##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632914.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	222259
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.434997007995175	33.0	33.0	33.0	14.0	33.0
2	30.324603278157465	33.0	33.0	33.0	14.0	33.0
3	30.771424329273504	33.0	33.0	33.0	27.0	33.0
4	30.957666506193224	33.0	33.0	33.0	27.0	33.0
5	31.098623677781326	33.0	33.0	33.0	27.0	33.0
6	33.23754268668535	37.0	33.0	37.0	27.0	37.0
7	33.5220216054243	37.0	33.0	37.0	27.0	37.0
8	33.67240921627471	37.0	33.0	37.0	27.0	37.0
9	33.65026388132764	37.0	33.0	37.0	27.0	37.0
10	33.66403160276974	37.0	33.0	37.0	27.0	37.0
11	33.76706455081684	37.0	33.0	37.0	27.0	37.0
12	33.73901169356471	37.0	33.0	37.0	27.0	37.0
13	33.78589393455383	37.0	33.0	37.0	27.0	37.0
14	33.809528523029435	37.0	33.0	37.0	27.0	37.0
15	33.84963488542646	37.0	33.0	37.0	27.0	37.0
16	33.858525414043974	37.0	33.0	37.0	27.0	37.0
17	33.821649517004936	37.0	33.0	37.0	27.0	37.0
18	33.84322794577498	37.0	33.0	37.0	27.0	37.0
19	33.81750570280619	37.0	33.0	37.0	27.0	37.0
20	33.77824070116396	37.0	33.0	37.0	27.0	37.0
21	33.75062877093841	37.0	33.0	37.0	27.0	37.0
22	33.727930027580435	37.0	33.0	37.0	27.0	37.0
23	33.758286503583655	37.0	33.0	37.0	27.0	37.0
24	33.756531793988096	37.0	33.0	37.0	27.0	37.0
25	33.74344346010735	37.0	33.0	37.0	27.0	37.0
26	33.62904989224283	37.0	33.0	37.0	27.0	37.0
27	33.569655222060746	37.0	33.0	37.0	27.0	37.0
28	33.54224125907162	37.0	33.0	37.0	27.0	37.0
29	33.55494715624564	37.0	33.0	37.0	27.0	37.0
30	33.488767608960714	37.0	33.0	37.0	27.0	37.0
31	33.44859825698847	37.0	33.0	37.0	27.0	37.0
32	33.47605721253132	37.0	33.0	37.0	27.0	37.0
33	33.43501950427204	37.0	33.0	37.0	27.0	37.0
34	33.34786442843709	37.0	33.0	37.0	22.0	37.0
35	33.35275061977243	37.0	33.0	37.0	22.0	37.0
36	33.27799999100149	37.0	33.0	37.0	22.0	37.0
37	33.29179020872046	37.0	33.0	37.0	22.0	37.0
38	33.12504780458834	37.0	33.0	37.0	14.0	37.0
39	33.212040907229856	37.0	33.0	37.0	14.0	37.0
40	33.186152191812255	37.0	33.0	37.0	14.0	37.0
41	33.190867411443406	37.0	33.0	37.0	14.0	37.0
42	33.03147229133578	37.0	33.0	37.0	14.0	37.0
43	32.66817991622386	37.0	33.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	6.0
16	15.0
17	28.0
18	24.0
19	38.0
20	118.0
21	300.0
22	703.0
23	1316.0
24	2250.0
25	3453.0
26	4909.0
27	6514.0
28	8189.0
29	10347.0
30	12356.0
31	14715.0
32	17633.0
33	21330.0
34	28200.0
35	41365.0
36	48448.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.12292865530755	21.419605055363338	13.342541809330555	23.11492447999856
2	17.565992828186936	25.09729639744622	35.61385590684742	21.722854867519427
3	18.747497289198638	28.94056033726418	29.86110798662821	22.450834386908966
4	13.052339837756852	20.04508253883982	39.05173693753684	27.85084068586649
5	14.7863528586019	37.51119189774093	34.21998659221899	13.482468651438188
6	27.169203496821275	42.073886771739275	16.609901061374345	14.147008670065103
7	26.34493991244449	32.89675558695036	22.86836528554524	17.889939215059908
8	24.733756563288775	34.542133276942664	20.034284325944057	20.6898258338245
9	27.114312581267804	14.318880225322708	20.07027836892994	38.49652882447955
10	16.652194061882756	27.377968946139415	32.80722040502297	23.162616586954858
11	34.06386243076771	24.428707048983394	23.12842224611827	18.37900827413063
12	23.531555527560187	27.651973598369473	28.31336413823512	20.503106735835217
13	29.268556053973064	22.88186305166495	25.265568548405238	22.584012345956744
14	22.669948123585545	21.55368286548576	28.4712880018357	27.305081009092998
15	24.9785160555928	28.02721149649733	25.894564449583594	21.099707998326277
16	22.047251179929724	28.174337147202138	25.86666906626953	23.911742606598608
17	22.250167597262653	27.01757859074323	25.972851493077897	24.75940231891622
18	23.481163867379948	25.91211154553921	28.340809596011862	22.26591499106898
19	26.27025227324878	24.95467000211465	28.474437480596958	20.30064024403961
20	25.223275547896822	25.27951624006227	28.68455270652707	20.81265550551384
21	22.764432486423498	27.19664895459801	28.452391129268108	21.586527429710383
22	22.586261973643364	26.737274980990644	28.40154954355054	22.274913501815448
23	22.78287943345376	26.428176136849352	29.49216904602288	21.296775383674003
24	22.35185076869778	27.446357627812596	27.237592178494456	22.96419942499516
25	23.18331316167174	27.091366378864297	28.982403412235275	20.742917047228683
