FastQCFastQC Report
Fri 10 Feb 2017
ERR1632916.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632916.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423346
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14370.33943866246521753No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11410.2695194946922848No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5140.12141368998407921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGT451.0772237E-732.888894
GTATCAA8200.025.7195131
AACGATT451.3212839E-424.66666622
GGTATCA2800.023.1251
CGGGATA502.6981963E-422.217
GTTGTAT705.0893686E-621.1428571
AGGTCGC555.1357213E-420.18181835
TCGTTTG609.2236785E-418.512
CAACGAT609.2236785E-418.521
TTCGTTT609.2236785E-418.511
ACCCTTC500.00702772118.57
ACGGGAT1103.8344297E-818.516
GCCGCTC701.2168899E-418.527
GTTCGTT609.2236785E-418.510
ACGTGAT609.2236785E-418.537
TATAGTG801.6127418E-518.55
GTATAGG801.6127418E-518.51
AAGATAG902.1456872E-618.55
GACGTGC953.595509E-617.52631632
TTCGCCG953.595509E-617.52631624
Produced by FastQC (version 0.11.5)