FastQCFastQC Report
Fri 10 Feb 2017
ERR1632913.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632913.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483969
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32810.6779359835030756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25320.5231740049465978No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11920.24629676694168426No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6850.14153799106967596No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG6750.13947174302486315No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4900.1012461541958266No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAA250.005493237629.610
TCGGGTA250.005493237629.625
GTCGTAT250.005493237629.613
GTTCCGA250.005493237629.633
GGTATCA6250.025.7521
GTATCAA17200.023.7703481
TAGACTA502.6989705E-422.25
TTCGCCG2100.022.0238124
TGCTCGC2200.021.86363610
CCGGCAG2250.021.37777716
GCCGGCA2250.021.37777715
CTGCTCG2600.021.3461539
ACCGACC705.091855E-621.1428598
CCGCTCT2100.021.14285728
CGCTCTC2150.020.65116329
TTGCTAT450.003822655620.55555517
CGAGTCG1450.020.41379421
GAGTCGC1450.020.41379422
GCCGTCC1550.020.29032127
TGTCCGG1750.020.08571414
Produced by FastQC (version 0.11.5)