##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632913.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	483969
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.720292002173693	33.0	33.0	33.0	27.0	33.0
2	31.254003872148836	33.0	33.0	33.0	27.0	33.0
3	31.68855856470146	33.0	33.0	33.0	27.0	33.0
4	31.813932297316562	33.0	33.0	33.0	27.0	33.0
5	31.928592533819316	33.0	33.0	33.0	33.0	33.0
6	34.81202101787511	37.0	37.0	37.0	27.0	37.0
7	34.935892588161636	37.0	37.0	37.0	27.0	37.0
8	35.13066539385787	37.0	37.0	37.0	33.0	37.0
9	35.1693145635361	37.0	37.0	37.0	33.0	37.0
10	35.24258371920516	37.0	37.0	37.0	33.0	37.0
11	35.28608030679651	37.0	37.0	37.0	33.0	37.0
12	35.19901687918028	37.0	37.0	37.0	33.0	37.0
13	35.24751791953617	37.0	37.0	37.0	33.0	37.0
14	35.19555591370521	37.0	37.0	37.0	33.0	37.0
15	35.264611989610906	37.0	37.0	37.0	33.0	37.0
16	35.26371937045555	37.0	37.0	37.0	33.0	37.0
17	35.23719701055233	37.0	37.0	37.0	33.0	37.0
18	35.20791827575733	37.0	37.0	37.0	33.0	37.0
19	35.238994646351316	37.0	37.0	37.0	33.0	37.0
20	35.163111686905566	37.0	37.0	37.0	33.0	37.0
21	35.216848599807015	37.0	37.0	37.0	33.0	37.0
22	35.239703369430686	37.0	37.0	37.0	33.0	37.0
23	35.20865179381324	37.0	37.0	37.0	33.0	37.0
24	35.200328946688735	37.0	37.0	37.0	33.0	37.0
25	35.22213406230564	37.0	37.0	37.0	33.0	37.0
26	35.17878417832547	37.0	37.0	37.0	33.0	37.0
27	35.10635598561065	37.0	37.0	37.0	33.0	37.0
28	35.101324671621526	37.0	37.0	37.0	33.0	37.0
29	35.134359845362	37.0	37.0	37.0	33.0	37.0
30	35.09275594097969	37.0	37.0	37.0	33.0	37.0
31	35.102279278218234	37.0	37.0	37.0	33.0	37.0
32	35.113209730375296	37.0	37.0	37.0	33.0	37.0
33	35.06780392959053	37.0	37.0	37.0	27.0	37.0
34	35.0177656006893	37.0	37.0	37.0	27.0	37.0
35	34.99515671458296	37.0	37.0	37.0	27.0	37.0
36	34.97790354340877	37.0	37.0	37.0	27.0	37.0
37	34.9845444646248	37.0	37.0	37.0	27.0	37.0
38	34.82435858495069	37.0	37.0	37.0	27.0	37.0
39	34.87520068434135	37.0	37.0	37.0	27.0	37.0
40	34.92590227886497	37.0	37.0	37.0	27.0	37.0
41	34.9073267089421	37.0	37.0	37.0	27.0	37.0
42	34.767852899669194	37.0	37.0	37.0	27.0	37.0
43	34.58078513293207	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	10.0
16	32.0
17	25.0
18	16.0
19	15.0
20	38.0
21	114.0
22	286.0
23	659.0
24	1395.0
25	2502.0
26	4072.0
27	6150.0
28	8542.0
29	11733.0
30	15155.0
31	19390.0
32	24544.0
33	31864.0
34	45577.0
35	82671.0
36	229174.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.81135568600468	20.02029055580006	14.008748494221738	24.159605263973518
2	15.628686961354962	23.98893317547198	38.47622471687236	21.906155146300694
3	17.933999904952593	27.374687221702214	31.208610468852342	23.48270240449285
4	12.572086228663407	18.198479654688626	39.5198866043073	29.709547512340666
5	13.821546421361699	36.92901818091654	34.758424609840716	14.491010787881041
6	28.172878841413397	40.46746795765844	17.487483702468545	13.872169498459613
7	25.555355818244557	33.199440460029464	23.28206971934153	17.96313400238445
8	24.216013835596907	35.31734470596257	20.941423934177603	19.525217524262917
9	26.65439315328048	15.12700193607442	20.076285877814488	38.14231903283061
10	14.860249313489089	28.35574179337933	33.54243763546839	23.241571257663196
11	33.4374722347919	24.29845713258494	23.88954664451649	18.374523988106674
12	23.10499226190107	26.0626197132461	30.113912254710527	20.7184757701423
13	28.554514855290318	22.581198382541032	26.434131111703437	22.430155650465217
14	22.525616310135565	21.48691341800818	28.450789203440717	27.536681068415543
15	24.045961621508816	28.498932782884857	25.381790982480286	22.073314613126048
