##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632915.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	197966
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.47096471111201	33.0	33.0	33.0	14.0	33.0
2	30.368467312568825	33.0	33.0	33.0	27.0	33.0
3	30.83083963912995	33.0	33.0	33.0	27.0	33.0
4	31.021746158431245	33.0	33.0	33.0	27.0	33.0
5	31.11521675439217	33.0	33.0	33.0	27.0	33.0
6	33.30834082620248	37.0	33.0	37.0	27.0	37.0
7	33.56256629926351	37.0	33.0	37.0	27.0	37.0
8	33.6723932392431	37.0	33.0	37.0	27.0	37.0
9	33.72720063041128	37.0	33.0	37.0	27.0	37.0
10	33.722103795601264	37.0	33.0	37.0	27.0	37.0
11	33.86847236394128	37.0	33.0	37.0	27.0	37.0
12	33.886020831860016	37.0	33.0	37.0	27.0	37.0
13	33.88421244052009	37.0	33.0	37.0	27.0	37.0
14	33.92619944839013	37.0	33.0	37.0	27.0	37.0
15	33.938201509350094	37.0	33.0	37.0	27.0	37.0
16	33.95766949880282	37.0	33.0	37.0	27.0	37.0
17	33.92254730610307	37.0	33.0	37.0	27.0	37.0
18	33.92220886414839	37.0	33.0	37.0	27.0	37.0
19	33.897664245375466	37.0	33.0	37.0	27.0	37.0
20	33.891117666670034	37.0	33.0	37.0	27.0	37.0
21	33.90508471151612	37.0	33.0	37.0	27.0	37.0
22	33.90461493387753	37.0	33.0	37.0	27.0	37.0
23	33.84595334552398	37.0	33.0	37.0	27.0	37.0
24	33.85368699675702	37.0	33.0	37.0	27.0	37.0
25	33.8368810805896	37.0	33.0	37.0	27.0	37.0
26	33.77622420011517	37.0	33.0	37.0	27.0	37.0
27	33.68848691189396	37.0	33.0	37.0	27.0	37.0
28	33.64183748724528	37.0	33.0	37.0	27.0	37.0
29	33.71391552084702	37.0	33.0	37.0	27.0	37.0
30	33.69112372831698	37.0	33.0	37.0	27.0	37.0
31	33.63168422860491	37.0	33.0	37.0	27.0	37.0
32	33.679631855975266	37.0	33.0	37.0	27.0	37.0
33	33.64653021225867	37.0	33.0	37.0	27.0	37.0
34	33.555176141357606	37.0	33.0	37.0	27.0	37.0
35	33.548139579523756	37.0	33.0	37.0	27.0	37.0
36	33.515164220118606	37.0	33.0	37.0	27.0	37.0
37	33.555827768404676	37.0	33.0	37.0	27.0	37.0
38	33.43757008779286	37.0	33.0	37.0	27.0	37.0
39	33.50570299950497	37.0	33.0	37.0	27.0	37.0
40	33.49947465726438	37.0	33.0	37.0	27.0	37.0
41	33.56121758281725	37.0	33.0	37.0	27.0	37.0
42	33.44926401503288	37.0	33.0	37.0	27.0	37.0
43	33.14221128880717	37.0	33.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	6.0
16	17.0
17	19.0
18	15.0
19	38.0
20	104.0
21	238.0
22	527.0
23	1066.0
24	1856.0
25	2766.0
26	4181.0
27	5473.0
28	7117.0
29	8552.0
30	10365.0
31	12443.0
32	15189.0
33	18610.0
34	24685.0
35	37507.0
36	47190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.485739975551354	21.663821060182052	12.866350787508965	24.984088176757624
2	16.813493226109532	24.40823171655739	35.33586575472556	23.44240930260752
3	18.68957295697241	27.312265742602264	29.700554640695877	24.29760665972945
4	14.068072295242617	19.677116272491237	37.27912873927846	28.975682692987686
5	15.395572977177899	36.7487346311993	34.646353414222645	13.20933897740016
6	30.10870553529394	39.85330814382267	17.0059505167554	13.03203580412798
7	26.850065162704706	32.370710121940135	23.12467797500581	17.654546740349353
8	24.539567400462705	36.28198781608963	20.390370063546264	18.788074719901395
9	25.87060404311852	15.479425759978987	20.14487336209248	38.50509683481002
10	14.883868947192951	28.47458654516432	33.88814240829233	22.753402099350392
11	34.41651596738834	23.548993261469143	23.918753725387187	18.11573704575533