26	22.634404006136986	27.258738678748667	28.590968194763768	21.515889120350582
27	22.862066328022713	26.837158450276476	27.966021623421323	22.334753598279487
28	22.068847605721253	26.56900283003163	29.493518822634858	21.868630741612265
29	23.76956613680436	26.71432877858714	27.633526651339203	21.882578433269295
30	23.304343131211784	26.091181909393995	28.892418304770562	21.71205665462366
31	23.080280213624647	26.392632019400793	28.500983087299055	22.026104679675516
32	21.315222330704266	26.46596988198453	28.935611156353623	23.283196630957576
33	21.356165554600715	26.693182278332937	29.456175003037	22.49447716402935
34	22.17053077715638	26.15462141015662	29.362590491273693	22.312257321413306
35	22.158832713185966	27.23624240188249	28.422246118267424	22.182678766664118
36	23.31154193980896	27.018028516280555	27.674919800772972	21.99550974313751
37	22.85396766835089	26.388582689564878	27.973670357555825	22.78377928452841
38	22.338353002578074	26.924893930054576	27.977269761854412	22.759483305512937
39	21.82768751771582	26.641890767078046	28.736744068856606	22.79367764634953
40	21.973013466271333	27.516546011635075	29.14752608443303	21.362914437660567
41	22.54711845189621	26.1771176870228	28.337660117250596	22.938103743830396
42	21.627920579144153	27.70371503516168	28.177486625963404	22.490877759730765
43	22.6443023679581	25.908062215703303	28.351157883370306	23.096477532968294
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	37.0
1	30.5
2	24.0
3	88.5
4	153.0
5	153.0
6	229.5
7	306.0
8	326.0
9	346.0
10	457.0
11	568.0
12	568.0
13	921.0
14	1274.0
15	2093.5
16	2913.0
17	2969.5
18	3026.0
19	3026.0
20	2391.0
21	1756.0
22	1355.5
23	955.0
24	1083.5
25	1212.0
26	1212.0
27	1593.0
28	1974.0
29	2757.5
30	3541.0
31	4746.5
32	5952.0
33	5952.0
34	8062.0
35	10172.0
36	11442.5
37	12713.0
38	14314.0
39	15915.0
40	15915.0
41	17900.0
42	19885.0
43	20633.5
44	21382.0
45	20970.0
46	20558.0
47	20558.0
48	20334.0
49	20110.0
50	19702.5
51	19295.0
52	17987.5
53	16680.0
54	16680.0
55	15614.0
56	14548.0
57	11910.5
58	9273.0
59	8264.0
60	7255.0
61	7255.0
62	6097.0
63	4939.0
64	3559.0
65	2179.0
66	1843.0
67	1507.0
68	1507.0
69	1192.0
70	877.0
71	655.5
72	434.0
73	367.0
74	300.0
75	300.0
76	204.5
77	109.0
78	69.5
79	30.0
80	26.0
81	22.0
82	22.0
83	21.0
84	20.0
85	16.5
86	13.0
87	8.0
88	3.0
89	3.0
90	2.0
91	1.0
92	1.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	222259.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.573281621891574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.19626392048856	30.50810090929951
2	6.578852831996168	4.94378180411142
3	2.8224164770686144	3.1814234744149843
4	1.6704586277092566	2.510584498265537
5	1.2022512273979165	2.2586261973643365
6	0.8717518860016765	1.9652747470293668
7	0.6873428331936295	1.807800808966116
8	0.5795713088252904	1.7421116805168744
9	0.4430607112920608	1.498252039287498
>10	3.3540893306190873	24.959619183025207
>50	0.3927673332535026	10.157518930616984
>100	0.19758112800862174	12.534475544297418
>500	0.0011974613818704347	0.36938886614265337
>1k	0.0023949227637408694	1.5630413166620925
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1921	0.8643069571985837	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1553	0.6987343594635088	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	821	0.36938886614265337	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	436	0.19616753427307781	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	408	0.18356961922801776	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	395	0.1777205872428113	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	393	0.17682073616816416	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	366	0.16467274666042772	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	347	0.15612416145127983	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	337	0.1516249060780441	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	320	0.14397617194354334	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	315	0.14172654425692546	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	311	0.13992684210763118	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	305	0.13722728888368974	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	303	0.1363274378090426	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	296	0.1331779590477776	