FastQCFastQC Report
Fri 10 Feb 2017
ERR1632909.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632909.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218449
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5380.24628174081822302No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC4650.2128643298893563No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC4410.20187778383055086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4140.18951791951439467No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT4070.18631351024724307No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA3940.1803624644653901No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA3720.17029146391148506No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG3570.16342487262473163No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG3020.13824737123996905No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC3010.13778959848751882No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA2960.1355007347252677No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG2820.12909191619096447No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA2790.1277185979336138No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT2760.1263452796762631No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT2680.12268309765666129No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC2630.12039423389441015No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC2470.11306986985520648No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT2390.10940768783560466No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG2350.10757659682580373No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2330.10666105132090327No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC2280.10437218755865213No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA2270.10391441480620191No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC2210.10116777829150053No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCG250.005486721629.59999815
GTCGTCA250.005486721629.5999981
ATACCGC250.005486721629.5999986
GTATCAA3150.022.317461
TGCTCGC502.6924038E-422.19999910
GGTATCA1350.021.9259261
TCATTGA450.003816041920.5555554
GCCGGCA450.003816041920.55555515
ATACAAT555.1247835E-420.1818186
CTAAGAC656.8694695E-519.9230773
TAATACA701.21346886E-418.54
CGAACTA609.204203E-418.524
ACGGGGA701.21346886E-418.516
TACCGCA609.204203E-418.57
TCGCCGG500.007017097418.49999813
TCGCCGC500.007017097418.49999825
CGCCGCT500.007017097418.49999826
CTAATAC1002.8485192E-718.4999983
AGCACTG500.007017097418.4999985
GCGAACT650.00157440117.07692323
Produced by FastQC (version 0.11.5)