FastQCFastQC Report
Fri 10 Feb 2017
ERR1632920.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632920.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417205
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10050.24088877170695464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7520.1802471207200297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC1050.026.4285736
TTAACGG1050.026.4285735
AACGGCC1150.025.7391337
TTCTGCG551.8988949E-523.54545418
CTATGCT400.001929136723.1254
GTTTAAC1250.022.19999933
GGTATCA2450.021.1428591
TATTAGA1700.020.6764722
CGGGTAA450.003821785620.55555523
GCGGGTA450.003821785620.55555522
ATTAGAG1550.020.2903233
TTTAACG1403.6379788E-1219.82142834
GCACCGC1355.4569682E-1119.18518610
GGCACCG1409.276846E-1118.59
ACGGGCT801.6126512E-518.516
GTCAACC701.2168367E-418.515
TAGGACC609.2233764E-418.54
CGAACCT1002.8640352E-718.49999827
GGTAACG500.007027557618.49999825
GAATCAA500.007027557618.4999981
Produced by FastQC (version 0.11.5)