FastQCFastQC Report
Fri 10 Feb 2017
ERR1632908.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632908.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172697
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3700.2142480761101814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3020.17487275401425617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2040.1181259662877757No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC1810.10480784263768333No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTACT303.586932E-430.8333344
TTAACGG250.005483576629.59999835
ATAAAGG250.005483576629.5999983
GCGAGTC250.005483576629.59999820
ATTAGAG405.9113838E-527.753
ATGTTTA358.8387734E-426.42857231
GGTATCA1450.025.517241
GTCCGGC400.001924583823.12515
ACCTTAG450.003812850220.5555551
TGTCCGG450.003812850220.55555514
ATACTTT555.1188044E-420.1818186
CAATACA609.193547E-418.54
GGGTAAA500.00701128618.4999981
GTATCAA2800.017.8392871
GTGTTGA750.004085651614.81
GTATGAA1304.3912532E-614.2307691
TATGAAA1051.6428091E-414.0952382
TAAAGTA800.006270081313.87526
TATAGTA800.006270081313.8752
CTGTGCT800.006270081313.8759
Produced by FastQC (version 0.11.5)