454613	33.0	33.0	33.0	33.0	33.0
6	34.62694483734088	37.0	37.0	37.0	27.0	37.0
7	34.82397403874126	37.0	37.0	37.0	27.0	37.0
8	34.973113344769125	37.0	37.0	37.0	27.0	37.0
9	35.07872892152437	37.0	37.0	37.0	33.0	37.0
10	35.11213347778547	37.0	37.0	37.0	33.0	37.0
11	35.15353055062345	37.0	37.0	37.0	33.0	37.0
12	35.101028719893264	37.0	37.0	37.0	33.0	37.0
13	35.1202764653365	37.0	37.0	37.0	33.0	37.0
14	35.0847427447912	37.0	37.0	37.0	33.0	37.0
15	35.10843690752054	37.0	37.0	37.0	33.0	37.0
16	35.10080802913118	37.0	37.0	37.0	33.0	37.0
17	35.071207880666485	37.0	37.0	37.0	33.0	37.0
18	35.0792630934826	37.0	37.0	37.0	33.0	37.0
19	35.086924573916356	37.0	37.0	37.0	33.0	37.0
20	35.07613078937073	37.0	37.0	37.0	33.0	37.0
21	35.078869361100246	37.0	37.0	37.0	33.0	37.0
22	35.07402419573264	37.0	37.0	37.0	33.0	37.0
23	35.07391134250203	37.0	37.0	37.0	33.0	37.0
24	35.06437148274098	37.0	37.0	37.0	33.0	37.0
25	35.059752023834605	37.0	37.0	37.0	27.0	37.0
26	35.00760630774324	37.0	37.0	37.0	27.0	37.0
27	34.96210890085969	37.0	37.0	37.0	27.0	37.0
28	34.929795259161175	37.0	37.0	37.0	27.0	37.0
29	34.96829576574679	37.0	37.0	37.0	27.0	37.0
30	34.940631677149476	37.0	37.0	37.0	27.0	37.0
31	34.92320964619258	37.0	37.0	37.0	27.0	37.0
32	34.937712540250985	37.0	37.0	37.0	27.0	37.0
33	34.89983899605766	37.0	37.0	37.0	27.0	37.0
34	34.8324580938337	37.0	37.0	37.0	27.0	37.0
35	34.84909516787545	37.0	37.0	37.0	27.0	37.0
36	34.80694072446758	37.0	37.0	37.0	27.0	37.0
37	34.846479480774825	37.0	37.0	37.0	27.0	37.0
38	34.711742754822595	37.0	37.0	37.0	27.0	37.0
39	34.76756999408148	37.0	37.0	37.0	27.0	37.0
40	34.8030535576354	37.0	37.0	37.0	27.0	37.0
41	34.81988624394354	37.0	37.0	37.0	27.0	37.0
42	34.735000551726905	37.0	37.0	37.0	27.0	37.0
43	34.503325408528696	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	12.0
16	30.0
17	18.0
18	18.0
19	19.0
20	43.0
21	116.0
22	304.0
23	713.0
24	1400.0
25	2488.0
26	3947.0
27	5594.0
28	8005.0
29	10494.0
30	13398.0
31	16720.0
32	21176.0
33	27705.0
34	38542.0
35	68800.0
36	179204.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.79493815642962	20.53853561647958	13.691604722782309	24.974921504308487
2	15.599325890035812	23.747830710122685	38.28006660848456	22.372776791356948
3	17.861406201410414	26.928787103634377	31.09507759286567	24.11472910208954
4	13.000190596567254	18.420155085417356	38.43906427116876	30.140590046846633
5	13.52307723173533	37.71078475628718	35.692467423034095	13.073670588943392
6	29.292686107516523	39.69349062565831	17.683599666957576	13.330223599867585
7	25.81279404536198	32.597279484787386	23.977549730656953	17.612376739193678
8	23.800746336031782	36.5817508802552	21.212896365624403	18.40460641808862
9	25.620441983408064	16.08183614714055	19.93640093492632	38.36132093452506
10	14.329350868217523	28.942590307662986	34.29534442805983	22.432714396059666
11	33.14449226077623	24.0941647356225	24.40563965211111	18.355703351490163
12	22.164876062074292	25.866963596055655	30.70034207068123	21.267818271188823
13	27.315748292154446	22.391084093211752	27.213428029733066	23.07973958490074
14	21.823306950755867	21.36863382386871	27.707975964769727	29.100083260605697
15	23.58883304743848	28.904721779168796	24.955360277669104	22.551084895723612
16	22.956604171055403	29.03512995676467	24.982695837972855	23.025570034207067
17	22.220048752595627	28.156881037647835	26.447029201400383	23.176041008356155
18	22.061803444782168	28.02296187065515	27.812302506846432	22.10293217771625
19	24.334416724347207	26.87035420867315	27.83286687331347	20.962362193666177
20	24.057800916869805	26.47612025640254	27.96151955621094	21.50455927051672