16	22.075794110779825	28.931811748273134	25.580564044391274	23.41183009655577
17	22.046660013347964	27.247199717337267	26.463471833939778	24.24266843537499
18	23.406251226834776	26.27151739057667	28.12390876275133	22.19832261983722
19	25.245211986718157	25.742351266300112	28.36751940723476	20.644917339746968
20	25.089416884139272	25.440059177344004	27.927615198494117	21.542908740022604
21	22.859935243786275	27.085619120232906	27.585857771882083	22.468587864098733
22	22.669633798859017	26.75749893071664	27.847444774355385	22.725422496068965
23	22.53594755035963	26.546535005341248	28.54728298713347	22.370234457165644
24	22.829561397527527	26.687659746801966	27.973899154697925	22.508879700972585
25	22.904772826358712	26.50438354522707	28.586748324789397	22.00409530362482
26	22.817783783672095	26.72092634032345	27.921416454359683	22.539873421644774
27	22.950023658540115	26.65914552378355	27.901373848324994	22.489456969351345
28	22.24068070475588	26.721959464345858	28.163374100407257	22.873985730491004
29	22.942171915969826	26.902136293853534	27.666648070434263	22.48904371974238
30	22.82852827350512	27.004828821680725	28.117710018616894	22.048932886197257
31	22.979984255189898	26.665137643113507	27.905712969219103	22.449165132477493
32	21.827431095793326	26.615754314842476	28.245404147786324	23.31141044157787
33	22.14501342028105	26.48847343528201	28.784901512286943	22.581611632149993
34	22.244399951236545	26.326272963764207	28.329707068014688	23.09962001698456
35	22.32581012420217	26.993257832629773	28.212550803873803	22.468381239294253
36	22.50226770722918	26.795931144350156	28.24726377102666	22.454537377394008
37	22.91675706501863	26.43619735974825	28.009645245873187	22.637400329359938
38	22.882250722670253	26.671543012052425	27.775745967200375	22.670460298076943
39	22.487184096502048	26.39797177091921	28.118743142639303	22.99610098993944
40	22.213819480173317	26.87878769094715	28.437771840758398	22.46962098812114
41	22.524996435722123	26.18308197425868	28.443350710479393	22.848570879539807
42	22.327669747442503	26.628151803111354	28.11998289146619	22.924195557979953
43	22.270434676601187	26.17440373247047	28.33342631449535	23.221735276432995
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	27.0
1	43.0
2	59.0
3	192.0
4	325.0
5	325.0
6	413.5
7	502.0
8	490.0
9	478.0
10	670.5
11	863.0
12	863.0
13	1362.5
14	1862.0
15	3155.5
16	4449.0
17	4903.0
18	5357.0
19	5357.0
20	4630.0
21	3903.0
22	4101.5
23	4300.0
24	5467.5
25	6635.0
26	6635.0
27	8499.0
28	10363.0
29	13082.5
30	15802.0
31	17833.0
32	19864.0
33	19864.0
34	22226.5
35	24589.0
36	26523.0
37	28457.0
38	30061.0
39	31665.0
40	31665.0
41	33446.0
42	35227.0
43	34874.5
44	34522.0
45	35548.0
46	36574.0
47	36574.0
48	36331.0
49	36088.0
50	37017.5
51	37947.0
52	37398.0
53	36849.0
54	36849.0
55	33022.0
56	29195.0
57	26286.0
58	23377.0
59	20563.5
60	17750.0
61	17750.0
62	15261.5
63	12773.0
64	10219.0
65	7665.0
66	6860.5
67	6056.0
68	6056.0
69	4970.5
70	3885.0
71	3328.5
72	2772.0
73	2282.0
74	1792.0
75	1792.0
76	1297.5
77	803.0
78	614.0
79	425.0
80	342.0
81	259.0
82	259.0
83	212.5
84	166.0
85	171.0
86	176.0
87	140.5
88	105.0
89	105.0
90	76.0
91	47.0
92	29.0
93	11.0
94	8.0
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	483969.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.02613108786589
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.9432031000528	57.58715312146527
2	5.463425177671342	6.996039532286646
3	1.4656513915190232	2.8151996436753013
4	0.7054070112259001	1.8065792708419668
5	0.4182445013191741	1.338928863412027
6	0.30439862776504517	1.1693679866550768
7	0.21759595665776077	0.9752279071611563
