##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632920.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	417205
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.393413310003474	33.0	33.0	33.0	27.0	33.0
2	30.994156349995805	33.0	33.0	33.0	27.0	33.0
3	31.442264594144365	33.0	33.0	33.0	27.0	33.0
4	31.60975060222193	33.0	33.0	33.0	27.0	33.0
5	31.726899246173943	33.0	33.0	33.0	27.0	33.0
6	34.548032741697725	37.0	37.0	37.0	27.0	37.0
7	34.74872304981963	37.0	37.0	37.0	27.0	37.0
8	34.9017988758524	37.0	37.0	37.0	27.0	37.0
9	34.99422825709184	37.0	37.0	37.0	27.0	37.0
10	35.01316379238025	37.0	37.0	37.0	27.0	37.0
11	35.06723553169305	37.0	37.0	37.0	33.0	37.0
12	35.02999964046452	37.0	37.0	37.0	27.0	37.0
13	35.04790930118287	37.0	37.0	37.0	27.0	37.0
14	35.02981028511164	37.0	37.0	37.0	27.0	37.0
15	35.044925156697545	37.0	37.0	37.0	27.0	37.0
16	35.04588871178437	37.0	37.0	37.0	27.0	37.0
17	35.02971680588679	37.0	37.0	37.0	27.0	37.0
18	35.013305209669106	37.0	37.0	37.0	27.0	37.0
19	35.00577893361777	37.0	37.0	37.0	27.0	37.0
20	35.00894763964957	37.0	37.0	37.0	27.0	37.0
21	35.018904375546796	37.0	37.0	37.0	27.0	37.0
22	35.015503169904484	37.0	37.0	37.0	27.0	37.0
23	35.00234417133064	37.0	37.0	37.0	27.0	37.0
24	34.997054205965895	37.0	37.0	37.0	27.0	37.0
25	34.99150058124903	37.0	37.0	37.0	27.0	37.0
26	34.95169281288575	37.0	37.0	37.0	27.0	37.0
27	34.88421279706619	37.0	37.0	37.0	27.0	37.0
28	34.86838844213276	37.0	37.0	37.0	27.0	37.0
29	34.874579643101114	37.0	37.0	37.0	27.0	37.0
30	34.83924209920782	37.0	37.0	37.0	27.0	37.0
31	34.83138984432114	37.0	37.0	37.0	27.0	37.0
32	34.86426337172373	37.0	37.0	37.0	27.0	37.0
33	34.85161491353172	37.0	37.0	37.0	27.0	37.0
34	34.78591579679055	37.0	37.0	37.0	27.0	37.0
35	34.77694658501216	37.0	37.0	37.0	27.0	37.0
36	34.769628839539315	37.0	37.0	37.0	27.0	37.0
37	34.795071967018615	37.0	37.0	37.0	27.0	37.0
38	34.66137989717285	37.0	37.0	37.0	27.0	37.0
39	34.72642945314654	37.0	37.0	37.0	27.0	37.0
40	34.75565010007071	37.0	37.0	37.0	27.0	37.0
41	34.79365060342038	37.0	37.0	37.0	27.0	37.0
42	34.702604235327954	37.0	37.0	37.0	27.0	37.0
43	34.47195503409595	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	13.0
16	16.0
17	20.0
18	18.0
19	18.0
20	46.0
21	116.0
22	348.0
23	783.0
24	1579.0
25	2759.0
26	4389.0
27	6213.0
28	8555.0
29	11293.0
30	14585.0
31	18258.0
32	22916.0
33	29593.0
34	41234.0
35	72398.0
36	182054.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.85881041694131	20.42017713114656	13.291307630541342	25.42970482137079
2	16.14625903332894	23.167986960846587	37.34423125322084	23.341522752603634
3	17.978212149902326	26.812957658704956	30.07897796047507	25.129852230917653
4	12.972040124159587	17.995230162629884	38.744741793602664	30.28798791960787
5	14.216991646792343	36.954015412087585	35.20595390755145	13.623039033568629
6	29.979746167951006	39.646936158483236	17.312112750326577	13.061204923239176
7	26.833331335914	31.829196677892163	23.724068503493488	17.613403482700352
8	24.551958869141068	36.0065195767069	20.571901103773925	18.86962045037811
9	25.930897280713317	15.39435049915509	20.119845160053213	38.55490706007838
10	14.913052336381396	28.119509593605063	33.998394074855284	22.969043995158255
11	34.26636785273427	23.440035474167374	24.06682566124567	18.226771011852687
12	23.047422729833055	25.420836279526853	30.4085521506214	21.123188840018695
13	28.03921333636941	22.025143514579163	26.04355173116334	23.89209141788809
