FastQCFastQC Report
Fri 10 Feb 2017
ERR1632914.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632914.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222259
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19210.8643069571985837No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15530.6987343594635088No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8210.36938886614265337No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.19616753427307781No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC4080.18356961922801776No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC3950.1777205872428113No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3930.17682073616816416No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG3660.16467274666042772No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3470.15612416145127983No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT3370.1516249060780441No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3200.14397617194354334No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC3150.14172654425692546No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG3110.13992684210763118No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA3050.13722728888368974No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG3030.1363274378090426No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT2960.1331779590477776No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2850.12822877813721828No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2810.126429075987924No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC2800.1259791504506004No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2750.12372952276398257No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA2720.12237974615201183No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG2670.12013011846539398No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG2640.11878034185342325No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT2570.11563086309215825No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA2550.11473101201751111No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2480.11158153325624609No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG2440.1097818311069518No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC2420.10888198003230465No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT2400.1079821289576575No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC2400.1079821289576575No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC2380.10708227788301036No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT2290.1030329480470982No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG2280.10258302250977464No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG2280.10258302250977464No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2270.10213309697245106No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT2240.10078332036048035No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTGC200.001838743337.09
GGTATCA3550.030.7464791
CGCCACA453.98916E-628.77777915
ACTAGTG358.8468066E-426.4285728
TCGCCAC509.048079E-625.90000214
CTCGCCA509.048079E-625.90000213
TAGCTGA509.048079E-625.9000026
TGACTCG509.048079E-625.90000210
CTGACTC601.3293138E-624.6666669
GTATCAA9800.023.974491
CCGGCAG701.9084473E-723.78571516
GCAGCTT701.9084473E-723.78571519
GCTGACT551.8937219E-523.5454548
AACATGT551.8937219E-523.54545422
AGCTGAC551.8937219E-523.5454547
TGCTCGC802.7008355E-823.12500210
GGCAGCT753.7127393E-722.218
CGGCAGC753.7127393E-722.217
GCCGGCA753.7127393E-722.215
CGCCGGC753.7127393E-722.214
Produced by FastQC (version 0.11.5)