##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632912.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	176066
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.366243340565468	33.0	33.0	33.0	14.0	33.0
2	30.31115036406802	33.0	33.0	33.0	14.0	33.0
3	30.791958697306693	33.0	33.0	33.0	27.0	33.0
4	30.957368259629913	33.0	33.0	33.0	27.0	33.0
5	31.070235025501802	33.0	33.0	33.0	27.0	33.0
6	33.09771335749094	37.0	33.0	37.0	14.0	37.0
7	33.40056569695455	37.0	33.0	37.0	27.0	37.0
8	33.49157702225302	37.0	33.0	37.0	27.0	37.0
9	33.4806833800961	37.0	33.0	37.0	27.0	37.0
10	33.48275078663683	37.0	33.0	37.0	27.0	37.0
11	33.64772301296105	37.0	33.0	37.0	27.0	37.0
12	33.68026762691264	37.0	33.0	37.0	27.0	37.0
13	33.682176002181	37.0	33.0	37.0	27.0	37.0
14	33.722853929776335	37.0	33.0	37.0	27.0	37.0
15	33.75972078652324	37.0	33.0	37.0	27.0	37.0
16	33.76172003680438	37.0	33.0	37.0	27.0	37.0
17	33.751025183737916	37.0	33.0	37.0	27.0	37.0
18	33.73114059500415	37.0	33.0	37.0	27.0	37.0
19	33.73497438460577	37.0	33.0	37.0	27.0	37.0
20	33.723825156475414	37.0	33.0	37.0	27.0	37.0
21	33.73375893130985	37.0	33.0	37.0	27.0	37.0
22	33.733480626583216	37.0	33.0	37.0	27.0	37.0
23	33.69337066781775	37.0	33.0	37.0	27.0	37.0
24	33.702918223847874	37.0	33.0	37.0	27.0	37.0
25	33.70306589574364	37.0	33.0	37.0	27.0	37.0
26	33.647490145740804	37.0	33.0	37.0	27.0	37.0
27	33.54663024093238	37.0	33.0	37.0	27.0	37.0
28	33.53503231742642	37.0	33.0	37.0	27.0	37.0
29	33.56390785273704	37.0	33.0	37.0	27.0	37.0
30	33.5096611497961	37.0	33.0	37.0	27.0	37.0
31	33.51769790873877	37.0	33.0	37.0	27.0	37.0
32	33.53297059057399	37.0	33.0	37.0	27.0	37.0
33	33.51672100235139	37.0	33.0	37.0	27.0	37.0
34	33.4003328297343	37.0	33.0	37.0	27.0	37.0
35	33.41988799654675	37.0	33.0	37.0	27.0	37.0
36	33.38999011734236	37.0	33.0	37.0	22.0	37.0
37	33.435700248770345	37.0	33.0	37.0	27.0	37.0
38	33.32227119375688	37.0	33.0	37.0	22.0	37.0
39	33.38486135880863	37.0	33.0	37.0	27.0	37.0
40	33.404365408426386	37.0	33.0	37.0	27.0	37.0
41	33.42856656026717	37.0	33.0	37.0	27.0	37.0
42	33.30944645757841	37.0	33.0	37.0	22.0	37.0
43	33.008354821487394	37.0	33.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	15.0
16	16.0
17	14.0
18	20.0
19	48.0
20	118.0
21	243.0
22	543.0
23	1029.0
24	1794.0
25	2706.0
26	3830.0
27	5092.0
28	6418.0
29	8064.0
30	9698.0
31	11528.0
32	14006.0
33	17461.0
34	22738.0
35	33246.0
36	37436.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.47289084775028	21.998000749718855	12.915043222427952	24.614065180102916
2	16.65341406063635	24.892369906739518	35.65878704576693	22.795428986857203
3	18.87871593606943	27.653266388740587	30.256267536037623	23.21175013915236
4	14.76378176365681	20.2787591017005	36.53459498142742	28.422864153215272
5	15.430009201095043	37.34906228346188	34.67904081424011	12.541887701202958
6	29.473606488475912	39.85721263617052	17.543989185873478	13.125191689480081
7	26.057841945634024	32.70989288107869	23.527540808560428	17.704724364726864
8	24.043824474912817	36.99748957777197	20.630899776220282	18.32778617109493
9	25.456362954801044	16.29275385366851	20.044755943793806	38.206127247736646
10	14.603046584803426	28.99480876489498	33.99861415605512	22.403530494246475
11	33.37782422500653	23.984187747776403	24.259652630263652	18.378335396953418
12	22.24506719071257	26.271398225665376	30.272738632103867	21.21079595151818
13	27.393704633489712	22.37853986573217	26.803585019254143	23.424170481523973
14	21.944043710881147	21.39311394590665	27.27045539740779	29.392386945804418
