FastQCFastQC Report
Fri 10 Feb 2017
ERR1632918.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632918.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442653
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15260.3447395589773479No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10910.24646845271578416No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA9800.22139237732490236No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5540.12515446636530192No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA5300.1197326122267329No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC4850.10956663571691597No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG4700.1061779768803103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3100.027.4516141
TAGTACC551.8992341E-523.5454544
GTATCAA8400.022.6845251
CCGCTCT502.6984676E-422.19999928
AGTACCG855.1626557E-821.7647065
GTTCTAG450.00382214820.5555551
CCCTTAG450.00382214820.5555551
CGCAATA450.00382214820.55555528
AGCTTCG555.13623E-420.18181821
GCTTCGC555.13623E-420.18181822
ATAGTAC1403.6379788E-1219.8214283
GCTTTAT2450.018.877551
CTTTATT3050.018.8032782
CATCTAT609.224592E-418.51
CGGGGTT701.2170496E-418.516
CGCTCTC609.224592E-418.529
TCGCCGG500.007028219318.49999813
GTATTAG2000.018.4999981
CCGTCCG500.007028219318.49999828
ACGATTA500.007028219318.49999823
Produced by FastQC (version 0.11.5)