FastQCFastQC Report
Fri 10 Feb 2017
ERR1632910.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632910.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281527
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT86033.0558347867167273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70612.5081075704994547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36731.3046705999779773No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG18370.6525129028476843No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17540.6230308283042124No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG13830.49124950715206717No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11920.42340521513034274No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11820.41985315795643047No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11730.4166563064999095No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10370.36834832893470254No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8540.303345682652108No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC7420.26356264230429055No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7420.26356264230429055No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6860.2436711221303818No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT6380.2266212476956029No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6260.2223587790869082No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC6160.2188067219129959No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6100.21667548760864855No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5980.21241301899995382No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5900.209571373260824No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG5890.20921616754343278No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5880.20886096182604155No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA5620.19962561317386965No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT5600.1989152017390872No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5580.1982047903043047No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5520.1960735559999574No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5470.19429752741300124No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5460.19394232169561001No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5460.19394232169561001No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5410.19216629310865388No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT5240.18612779591300302No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5180.18399656160865566No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5160.1832861501738732No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5020.17831327013039602No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT5020.17831327013039602No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC4760.16907792147822412No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4720.1676570986086592No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG4670.16588107002170308No Hit
CTTCCTGAGCCTTTCGGCCCAGGCGGCGGACGGCGACTCGCCG4660.16552586430431185No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4630.16446024715213817No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4460.1584217499564873No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA4420.1570009270869224No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4410.15664572136953117No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4400.15629051565213994No Hit
TATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCGG4380.1555801042173575No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4270.151672841326054No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT4270.151672841326054No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4250.15096242989127154No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4180.14847598986953292No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4150.14741037271735927No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4140.14705516699996804No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4120.14634475556518559No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC4040.14350310982605577No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG4020.14279269839127331No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4010.1424374926738821No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4010.1424374926738821No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3940.13995105265214347No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3840.13639899547823123No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA3810.13533337832605752No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3740.13284693830431896No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3720.1321365268695365No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA3540.1257428239564944No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.12361158965214704No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3460.12290117821736458No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT3430.1218355610651909No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3330.11828350389127863No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3280.11650747530432248No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA3270.11615226958693128No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC3230.11473144671736636No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3220.11437624099997513No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3200.11366582956519268No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3170.112600212413019No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG3150.11188980097823653No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3140.11153459526084533No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3130.1111793895434541No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3130.1111793895434541No Hit
GTTTCATACTAGTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3080.10940336095649796No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT3080.10940336095649796No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3070.10904815523910673No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3060.1086929495217155No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3020.10727212665215058No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2990.10620650949997691No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2930.10407527519562955No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2930.10407527519562955No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA2900.10300965804345585No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG2830.10052321802171728No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTACG251.2312586E-437.02
GAATCAA352.3819524E-531.7142871
CAATAAC352.3819524E-531.7142871
ATTACGC303.5926377E-430.8333323
TGACGCC250.00548938229.614
GGTATCA13500.028.0925941
CTAGTGC405.92445E-527.759
AATAACG405.92445E-527.752
ACGCCAT358.852697E-426.4285726
TCATTAC358.852697E-426.4285721
GACAAGG358.852697E-426.42857235
ATTCGTG358.852697E-426.42857211
TAGTTCC358.852697E-426.4285724
TACGCCA358.852697E-426.4285725
CTAGTTC358.852697E-426.4285723
ACAAGGA358.852697E-426.42857236
GTGGGCG451.3197478E-424.66666620
TCGGGTA551.8960498E-523.54545433
AACGGTC551.8960498E-523.5454545
CCTTGTC551.8960498E-523.54545428
Produced by FastQC (version 0.11.5)