15	23.802437722217803	29.20382129428737	24.147762770779142	22.845978212715686
16	23.183919666488702	29.023207206388513	24.694716753944544	23.09815637317824
17	22.46032737723354	28.583599332068655	25.827246600706555	23.128826689991254
18	22.31322345029705	28.337100859904808	27.38291322572217	21.966762464075973
19	24.611225335953563	26.82743971010871	27.46129292424432	21.100042029693412
20	24.014290095759545	26.60025217816046	27.587382004475597	21.7980757216044
21	22.425681278611428	28.03494144241364	26.75360376222553	22.7857735167494
22	22.484182068088103	27.662353890018515	26.69283109742937	23.16063294446401
23	22.501221132984224	27.603285131711974	27.23921711176491	22.6562766235389
24	23.22538139106926	28.062203946247433	25.98968568604955	22.722728976633764
25	23.343518907682345	27.359626503697477	27.030772551202393	22.266082037417785
26	23.074301682323675	27.482307770949532	26.82857564776845	22.614814898958347
27	22.974907137096316	27.29658196358184	26.664432655935844	23.064078243386003
28	22.291072665932095	27.452205422966387	27.518089807231377	22.73863210387014
29	23.462792361955177	27.502754648824872	26.714413912964456	22.320039076255497
30	22.825531334840342	27.58511012915611	26.901275657991892	22.688082878011652
31	23.204934513193916	27.178444446968754	26.954096759169854	22.662524280667476
32	22.430792998080264	27.39995229061829	27.21706632739995	22.952188383901493
33	22.322878920404847	27.347131189440322	27.556711687662585	22.773278202492246
34	22.873808685379345	27.136982722388197	27.083593652380355	22.9056149398521
35	22.863585246441676	27.687344518532825	26.962048322788046	22.487021912237456
36	23.404291572478503	27.53058512148853	26.55197482762146	22.513148478411505
37	23.52356502675133	26.66216078061636	26.707598287006007	23.1066759056263
38	23.537196278668226	27.206274919632413	26.79960923744505	22.456919564254314
39	22.70228209875842	27.32895618688446	27.109152249724534	22.85960946463258
40	22.69319459748049	27.35394681539877	27.234673361125942	22.718185225994798
41	22.575057080867403	27.153453818454445	27.310213215498734	22.961275885179422
42	23.016368861676874	27.050083491417993	27.114831938023244	22.818715708881896
43	23.08054933945225	26.583781082094216	27.151749911964828	23.183919666488702
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	3.0
2	4.0
3	10.5
4	17.0
5	17.0
6	35.0
7	53.0
8	56.5
9	60.0
10	73.0
11	86.0
12	86.0
13	145.5
14	205.0
15	407.5
16	610.0
17	681.0
18	752.0
19	752.0
20	802.0
21	852.0
22	1036.0
23	1220.0
24	1662.5
25	2105.0
26	2105.0
27	2753.0
28	3401.0
29	4559.5
30	5718.0
31	6512.0
32	7306.0
33	7306.0
34	8360.5
35	9415.0
36	10348.0
37	11281.0
38	12427.5
39	13574.0
40	13574.0
41	14526.5
42	15479.0
43	14813.0
44	14147.0
45	14168.5
46	14190.0
47	14190.0
48	13810.0
49	13430.0
50	13615.0
51	13800.0
52	13556.5
53	13313.0
54	13313.0
55	11610.0
56	9907.0
57	8617.5
58	7328.0
59	6379.5
60	5431.0
61	5431.0
62	4830.5
63	4230.0
64	3489.5
65	2749.0
66	2339.0
67	1929.0
68	1929.0
69	1599.5
70	1270.0
71	1074.5
72	879.0
73	744.5
74	610.0
75	610.0
76	454.5
77	299.0
78	246.0
79	193.0
80	136.5
81	80.0
82	80.0
83	69.5
84	59.0
85	52.5
86	46.0
87	33.5
88	21.0
89	21.0
90	16.0
91	11.0
92	7.0
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	176066.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.85616971489557
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.40231181139545	56.450330262175854
2	5.844057927066393	7.463583096288648
3	1.7465037976606559	3.3457512873338535