8	0.16248133146111854	0.832244082196845
9	0.13341087606172342	0.7687604015347441
>10	1.0193316529784018	13.2336727218244
>50	0.10939411648812315	4.769218708873352
>100	0.05583319869792068	5.969057432096594
>500	6.492232406734962E-4	0.2826573157260226
>1k	9.738348610102444E-4	1.4558930122505798
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3281	0.6779359835030756	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2532	0.5231740049465978	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1192	0.24629676694168426	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	685	0.14153799106967596	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	675	0.13947174302486315	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	490	0.1012461541958266	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	6.198744134438363E-4	0.0	0.0
2	4.1324960896255754E-4	0.0	0.0010331240224063939	0.0	0.0
3	4.1324960896255754E-4	0.0	0.0018596232403315087	0.0	0.0
4	4.1324960896255754E-4	0.0	0.0022728728492940665	0.0	0.0
5	4.1324960896255754E-4	0.0	0.002892747262737903	0.0	0.0
6	4.1324960896255754E-4	0.0	0.005165620112031969	0.0	0.0
7	4.1324960896255754E-4	0.0	0.011570989050951611	0.0	0.0
8	4.1324960896255754E-4	0.0	0.013637237095764398	0.0	0.0
9	6.198744134438363E-4	0.0	0.018802857207796366	6.198744134438363E-4	0.0
10	0.0016529984358502302	0.0	0.022108854079496826	6.198744134438363E-4	0.0
11	0.0016529984358502302	0.0	0.033886467934929713	0.0010331240224063939	0.0
12	0.0016529984358502302	0.0	0.037399089611111457	0.0012397488268876726	0.0
13	0.0016529984358502302	0.0	0.04029183687384936	0.0014463736313689514	0.0
14	0.0016529984358502302	0.0	0.043391208941068536	0.0014463736313689514	0.0
15	0.0016529984358502302	0.0	0.04690383061725028	0.0016529984358502302	0.0
16	0.0016529984358502302	0.0	0.0506230770979133	0.0016529984358502302	0.0
17	0.0016529984358502302	0.0	0.05144957631583841	0.0016529984358502302	0.0
18	0.0018596232403315087	0.0	0.05268932514272608	0.0020662480448127877	0.0
19	0.0018596232403315087	0.0	0.0535158243606512	0.0020662480448127877	0.0
20	0.0018596232403315087	0.0	0.054135698774095034	0.0020662480448127877	0.0
21	0.0018596232403315087	0.0	0.055582072405463985	0.0020662480448127877	0.0
22	0.0018596232403315087	0.0	0.05723507084131422	0.0020662480448127877	0.0
23	0.0018596232403315087	0.0	0.05847481966820189	0.0022728728492940665	0.0
24	0.0018596232403315087	0.0	0.058888069277164445	0.002892747262737903	0.0
25	0.0018596232403315087	0.0	0.059301318886127004	0.0030993720672191816	0.0
26	0.0018596232403315087	0.0	0.05950794369060828	0.0035126216761817387	0.0
27	0.0018596232403315087	0.0	0.05950794369060828	0.006198744134438363	0.0
28	0.0018596232403315087	0.0	0.05950794369060828	0.016943233967464857	0.0
29	0.0018596232403315087	0.0	0.05950794369060828	0.043804458550031095	0.0
30	0.0018596232403315087	0.0	0.05950794369060828	0.07789755128944209	0.0
31	0.0018596232403315087	0.0	0.05950794369060828	0.1820364527480066	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGAA	25	0.0054932376	29.6	10
TCGGGTA	25	0.0054932376	29.6	25
GTCGTAT	25	0.0054932376	29.6	13
GTTCCGA	25	0.0054932376	29.6	33
GGTATCA	625	0.0	25.752	1
GTATCAA	1720	0.0	23.770348	1
TAGACTA	50	2.6989705E-4	22.2	5
TTCGCCG	210	0.0	22.02381	24
TGCTCGC	220	0.0	21.863636	10
CCGGCAG	225	0.0	21.377777	16
GCCGGCA	225	0.0	21.377777	15
CTGCTCG	260	0.0	21.346153	9
ACCGACC	70	5.091855E-6	21.142859	8
CCGCTCT	210	0.0	21.142857	28
CGCTCTC	215	0.0	20.651163	29
TTGCTAT	45	0.0038226556	20.555555	17
CGAGTCG	145	0.0	20.413794	21
GAGTCGC	145	0.0	20.413794	22
GCCGTCC	155	0.0	20.290321	27
TGTCCGG	175	0.0	20.085714	14
>>END_MODULE