##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632911.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	348931
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40465020304874	33.0	33.0	33.0	27.0	33.0
2	31.03427898352396	33.0	33.0	33.0	27.0	33.0
3	31.463945593828004	33.0	33.0	33.0	27.0	33.0
4	31.61964686427976	33.0	33.0	33.0	27.0	33.0
5	31.718483023864316	33.0	33.0	33.0	27.0	33.0
6	34.52815886235388	37.0	37.0	37.0	27.0	37.0
7	34.680498436653664	37.0	37.0	37.0	27.0	37.0
8	34.85251238783599	37.0	37.0	37.0	27.0	37.0
9	34.928868458233865	37.0	37.0	37.0	27.0	37.0
10	34.99629439631331	37.0	37.0	37.0	27.0	37.0
11	35.05401354422508	37.0	37.0	37.0	33.0	37.0
12	34.94109723698955	37.0	37.0	37.0	27.0	37.0
13	34.99754392702282	37.0	37.0	37.0	27.0	37.0
14	34.95017066411411	37.0	37.0	37.0	27.0	37.0
15	35.00465421530331	37.0	37.0	37.0	27.0	37.0
16	34.99614536971493	37.0	37.0	37.0	27.0	37.0
17	34.96957851265723	37.0	37.0	37.0	27.0	37.0
18	34.93967002072043	37.0	37.0	37.0	27.0	37.0
19	34.96415050540078	37.0	37.0	37.0	27.0	37.0
20	34.89749835927447	37.0	37.0	37.0	27.0	37.0
21	34.95786846109976	37.0	37.0	37.0	27.0	37.0
22	34.96935210686353	37.0	37.0	37.0	27.0	37.0
23	34.94317501167853	37.0	37.0	37.0	27.0	37.0
24	34.93246229197177	37.0	37.0	37.0	27.0	37.0
25	34.927727831577016	37.0	37.0	37.0	27.0	37.0
26	34.902232819669216	37.0	37.0	37.0	27.0	37.0
27	34.82798604881768	37.0	37.0	37.0	27.0	37.0
28	34.81471694976944	37.0	37.0	37.0	27.0	37.0
29	34.84004000790987	37.0	37.0	37.0	27.0	37.0
30	34.826510112314466	37.0	37.0	37.0	27.0	37.0
31	34.818164622805085	37.0	37.0	37.0	27.0	37.0
32	34.846688886914606	37.0	37.0	37.0	27.0	37.0
33	34.825163141136784	37.0	37.0	37.0	27.0	37.0
34	34.76203031544919	37.0	37.0	37.0	27.0	37.0
35	34.73086369511451	37.0	37.0	37.0	27.0	37.0
36	34.734675336957736	37.0	37.0	37.0	27.0	37.0
37	34.75694908162359	37.0	37.0	37.0	27.0	37.0
38	34.60063164350545	37.0	37.0	37.0	27.0	37.0
39	34.682871398643286	37.0	37.0	37.0	27.0	37.0
40	34.70509642307505	37.0	37.0	37.0	27.0	37.0
41	34.73911174415572	37.0	37.0	37.0	27.0	37.0
42	34.59234347191852	37.0	37.0	37.0	27.0	37.0
43	34.39639069042303	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	8.0
16	21.0
17	19.0
18	17.0
19	12.0
20	42.0
21	129.0
22	293.0
23	747.0
24	1475.0
25	2293.0
26	3762.0
27	5489.0
28	7333.0
29	9814.0
30	12401.0
31	15523.0
32	19126.0
33	24780.0
34	34776.0
35	61254.0
36	149613.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.06485236336124	20.482846178757406	13.071352215767588	25.380949242113772
2	16.30494281104287	23.047823208599983	36.92391905562991	23.723314924727237
3	18.470413921377006	25.76325978488584	29.961224425459477	25.80510186827768
4	13.01174157641482	17.73244566977425	38.48096041910865	30.774852334702274
5	14.32460859023704	36.896119863239434	35.04990958097733	13.729361965546197
6	30.654198107935382	39.63305066044576	16.651716241893098	13.061034989725762
7	27.23375108545816	31.871344191258444	22.98276736661403	17.91213735666937
8	25.027870839793543	35.91025159702062	20.367063975399148	18.694813587786697
9	25.852675743914986	15.352318939847706	19.986186380688444	38.808818935548864
10	14.609478664836315	28.395871963224824	34.13167646325491	22.86297290868395
11	34.69797753710619	23.227514895495098	23.999873900570602	18.074633666828113
12	23.58775803812215	25.351717101661936	30.1091046653923	20.95142019482362
13	28.348298087587516	22.098925002364364	25.99052534741826	23.56225156262986
14	22.398124557577287	20.482846178757406	26.927673379550686	30.191355884114625
15	23.856292504821873	28.987966102180664	24.019075404592886	23.136665988404584
