FastQCFastQC Report
Fri 10 Feb 2017
ERR1632919.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632919.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539249
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43080.7988888250140473No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32370.6002792772911958No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16630.3083918560813279No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC15610.289476661060104No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG14860.27556842942685106No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA14510.269077921331333No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC14420.2674089335353427No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG14000.25962032382072103No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC12770.23681082394218628No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA12680.2351418361461959No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC12430.23050575893511163No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG12300.22809499878534778No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12200.22624056790091404No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG11940.22141904760138637No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT11800.21882284436317914No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT11750.2178956289209623No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG11550.21418676715209486No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10630.1971260030153046No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA10570.19601334448464436No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG10550.19564245830775762No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT10040.18618486079714566No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA9720.18025068196695776No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT9690.17969435270162765No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG9430.17487283240209997No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC9350.17338928769455297No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC9300.17246207225233612No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG8870.16448801944927113No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC8770.1626335885648374No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC8650.1604082715035169No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT8430.15632852355776275No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8330.154474092673329No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT8260.1531759910542254No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA8180.15169244634667842No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG8080.1498380154622447No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA8000.14835447075469774No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT7830.1452019382511604No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC7780.14427472280894355No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG7760.1439038366320568No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7520.13945320250941587No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7500.13908231633252913No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA7330.1359297838289918No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC7050.13073737735257737No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC6970.1292538326450304No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG6890.1277702879374834No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6890.1277702879374834No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6820.1264721863183798No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC6760.12535952778771958No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6700.12424686925705936No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG6650.12331965381484249No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA6650.12331965381484249No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6630.12294876763795574No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC6590.12220699528418227No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6540.1212797798419654No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA6470.11998167822286178No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT6460.11979623513441844No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC6440.11942534895753168No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT6430.11923990586908831No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6380.11831269042687144No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT6240.11571648718866423No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG6220.11534560101177749No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA6190.11478927174644736No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6070.11256395468512691No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT5930.1099677514469197No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA5910.10959686527003294No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5830.10811332056248597No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5760.10681521894338236No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC5750.106629775854939No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT5740.10644433276649563No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG5740.10644433276649563No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5710.10588800350116552No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5650.10477534497050527No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT5630.10440445879361852No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG5630.10440445879361852No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT5480.10162281246696794No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCAT5460.10125192629008122No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG5450.10106648320163784No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCG200.001841069737.021
CTCGAAT200.001841069737.033
GCTCGAA200.001841069737.032
GCTGGCC250.00549378829.618
GAGTCGT250.00549378829.611
AGCCCGC250.00549378829.618
TGAATCG250.00549378829.610
GGCTCGA250.00549378829.631
AATTTCT454.003068E-628.77777927
TCTATCG454.003068E-628.77777931
TCGGCGT405.9343714E-527.7511
CTAACTT405.9343714E-527.7522
CGCTCTC1350.027.40740629
CCGCTCT1300.027.03846228
GGTATCA9200.026.9456521
CGGGACG358.863262E-426.42857217
CCGGCAG1650.025.78787816
GCCGCTC1300.025.61538527
GGCAGCT1700.025.02941118
CGGCGTA451.3219393E-424.66666812
Produced by FastQC (version 0.11.5)