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	285	0.12822877813721828	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	281	0.126429075987924	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	280	0.1259791504506004	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	275	0.12372952276398257	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	272	0.12237974615201183	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	267	0.12013011846539398	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	264	0.11878034185342325	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	257	0.11563086309215825	No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA	255	0.11473101201751111	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	248	0.11158153325624609	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	244	0.1097818311069518	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	242	0.10888198003230465	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	240	0.1079821289576575	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	240	0.1079821289576575	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	238	0.10708227788301036	No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT	229	0.1030329480470982	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	228	0.10258302250977464	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	228	0.10258302250977464	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	227	0.10213309697245106	No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT	224	0.10078332036048035	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.002699553223941438	0.0	0.0
2	4.4992553732357295E-4	0.0	0.002699553223941438	0.0	0.0
3	4.4992553732357295E-4	0.0	0.004499255373235729	0.0	0.0
4	4.4992553732357295E-4	0.0	0.006298957522530021	0.0	0.0
5	4.4992553732357295E-4	0.0	0.008548585209147886	0.0	0.0
6	4.4992553732357295E-4	0.0	0.014397617194354334	0.0	0.0
7	8.998510746471459E-4	0.0	0.030145011000679386	0.0	0.0
8	8.998510746471459E-4	0.0	0.03194471314997368	0.0	0.0
9	8.998510746471459E-4	0.0	0.03869359620982727	0.0	0.0
10	0.0022496276866178647	0.0	0.042293000508415855	0.0	0.0
11	0.0031494787612650105	0.0	0.05939017092671163	0.0	0.0
12	0.0031494787612650105	0.0	0.06253964968797664	0.0	0.0
13	0.0031494787612650105	0.0	0.07108823489712453	0.0	0.0
14	0.0031494787612650105	0.0	0.07603741580768383	0.0	0.0
15	0.0031494787612650105	0.0	0.08053667118091956	0.0	0.0
16	0.0031494787612650105	0.0	0.08728555424077315	4.4992553732357295E-4	0.0
17	0.0031494787612650105	0.0	0.08908525639006744	4.4992553732357295E-4	0.0
18	0.0035994042985885836	0.0	0.09178480961400888	4.4992553732357295E-4	0.0
19	0.0035994042985885836	0.0	0.0931345862259796	4.4992553732357295E-4	0.0
20	0.0035994042985885836	0.0	0.09538421391259747	0.001349776611970719	0.0
21	0.0035994042985885836	0.0	0.09853369267386247	0.0022496276866178647	0.0
22	0.0035994042985885836	0.0	0.09988346928583319	0.0031494787612650105	0.0
23	0.0035994042985885836	0.0	0.10033339482315677	0.004049329835912156	0.0
24	0.0035994042985885836	0.0	0.10033339482315677	0.004049329835912156	0.0
25	0.0035994042985885836	0.0	0.10078332036048034	0.004049329835912156	0.0
26	0.0035994042985885836	0.0	0.10123324589780391	0.005849031985206449	0.0
27	0.0035994042985885836	0.0	0.10123324589780391	0.00764873413450074	0.0
28	0.0035994042985885836	0.0	0.10168317143512749	0.021596425791531502	0.0
29	0.0035994042985885836	0.0	0.10168317143512749	0.05894024538938806	0.0
30	0.0035994042985885836	0.0	0.10168317143512749	0.1079821289576575	0.0
31	0.0035994042985885836	0.0	0.10168317143512749	0.22721239634840434	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGTGC	20	0.0018387433	37.0	9
GGTATCA	355	0.0	30.746479	1
CGCCACA	45	3.98916E-6	28.777779	15
ACTAGTG	35	8.8468066E-4	26.428572	8
TCGCCAC	50	9.048079E-6	25.900002	14
CTCGCCA	50	9.048079E-6	25.900002	13
TAGCTGA	50	9.048079E-6	25.900002	6
TGACTCG	50	9.048079E-6	25.900002	10
CTGACTC	60	1.3293138E-6	24.666666	9
GTATCAA	980	0.0	23.97449	1
CCGGCAG	70	1.9084473E-7	23.785715	16
GCAGCTT	70	1.9084473E-7	23.785715	19
GCTGACT	55	1.8937219E-5	23.545454	8
AACATGT	55	1.8937219E-5	23.545454	22
AGCTGAC	55	1.8937219E-5	23.545454	7
TGCTCGC	80	2.7008355E-8	23.125002	10
GGCAGCT	75	3.7127393E-7	22.2	18
CGGCAGC	75	3.7127393E-7	22.2	17
GCCGGCA	75	3.7127393E-7	22.2	15
CGCCGGC	75	3.7127393E-7	22.2	14
>>END_MODULE