FastQCFastQC Report
Fri 10 Feb 2017
ERR1632912.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632912.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176066
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3240.18402190087807982No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2840.16130314768325513No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC2110.11984142310270011No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC2100.1192734542728295No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA1830.10393829586632286No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACT303.5872182E-430.83333217
GTATCAA2900.026.7931021
TAACGGC400.001924732623.12500236
ACGGGAC802.69265E-823.12500216
AACGGCC400.001924732623.12500237
GGTATCA802.69265E-823.1250021
TTAACGG400.001924732623.12500235
CTTACAC502.6895278E-422.23
TTAGAGG603.703266E-521.5833324
ACGGGGG603.703266E-521.58333216
ATTAGAG705.0615436E-621.1428593
GTGTTAA450.003813143820.5555571
CACATGT450.003813143820.55555728
CAGATTA450.003813143820.55555715
TGTTCCA450.003813143820.5555575
GCGCTTT450.003813143820.55555726
AACAGTG450.003813143820.5555577
TCGCCAT555.1193556E-420.18181813
ATAAGCC555.1193556E-420.1818183
CGGGGGA656.859794E-519.92307717