21	22.46782429002779	27.922146317975262	26.82370820668693	22.786321185310022
22	22.038731228745974	27.69142415761333	27.132675273606388	23.13716934003431
23	22.215534623371152	27.746346063177747	27.481266363718436	22.556852949732665
24	23.268329872500928	27.80603288292355	26.52577567787174	22.399861566703784
25	23.132404425852922	27.11411718679467	27.359635659614597	22.39384272773782
26	23.04914382015709	27.298193345170386	27.195873082749006	22.45678975192352
27	22.75672856039403	27.033364430667987	27.014555558899357	23.195351450038622
28	21.965752806283668	27.512614483332833	27.732552890547414	22.789079819836086
29	23.138674049775798	27.719010502874	26.89392799462317	22.248387452727037
30	22.376538565710675	27.973557234142866	27.195873082749006	22.454031117397456
31	22.97165126847031	27.273867204349617	27.395247123496546	22.35923440368353
32	21.970517720465054	27.298945700041127	27.586345260665883	23.144191318827932
33	22.337416112431914	27.094555960155287	27.790735000551724	22.777292926861076
34	22.29403031488559	27.11336483192392	27.553241646353083	23.0393632068374
35	22.578922025941196	27.709982244425053	27.296939420385808	22.414156309247947
36	22.719863171727507	27.672615285844692	27.035621495280225	22.571900047147572
37	23.287640314183395	26.67599586706391	27.122393090372864	22.91397072837983
38	23.336292595824933	27.185089329601652	27.02834873152969	22.45026934304373
39	22.62055232878911	27.313992797456038	27.54922908704244	22.51622578671241
40	22.56537963826778	27.222957858095842	27.488789912425894	22.72287259121049
41	22.12625517870936	27.07374080873133	27.945218534011456	22.854785478547853
42	22.830710122684	26.962643072817922	27.28740959202303	22.919237212475046
43	22.559360799301814	26.433988383640795	27.741079579082527	23.26557123797486
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11.0
1	15.0
2	19.0
3	64.0
4	109.0
5	109.0
6	156.0
7	203.0
8	190.0
9	177.0
10	255.5
11	334.0
12	334.0
13	489.0
14	644.0
15	1187.0
16	1730.0
17	1971.5
18	2213.0
19	2213.0
20	2310.5
21	2408.0
22	2978.5
23	3549.0
24	4647.0
25	5745.0
26	5745.0
27	7254.0
28	8763.0
29	11709.5
30	14656.0
31	16122.0
32	17588.0
33	17588.0
34	19520.0
35	21452.0
36	23165.0
37	24878.0
38	27248.0
39	29618.0
40	29618.0
41	31390.5
42	33163.0
43	31381.5
44	29600.0
45	29966.5
46	30333.0
47	30333.0
48	29778.5
49	29224.0
50	29965.5
51	30707.0
52	31106.5
53	31506.0
54	31506.0
55	27084.0
56	22662.0
57	19937.0
58	17212.0
59	14796.0
60	12380.0
61	12380.0
62	10897.5
63	9415.0
64	7885.0
65	6355.0
66	5318.0
67	4281.0
68	4281.0
69	3622.5
70	2964.0
71	2479.5
72	1995.0
73	1644.0
74	1293.0
75	1293.0
76	977.5
77	662.0
78	527.5
79	393.0
80	302.0
81	211.0
82	211.0
83	165.5
84	120.0
85	109.0
86	98.0
87	76.0
88	54.0
89	54.0
90	36.0
91	18.0
92	11.5
93	5.0
94	2.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	398748.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.02068475715098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.28022090049078	55.992411202752926
2	5.250441818986949	6.5127199378230385
3	1.3522763791077184	2.5160732103954415
4	0.6903036123333517	1.7125241090899745
5	0.41954250459452813	1.3010156709841398
6	0.2827336714751963	1.0521201545277021
7	0.2120915204269	0.9207842929643127
8	0.17310080669260444	0.858866445047244
9	0.12150791940856269	0.6782403928622197
>10	0.988233678797902	12.59574146409221
>50	0.14407749014326363	6.303262066831267
>100	0.08302770618383783	8.505150457691876
>500	0.0020349927986234757	0.7900849092590839
>1k	4.069985597246952E-4	0.26100568567856