12	23.053453623349466	25.78473071133427	30.26681349322611	20.89500217209016
13	27.996221573401492	22.226543951991758	25.8418112201085	23.935423254498247
14	22.00074760312377	20.750027782548518	27.374397623834394	29.874826990493318
15	23.77175878686239	29.010537162947177	23.828334158390835	23.389369891799603
16	23.275208874251135	28.4558964670701	24.96135700069709	23.307537657981673
17	22.5377084953982	27.83861875271511	26.186314821737067	23.43735793014962
18	23.27773456048008	27.379448996292293	27.250133861370134	22.09268258185749
19	24.505723204994798	26.72125516502834	26.650030813371995	22.12299081660487
20	24.12181889819464	26.554054736671954	26.8894658678763	22.434660497257106
21	23.176707111322145	27.201640685774326	27.004132022670557	22.61752018023297
22	23.065071780002626	27.032924845680572	26.802582261600477	23.099421112716325
23	22.85291413677096	26.8945172403342	27.45067334794864	22.8018952749462
24	23.280765383954822	27.319842801289113	26.00699109948173	23.392400715274338
25	23.347948637644848	26.888455593384723	26.8894658678763	22.87412990109413
26	23.34693836315327	27.343584251841225	26.66771061697463	22.641766768030873
27	23.315619853914306	26.865724417324188	26.54799308972248	23.270662639039028
28	22.95242617419153	26.762171281937302	27.244072214420655	23.041330329450513
29	24.02836850772355	26.910681632199466	26.46060434620086	22.600345513876118
30	23.414121616843296	26.75004798803835	26.95109261186264	22.88473778325571
31	23.304001697261146	26.900073750037883	26.782881909014677	23.01304264368629
32	22.766535667740925	26.83188022185628	26.97483406241476	23.426750047988037
33	22.74228907994302	26.868250103553137	27.38854146671651	23.00091934978734
34	23.31056848145641	26.53890061929826	27.160724568865362	22.989806330379963
35	23.127708798480548	27.123344412676925	26.842993241263652	22.90595354757888
36	23.876322196740855	26.774294575836254	26.43686289564875	22.912520331774143
37	23.254498247173757	26.493438267177194	26.8111695947789	23.44089389087015
38	23.31511471666852	26.90310457351262	26.593960579089337	23.18782013072952
39	23.03122758453472	26.838447006051545	27.077376923310066	23.052948486103674
40	23.095885151995798	27.38500550599598	27.10061323661639	22.418496105391835
41	22.992332016608913	26.315124819413434	27.125364961660082	23.56717820231757
42	22.8902942929594	26.87885798571472	27.086469393734276	23.144378327591607
43	23.262075305860602	25.901922552357476	27.277916409888565	23.558085731893357
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	5.0
2	6.0
3	22.5
4	39.0
5	39.0
6	52.5
7	66.0
8	74.0
9	82.0
10	99.5
11	117.0
12	117.0
13	182.5
14	248.0
15	484.5
16	721.0
17	803.5
18	886.0
19	886.0
20	849.5
21	813.0
22	1000.0
23	1187.0
24	1618.5
25	2050.0
26	2050.0
27	2819.0
28	3588.0
29	4516.0
30	5444.0
31	6580.0
32	7716.0
33	7716.0
34	9081.5
35	10447.0
36	11513.0
37	12579.0
38	13694.0
39	14809.0
40	14809.0
41	15419.0
42	16029.0
43	16411.0
44	16793.0
45	16451.5
46	16110.0
47	16110.0
48	15612.5
49	15115.0
50	14928.0
51	14741.0
52	14003.5
53	13266.0
54	13266.0
55	12583.0
56	11900.0
57	10499.5
58	9099.0
59	8231.5
60	7364.0
61	7364.0
62	6526.0
63	5688.0
64	4815.5
65	3943.0
66	3337.0
67	2731.0
68	2731.0
69	2217.5
70	1704.0
71	1405.5
72	1107.0
73	878.5
74	650.0
75	650.0
76	518.0
77	386.0
78	299.0
79	212.0
80	173.0
81	134.0
82	134.0
