FastQCFastQC Report
Fri 10 Feb 2017
ERR1632911.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632911.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348931
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9240.26480880174017213No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6830.19574070518239994No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG4150.11893468909325913No Hit
CTTTAAAGCCTTAGGCCGTATGACAAAATGAAGAGACTGAAAT4030.11549561374598416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3840.11005041111279881No Hit
ACCCCACACAGGGCACCGAGCCCAATGGAGAAGGTTGGGAGTG3580.10259908119370305No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGTG250.00549116329.617
GGCTGCG358.8569685E-426.428577
GGTATCA2150.024.9534871
CTAGACT400.001928507523.1254
TTCGCCG400.001928507523.12524
GTATCAA5300.022.6886791
CTGCGCA502.6968788E-422.29
AGCCTTA957.1013346E-921.4210537
CTAGTTT1151.3460522E-1020.91304429
GCCGGCA450.003820548520.55555715
GCCGCTC450.003820548520.55555727
TGTACAC450.003820548520.5555573
CGGAGTC450.003820548520.55555727
GTTCTAG1101.7425918E-920.1818181
ATAAAGG555.1332335E-420.1818183
CTCTAGT1101.7425918E-920.18181827
CCTTGCA555.1332335E-420.1818189
TCTAGTT1202.3646862E-1020.04166828
ATAAGCA656.884536E-519.9230775
TAAAGCC1254.0927262E-1019.244
Produced by FastQC (version 0.11.5)