FastQCFastQC Report
Fri 10 Feb 2017
ERR1632915.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632915.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197966
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4460.22529121162219776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3440.17376721255165029No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC2150.10860450784478143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1050.029.952381
CCCGAAA250.005485493729.637
TACATCG400.001925575823.1255
GAATTAT400.001925575823.1251
GTATCAA3050.022.4426211
CTACAGT502.6911678E-422.24
TCTGTAC450.003814798320.5555533
TACTGAG450.003814798320.5555532
TAATACA500.007014828718.54
TGACAAT801.6056827E-518.533
AATACAG609.2000474E-418.55
AAAGCGT500.007014828718.535
TCGAAAC500.007014828718.516
CTGCGTC752.0563958E-417.2666669
ACAATTA752.0563958E-417.26666635
CGTCAGA752.0563958E-417.26666612
GTTCAGC650.001573695917.07692321
GTCTGGA700.002582342415.8571431
GGGTTCA957.0126014E-515.57894819
ACTGACA855.336346E-415.23529431
Produced by FastQC (version 0.11.5)