FastQCFastQC Report
Fri 10 Feb 2017
ERR1632917.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632917.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504139
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16190.3211415899186534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11230.22275602561991834No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC6210.12318031336595661No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6080.12060165946296557No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT6040.11980822749281447No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC5930.117626289574899No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG5500.10909689589577477No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC5250.10413794608233047No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA5230.10374123009725493No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC5170.10255108214202828No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCGT200.001840956237.015
TCTATCG200.001840956237.031
CGAGTCG751.4551915E-1129.621
GCCGTCC803.274181E-1127.7527
CCGTCCG754.6020432E-1027.13333328
AGCTTCG1300.027.03846221
CGGGTAT358.862461E-426.4285716
TCGCCGT856.91216E-1126.11764525
CGCCGTC856.91216E-1126.11764526
GGTATCA3750.025.6533321
GCTTCGC1300.025.61538522
GCGAGTC901.4188117E-1024.66666620
TTCGCCG1400.023.78571324
GTATCAA10650.023.2769951
CGGCATG400.001929691823.12525
TACACGC400.001929691823.12519
GCCGCTC1350.021.92592627
CGTCCGC855.1661118E-821.76470629
GCCGGCA1450.021.68965715
CCGGCAG1550.021.48387116
Produced by FastQC (version 0.11.5)