16	23.4264080864125	28.370365487732528	24.56359566791142	23.639630757943547
17	22.512187223261908	27.346667392693686	26.30176166634664	23.839383717697768
18	23.59549595765352	26.955759161553434	27.10191986381262	22.346825016980436
19	24.676225385534675	26.49520965463086	26.70900550538645	22.119559454448016
20	24.12482697152159	26.758585508309668	26.790110365659686	22.326477154509057
21	23.239838248822835	26.946301704348425	27.278459064972733	22.53540098185601
22	23.08393349974637	26.623888390541396	27.239196288091343	23.052981821620893
23	23.099409338809103	26.797561695578782	27.29536785209683	22.80766111351528
24	23.3470227638129	27.140609461469463	25.772144062866296	23.740223711851343
25	23.501207975215728	27.18503085137176	26.661145040136873	22.652616133275632
26	23.58259942510124	26.513264800204052	26.896435111812938	23.00770066288177
27	23.310912472666516	26.88611788577111	26.47056294797539	23.332406693586986
28	22.985060083512213	26.294310336427547	27.31772184185412	23.40290773820612
29	23.843109382657314	26.445916241319917	26.56169844467817	23.149275931344594
30	23.47856739584617	26.678340416873247	26.837111062072445	23.005981125208137
31	23.402047969369303	26.797275105966513	26.875800659729286	22.924876264934902
32	23.07361627370454	26.542210351043614	27.03055905035666	23.35361432489518
33	23.23869189037374	26.536191969185886	27.336923345876407	22.88819279456397
34	23.58002011859078	26.33013403796166	26.995595117659366	23.094250725788193
35	23.10886679601411	27.119688419773535	26.920222049631587	22.851222734580762
36	23.8485545852905	26.475434971384026	26.735658339327834	22.94035210399764
37	23.54448300666894	26.237852182809785	26.804153256661056	23.413511553860218
38	23.499488437542094	26.533326073063158	26.811317996967883	23.155867492426868
39	23.31033929344197	26.57316202916909	26.797848285191055	23.318650392197885
40	22.948663202753554	27.26670889086954	27.15121327712356	22.63341462925335
41	23.543050058607577	26.07334974536513	26.913917078161585	23.46968311786571
42	23.014865403188594	26.840550137419715	26.968082514881164	23.17650194451052
43	23.500348206378913	25.98078130060098	27.20566530345541	23.313205189564698
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	5.5
2	9.0
3	52.5
4	96.0
5	96.0
6	116.5
7	137.0
8	128.0
9	119.0
10	156.0
11	193.0
12	193.0
13	321.5
14	450.0
15	836.0
16	1222.0
17	1465.0
18	1708.0
19	1708.0
20	1682.5
21	1657.0
22	1957.5
23	2258.0
24	3083.0
25	3908.0
26	3908.0
27	5095.0
28	6282.0
29	7818.0
30	9354.0
31	11187.5
32	13021.0
33	13021.0
34	15343.5
35	17666.0
36	19842.5
37	22019.0
38	23401.5
39	24784.0
40	24784.0
41	25791.5
42	26799.0
43	27913.0
44	29027.0
45	28355.0
46	27683.0
47	27683.0
48	26850.0
49	26017.0
50	26310.5
51	26604.0
52	25299.0
53	23994.0
54	23994.0
55	22595.5
56	21197.0
57	18498.5
58	15800.0
59	15067.0
60	14334.0
61	14334.0
62	13366.0
63	12398.0
64	10064.5
65	7731.0
66	6344.5
67	4958.0
68	4958.0
69	4006.5
70	3055.0
71	2490.0
72	1925.0
73	1534.5
74	1144.0
75	1144.0
76	863.5
77	583.0
78	456.0
79	329.0
80	252.0
81	175.0
82	175.0
83	153.5
84	132.0
85	105.5
86	79.0
87	66.0
88	53.0
89	53.0
90	39.5
91	26.0
92	13.5
93	1.0
94	1.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	348931.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.72187028539707
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.1853731168482	59.50614896457084
2	6.005016440102246	8.013318559563578
3	1.6306348425925008	3.2639701935091714
4	0.7942073905465797	2.119640099670103
5	0.4735323618649018	1.5797482412143837
6	0.3116263656319387	1.247537636712236
7	0.24346755811012138	1.1371227581648122
8	0.16445278728322013	0.8778078032946408