14	21.81972891024796	20.79984659819513	27.608729521458276	29.771694970098633
15	23.84319459258638	28.620462362627485	24.203449143706333	23.332893901079803
16	23.15768027708201	28.1432389352956	25.156218166129364	23.54286262149303
17	22.61046727627905	27.4342349684208	26.38487074699488	23.57042700830527
18	23.161994702843923	27.413381910571545	27.347467072542276	22.077156314042256
19	24.587912417157032	26.630553325103968	27.042101604726692	21.739432653012308
20	24.14065027983845	26.44191704318021	27.130307642525857	22.28712503445548
21	23.292625927301927	27.098908210591915	27.04066346280605	22.567802399300106
22	22.91631212473484	26.967557915173597	27.0646324948167	23.051497465274863
23	23.112618496902	26.699823827614722	27.43303651682027	22.754521158663007
24	23.294064069222564	27.098428829951704	26.21037619395741	23.397130906868327
25	23.31563619803214	26.90691626418667	27.072062894739997	22.70538464304119
26	23.45321844177323	27.079013914023083	26.88031063865486	22.58745700554883
27	23.25763114056639	27.141093706930647	26.546901403386826	23.05437374911614
28	22.90169101520835	26.806965400702293	27.30959600196546	22.981747582123894
29	23.91606044989873	26.95797030236934	26.374803753550413	22.751165494181517
30	23.59175944679474	26.68592178904855	26.984336237581047	22.737982526575664
31	23.359499526611618	26.575664241799597	27.098908210591915	22.965928020996873
32	22.407209884828802	26.664109969918865	27.238407976893853	23.69027216835848
33	22.65313215325799	26.716362459702065	27.687587636773287	22.942917750266655
34	23.149291115878285	26.269819393343802	27.427283949137717	23.1536055416402
35	23.148092664277755	27.08189019786436	26.90020493522369	22.869812202634197
36	23.702975755324122	26.802171594300162	26.58069773852183	22.914154911853885
37	23.043347994391247	26.556249325870972	26.8072050910224	23.59319758871538
38	23.21999976030968	26.81487518126581	26.78946800733452	23.175657051089992
39	22.925899737539098	26.77508658812814	27.063913423856377	23.235100250476385
40	22.96664709195719	26.98385685694083	27.339317601658657	22.71017844944332
41	23.004278472213898	26.204623626274852	27.39133040112175	23.399767500389494
42	22.7243201783296	26.972351721575727	27.2177946093647	23.085533490729976
43	23.287113049939478	25.899737539099483	27.44645917474623	23.36669023621481
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	7.5
2	14.0
3	60.5
4	107.0
5	107.0
6	147.5
7	188.0
8	173.5
9	159.0
10	210.5
11	262.0
12	262.0
13	426.5
14	591.0
15	1045.5
16	1500.0
17	1741.5
18	1983.0
19	1983.0
20	1970.5
21	1958.0
22	2363.0
23	2768.0
24	3767.5
25	4767.0
26	4767.0
27	6417.0
28	8067.0
29	9915.5
30	11764.0
31	14258.5
32	16753.0
33	16753.0
34	19432.0
35	22111.0
36	24435.0
37	26759.0
38	28653.5
39	30548.0
40	30548.0
41	31854.0
42	33160.0
43	33894.0
44	34628.0
45	33794.5
46	32961.0
47	32961.0
48	32092.5
49	31224.0
50	30977.5
51	30731.0
52	28959.0
53	27187.0
54	27187.0
55	26250.5
56	25314.0
57	22211.0
58	19108.0
59	17608.5
60	16109.0
61	16109.0
62	14194.0
63	12279.0
64	10357.0
65	8435.0
66	7109.5
67	5784.0
68	5784.0
69	4792.5
70	3801.0
71	3186.0
72	2571.0
73	2074.0
74	1577.0
75	1577.0
76	1222.5
77	868.0
78	689.5
79	511.0
80	386.5
81	262.0
82	262.0
83	222.5
84	183.0
85	152.5
86	122.0
87	86.5
88	51.0
89	51.0
90	42.0
91	33.0
92	18.5
93	4.0
94	3.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	417205.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.74064472508587
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.00252909976645	64.56839464505548