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1034	0.2593116454502593	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	774	0.19410755665232177	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	608	0.1524772538044078	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	602	0.15097254406291694	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	591	0.14821390953685035	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	555	0.13918565108790515	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	479	0.12012599436235416	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	475	0.1191228545346936	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	464	0.11636422000862699	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	433	0.10858988634425754	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	416	0.10432654207670007	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	410	0.10282183233520921	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.507849569151444E-4	0.0	0.0
2	0.0	0.0	2.507849569151444E-4	0.0	0.0
3	0.0	0.0	2.507849569151444E-4	0.0	0.0
4	0.0	0.0	0.0010031398276605776	2.507849569151444E-4	0.0
5	0.0	0.0	0.0012539247845757221	2.507849569151444E-4	0.0
6	0.0	0.0	0.0022570646122362995	2.507849569151444E-4	0.0
7	0.0	0.0	0.003761774353727166	2.507849569151444E-4	0.0
8	0.0	0.0	0.004764914181387744	2.507849569151444E-4	0.0
9	0.0	0.0	0.006771193836708899	0.0012539247845757221	0.0
10	2.507849569151444E-4	0.0	0.0077743336643694766	0.0012539247845757221	0.0
11	2.507849569151444E-4	0.0	0.012790032802672364	0.0012539247845757221	0.0
12	2.507849569151444E-4	0.0	0.01479631245799352	0.0012539247845757221	0.0
13	2.507849569151444E-4	0.0	0.015548667328738953	0.0012539247845757221	0.0
14	2.507849569151444E-4	0.0	0.01655180715639953	0.0012539247845757221	0.0
15	2.507849569151444E-4	0.0	0.018558086811720685	0.0012539247845757221	0.0
16	2.507849569151444E-4	0.0	0.02056436646704184	0.0015047097414908664	0.0
17	2.507849569151444E-4	0.0	0.02106593638087213	0.0017554946984060108	0.0
18	2.507849569151444E-4	0.0	0.02156750629470242	0.002006279655321155	0.0
19	2.507849569151444E-4	0.0	0.021818291251617564	0.002006279655321155	0.0
20	2.507849569151444E-4	0.0	0.021818291251617564	0.0022570646122362995	0.0
21	2.507849569151444E-4	0.0	0.02206907620853271	0.0027586345260665886	0.0
22	2.507849569151444E-4	0.0	0.022319861165447853	0.003009419482981733	0.0
23	2.507849569151444E-4	0.0	0.022570646122362995	0.0032602044398968772	0.0
24	2.507849569151444E-4	0.0	0.022570646122362995	0.0035109893968120216	0.0
25	2.507849569151444E-4	0.0	0.02282143107927814	0.003761774353727166	0.0
26	2.507849569151444E-4	0.0	0.02282143107927814	0.00401255931064231	0.0
27	2.507849569151444E-4	0.0	0.02282143107927814	0.006018838965963466	0.0
28	2.507849569151444E-4	0.0	0.02282143107927814	0.011034538104266354	0.0
29	2.507849569151444E-4	0.0	0.02282143107927814	0.024827710734599297	0.0
30	2.507849569151444E-4	0.0	0.02282143107927814	0.046395217029301716	0.0
31	2.507849569151444E-4	0.0	0.02282143107927814	0.11185009078415441	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	20	0.0018404946	37.0	17
GTATCAA	710	0.0	23.711267	1
GGTATCA	245	0.0	23.408165	1
CGGAGTC	50	2.697815E-4	22.2	17
CTATCTC	60	3.7184338E-5	21.583334	3
CGGCCTT	90	9.439282E-8	20.555555	24
GGACCAT	45	0.003821492	20.555555	26
GTATTAT	55	5.135002E-4	20.181818	1
CGAACTA	65	6.887691E-5	19.923077	24
GGGGGGA	65	6.887691E-5	19.923077	22
TTGTGCT	50	0.007027022	18.5	4
GCGAACT	70	1.2166645E-4	18.5	23
GTCGTTG	60	9.2223985E-4	18.5	21
ACGTCAA	50	0.007027022	18.5	29
CGGTACC	60	9.2223985E-4	18.5	14
AGTCGTT	60	9.2223985E-4	18.5	20
TACAACC	50	0.007027022	18.5	4
GATTATC	115	6.373739E-8	17.695652	24
GTTAGCC	75	2.0630032E-4	17.266666	3
TGGGGGG	75	2.0630032E-4	17.266666	21
>>END_MODULE