4	0.9081294736829275	2.319586791783832
5	0.5859485494869507	1.8708215010117806
6	0.38425314971893326	1.4722160605161227
7	0.2844395764408504	1.271425530678797
8	0.22256043728536623	1.1369485644092574
9	0.18563321202827313	1.0668443302798742
>10	1.3024263349348002	16.19194084890468
>50	0.10179883948160738	4.408220355436178
>100	0.031936890817759175	3.002331371181124
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	324	0.18402190087807982	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	284	0.16130314768325513	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	211	0.11984142310270011	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	210	0.1192734542728295	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	183	0.10393829586632286	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.679688298706167E-4	0.0	0.0
2	0.0	0.0	5.679688298706167E-4	0.0	0.0
3	0.0	0.0	5.679688298706167E-4	0.0	0.0
4	0.0	0.0	5.679688298706167E-4	0.0	0.0
5	0.0	0.0	0.0011359376597412335	0.0	0.0
6	0.0	0.0	0.00170390648961185	0.0	0.0
7	0.0	0.0	0.0034078129792237	0.0	0.0
8	0.0	0.0	0.003975781809094317	0.0	0.0
9	0.0	0.0	0.003975781809094317	0.0	0.0
10	5.679688298706167E-4	0.0	0.003975781809094317	0.0	0.0
11	5.679688298706167E-4	0.0	0.00511171946883555	5.679688298706167E-4	0.0
12	5.679688298706167E-4	0.0	0.005679688298706167	5.679688298706167E-4	0.0
13	5.679688298706167E-4	0.0	0.006247657128576784	5.679688298706167E-4	0.0
14	5.679688298706167E-4	0.0	0.006247657128576784	5.679688298706167E-4	0.0
15	5.679688298706167E-4	0.0	0.0068156259584474	5.679688298706167E-4	0.0
16	5.679688298706167E-4	0.0	0.0068156259584474	5.679688298706167E-4	0.0
17	5.679688298706167E-4	0.0	0.0068156259584474	5.679688298706167E-4	0.0
18	5.679688298706167E-4	0.0	0.0068156259584474	5.679688298706167E-4	0.0
19	5.679688298706167E-4	0.0	0.0068156259584474	5.679688298706167E-4	0.0
20	5.679688298706167E-4	0.0	0.007383594788318017	5.679688298706167E-4	0.0
21	5.679688298706167E-4	0.0	0.007951563618188634	5.679688298706167E-4	0.0
22	5.679688298706167E-4	0.0	0.007951563618188634	5.679688298706167E-4	0.0
23	5.679688298706167E-4	0.0	0.007951563618188634	5.679688298706167E-4	0.0
24	5.679688298706167E-4	0.0	0.007951563618188634	5.679688298706167E-4	0.0
25	5.679688298706167E-4	0.0	0.007951563618188634	5.679688298706167E-4	0.0
26	5.679688298706167E-4	0.0	0.007951563618188634	0.00170390648961185	0.0
27	5.679688298706167E-4	0.0	0.007951563618188634	0.0028398441493530835	0.0
28	5.679688298706167E-4	0.0	0.007951563618188634	0.007951563618188634	0.0
29	5.679688298706167E-4	0.0	0.007951563618188634	0.013063283087024185	0.0
30	5.679688298706167E-4	0.0	0.007951563618188634	0.026694535003918985	0.0
31	5.679688298706167E-4	0.0	0.007951563618188634	0.06929219724421523	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGACT	30	3.5872182E-4	30.833332	17
GTATCAA	290	0.0	26.793102	1
TAACGGC	40	0.0019247326	23.125002	36
ACGGGAC	80	2.69265E-8	23.125002	16
AACGGCC	40	0.0019247326	23.125002	37
GGTATCA	80	2.69265E-8	23.125002	1
TTAACGG	40	0.0019247326	23.125002	35
CTTACAC	50	2.6895278E-4	22.2	3
TTAGAGG	60	3.703266E-5	21.583332	4
ACGGGGG	60	3.703266E-5	21.583332	16
ATTAGAG	70	5.0615436E-6	21.142859	3
GTGTTAA	45	0.0038131438	20.555557	1
CACATGT	45	0.0038131438	20.555557	28
CAGATTA	45	0.0038131438	20.555557	15
TGTTCCA	45	0.0038131438	20.555557	5
GCGCTTT	45	0.0038131438	20.555557	26
AACAGTG	45	0.0038131438	20.555557	7
TCGCCAT	55	5.1193556E-4	20.181818	13
ATAAGCC	55	5.1193556E-4	20.181818	3
CGGGGGA	65	6.859794E-5	19.923077	17
>>END_MODULE