83	106.5
84	79.0
85	77.0
86	75.0
87	51.5
88	28.0
89	28.0
90	19.0
91	10.0
92	5.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	197966.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.50043337328864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.51693404888191	58.3993054242776
2	7.684881574629193	10.374656734197426
3	2.190882291704909	4.436565124798354
4	0.9579963967050282	2.586606877905534
5	0.6127743018232265	2.068126546654108
6	0.3756205714074602	1.5212730812355524
7	0.28307679355419135	1.3375464369980212
8	0.21468140728166285	1.159287042295978
9	0.14936167405326597	0.9073779955159801
>10	0.9326881140679435	12.020519777648255
>50	0.05835447228758297	2.753928423018784
>100	0.02274835360363404	2.4348065354544257
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	446	0.22529121162219776	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	344	0.17376721255165029	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	215	0.10860450784478143	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.05137245789681E-4	0.0
5	0.0	0.0	0.0	5.05137245789681E-4	0.0
6	0.0	0.0	5.05137245789681E-4	5.05137245789681E-4	0.0
7	0.0	0.0	0.0015154117373690431	5.05137245789681E-4	0.0
8	0.0	0.0	0.0035359607205277675	5.05137245789681E-4	0.0
9	0.0	0.0	0.0035359607205277675	0.002020548983158724	0.0
10	0.0	0.0	0.004041097966317448	0.002020548983158724	0.0
11	0.0	0.0	0.007071921441055535	0.002020548983158724	0.0
12	0.0	0.0	0.0075770586868452155	0.002020548983158724	0.0
13	0.0	0.0	0.0075770586868452155	0.002020548983158724	0.0
14	0.0	0.0	0.0075770586868452155	0.002020548983158724	0.0
15	0.0	0.0	0.00959760767000394	0.002020548983158724	0.0
16	0.0	0.0	0.010607882161583301	0.002020548983158724	0.0
17	0.0	0.0	0.011618156653162664	0.002020548983158724	0.0
18	0.0	0.0	0.011618156653162664	0.002020548983158724	0.0
19	0.0	0.0	0.011618156653162664	0.002020548983158724	0.0
20	0.0	0.0	0.011618156653162664	0.002020548983158724	0.0
21	0.0	0.0	0.012123293898952345	0.002020548983158724	0.0
22	0.0	0.0	0.012123293898952345	0.002020548983158724	0.0
23	0.0	0.0	0.012628431144742026	0.002020548983158724	0.0
24	0.0	0.0	0.012628431144742026	0.0025256862289484054	0.0
25	0.0	0.0	0.012628431144742026	0.0025256862289484054	0.0
26	0.0	0.0	0.012628431144742026	0.0030308234747380863	0.0
27	0.0	0.0	0.012628431144742026	0.0035359607205277675	0.0
28	0.0	0.0	0.012628431144742026	0.00909247042421426	0.0
29	0.0	0.0	0.012628431144742026	0.01818494084842852	0.0
30	0.0	0.0	0.012628431144742026	0.03131850923896023	0.0
31	0.0	0.0	0.012628431144742026	0.08991442975056323	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	105	0.0	29.95238	1
CCCGAAA	25	0.0054854937	29.6	37
TACATCG	40	0.0019255758	23.125	5
GAATTAT	40	0.0019255758	23.125	1
GTATCAA	305	0.0	22.442621	1
CTACAGT	50	2.6911678E-4	22.2	4
TCTGTAC	45	0.0038147983	20.555553	3
TACTGAG	45	0.0038147983	20.555553	2
TAATACA	50	0.0070148287	18.5	4
TGACAAT	80	1.6056827E-5	18.5	33
AATACAG	60	9.2000474E-4	18.5	5
AAAGCGT	50	0.0070148287	18.5	35
TCGAAAC	50	0.0070148287	18.5	16
CTGCGTC	75	2.0563958E-4	17.266666	9
ACAATTA	75	2.0563958E-4	17.266666	35
CGTCAGA	75	2.0563958E-4	17.266666	12
GTTCAGC	65	0.0015736959	17.076923	21
GTCTGGA	70	0.0025823424	15.857143	1
GGGTTCA	95	7.0126014E-5	15.578948	19
ACTGACA	85	5.336346E-4	15.235294	31
>>END_MODULE