2	5.91419440179297	8.485762388282422
3	1.558028794080586	3.3532197056276782
4	0.6974732832374209	2.001487320719
5	0.3946424267985866	1.415595106720155
6	0.2643387035216551	1.137829740986212
7	0.17188337753152	0.8631717025145609
8	0.1425858027987934	0.8183357937143513
9	0.10654639304385378	0.6879336237088627
>10	0.6446957630409579	8.749661261050232
>50	0.06984054241521911	3.458384246522102
>100	0.03256986829583678	4.036989728375121
>500	3.3577183810141017E-4	0.1811454308573358
>1k	3.3577183810141017E-4	0.24208930586651928
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1005	0.24088877170695464	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	752	0.1802471207200297	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.396903201064225E-4	0.0	0.0
2	0.0	0.0	2.396903201064225E-4	0.0	0.0
3	0.0	0.0	2.396903201064225E-4	0.0	0.0
4	0.0	0.0	2.396903201064225E-4	0.0	0.0
5	0.0	0.0	9.5876128042569E-4	0.0	0.0
6	0.0	0.0	0.0011984516005321124	0.0	0.0
7	0.0	0.0	0.0043144257619156055	4.79380640212845E-4	0.0
8	4.79380640212845E-4	0.0	0.005273187042341295	4.79380640212845E-4	0.0
9	4.79380640212845E-4	0.0	0.005992258002660562	9.5876128042569E-4	0.0
10	0.00191752256085138	0.0	0.00671132896297983	0.0011984516005321124	0.0
11	0.00191752256085138	0.0	0.011025754724895436	0.0011984516005321124	0.0
12	0.00191752256085138	0.0	0.011984516005321124	0.0011984516005321124	0.0
13	0.00191752256085138	0.0	0.013182967605853238	0.0011984516005321124	0.0
14	0.00191752256085138	0.0	0.013902038566172505	0.0011984516005321124	0.0
15	0.00191752256085138	0.0	0.01534018048681104	0.0011984516005321124	0.0
16	0.00191752256085138	0.0	0.017257703047662422	0.0011984516005321124	0.0
17	0.00191752256085138	0.0	0.017257703047662422	0.0011984516005321124	0.0
18	0.00191752256085138	0.0	0.017737083687875264	0.0011984516005321124	0.0
19	0.00191752256085138	0.0	0.01821646432808811	0.0011984516005321124	0.0
20	0.00191752256085138	0.0	0.01845615464819453	0.0011984516005321124	0.0
21	0.00191752256085138	0.0	0.018695844968300956	0.0016778322407449576	0.0
22	0.00191752256085138	0.0	0.0191752256085138	0.0016778322407449576	0.0
23	0.00191752256085138	0.0	0.019894296568833066	0.002396903201064225	0.0
24	0.00191752256085138	0.0	0.019894296568833066	0.00287628384127707	0.0
25	0.00191752256085138	0.0	0.019894296568833066	0.00287628384127707	0.0
26	0.00191752256085138	0.0	0.019894296568833066	0.0031159741613834926	0.0
27	0.00191752256085138	0.0	0.019894296568833066	0.00479380640212845	0.0
28	0.00191752256085138	0.0	0.02013398688893949	0.011984516005321124	0.0
29	0.00191752256085138	0.0	0.020373677209045912	0.024448412650855095	0.0
30	0.00191752256085138	0.0	0.020373677209045912	0.04865713498160377	0.0
31	0.00191752256085138	0.0	0.020373677209045912	0.12320082453470117	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGGC	105	0.0	26.42857	36
TTAACGG	105	0.0	26.42857	35
AACGGCC	115	0.0	25.73913	37
TTCTGCG	55	1.8988949E-5	23.545454	18
CTATGCT	40	0.0019291367	23.125	4
GTTTAAC	125	0.0	22.199999	33
GGTATCA	245	0.0	21.142859	1
TATTAGA	170	0.0	20.676472	2
CGGGTAA	45	0.0038217856	20.555555	23
GCGGGTA	45	0.0038217856	20.555555	22
ATTAGAG	155	0.0	20.290323	3
TTTAACG	140	3.6379788E-12	19.821428	34
GCACCGC	135	5.4569682E-11	19.185186	10
GGCACCG	140	9.276846E-11	18.5	9
ACGGGCT	80	1.6126512E-5	18.5	16
GTCAACC	70	1.2168367E-4	18.5	15
TAGGACC	60	9.2233764E-4	18.5	4
CGAACCT	100	2.8640352E-7	18.499998	27
GGTAACG	50	0.0070275576	18.499998	25
GAATCAA	50	0.0070275576	18.499998	1
>>END_MODULE