9	0.1357552285563433	0.8152058475270693
>10	0.9242747542801154	12.51958648996559
>50	0.09151390389173422	4.208229085318622
>100	0.0392819115761212	4.248839604856849
>500	8.633387159587077E-4	0.46284471563211477
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	924	0.26480880174017213	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	683	0.19574070518239994	No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG	415	0.11893468909325913	No Hit
CTTTAAAGCCTTAGGCCGTATGACAAAATGAAGAGACTGAAAT	403	0.11549561374598416	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	384	0.11005041111279881	No Hit
ACCCCACACAGGGCACCGAGCCCAATGGAGAAGGTTGGGAGTG	358	0.10259908119370305	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.731792245458271E-4	0.0	0.0
2	2.8658961227291354E-4	0.0	5.731792245458271E-4	0.0	0.0
3	2.8658961227291354E-4	0.0	5.731792245458271E-4	0.0	0.0
4	2.8658961227291354E-4	0.0	5.731792245458271E-4	0.0	0.0
5	2.8658961227291354E-4	0.0	8.597688368187406E-4	0.0	0.0
6	2.8658961227291354E-4	0.0	0.0014329480613645678	0.0	0.0
7	2.8658961227291354E-4	0.0	0.00401225457182079	0.0	0.0
8	2.8658961227291354E-4	0.0	0.004298844184093703	0.0	0.0
9	2.8658961227291354E-4	0.0	0.004872023408639531	5.731792245458271E-4	0.0
10	5.731792245458271E-4	0.0	0.005445202633185358	8.597688368187406E-4	0.0
11	5.731792245458271E-4	0.0	0.008311098755914494	8.597688368187406E-4	0.0
12	5.731792245458271E-4	0.0	0.009457457205006148	8.597688368187406E-4	0.0
13	5.731792245458271E-4	0.0	0.010030636429551975	8.597688368187406E-4	0.0
14	5.731792245458271E-4	0.0	0.010603815654097801	8.597688368187406E-4	0.0
15	5.731792245458271E-4	0.0	0.011750174103189456	8.597688368187406E-4	0.0
16	5.731792245458271E-4	0.0	0.01289653255228111	8.597688368187406E-4	0.0
17	5.731792245458271E-4	0.0	0.01375630138909985	8.597688368187406E-4	0.0
18	5.731792245458271E-4	0.0	0.014329480613645678	8.597688368187406E-4	0.0
19	5.731792245458271E-4	0.0	0.014616070225918591	8.597688368187406E-4	0.0
20	8.597688368187406E-4	0.0	0.014616070225918591	0.0014329480613645678	0.0
21	8.597688368187406E-4	0.0	0.015475839062737333	0.0014329480613645678	0.0
22	8.597688368187406E-4	0.0	0.015762428675010244	0.0017195376736374812	0.0
23	8.597688368187406E-4	0.0	0.015762428675010244	0.002006127285910395	0.0
24	8.597688368187406E-4	0.0	0.015762428675010244	0.002006127285910395	0.0
25	8.597688368187406E-4	0.0	0.015762428675010244	0.002579306510456222	0.0
26	8.597688368187406E-4	0.0	0.015762428675010244	0.0031524857350020493	0.0
27	8.597688368187406E-4	0.0	0.015762428675010244	0.004585433796366617	0.0
28	8.597688368187406E-4	0.0	0.015762428675010244	0.010603815654097801	0.0
29	8.597688368187406E-4	0.0	0.015762428675010244	0.026366244329108047	0.0
30	8.597688368187406E-4	0.0	0.015762428675010244	0.04757387563730365	0.0
31	8.597688368187406E-4	0.0	0.015762428675010244	0.10403202925506762	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTAGTG	25	0.005491163	29.6	17
GGCTGCG	35	8.8569685E-4	26.42857	7
GGTATCA	215	0.0	24.953487	1
CTAGACT	40	0.0019285075	23.125	4
TTCGCCG	40	0.0019285075	23.125	24
GTATCAA	530	0.0	22.688679	1
CTGCGCA	50	2.6968788E-4	22.2	9
AGCCTTA	95	7.1013346E-9	21.421053	7
CTAGTTT	115	1.3460522E-10	20.913044	29
GCCGGCA	45	0.0038205485	20.555557	15
GCCGCTC	45	0.0038205485	20.555557	27
TGTACAC	45	0.0038205485	20.555557	3
CGGAGTC	45	0.0038205485	20.555557	27
GTTCTAG	110	1.7425918E-9	20.181818	1
ATAAAGG	55	5.1332335E-4	20.181818	3
CTCTAGT	110	1.7425918E-9	20.181818	27
CCTTGCA	55	5.1332335E-4	20.181818	9
TCTAGTT	120	2.3646862E-10	20.041668	28
ATAAGCA	65	6.884536E-5	19.923077	5
TAAAGCC	125	4.0927262E-10	19.24	4
>>END_MODULE