##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632908.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	172697
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.11226020139319	33.0	33.0	33.0	14.0	33.0
2	30.125022438143105	33.0	33.0	33.0	14.0	33.0
3	30.637943913327966	33.0	33.0	33.0	27.0	33.0
4	30.80295546535261	33.0	33.0	33.0	27.0	33.0
5	30.943664336960108	33.0	33.0	33.0	27.0	33.0
6	32.721952321117335	37.0	33.0	37.0	14.0	37.0
7	33.057320046092286	37.0	33.0	37.0	14.0	37.0
8	33.12997330584781	37.0	33.0	37.0	14.0	37.0
9	33.10469782335536	37.0	33.0	37.0	14.0	37.0
10	33.10834004064923	37.0	33.0	37.0	14.0	37.0
11	33.28781623305558	37.0	33.0	37.0	27.0	37.0
12	33.35949090024725	37.0	33.0	37.0	27.0	37.0
13	33.34230473025009	37.0	33.0	37.0	27.0	37.0
14	33.36129753267283	37.0	33.0	37.0	27.0	37.0
15	33.42166337573901	37.0	33.0	37.0	27.0	37.0
16	33.44311713579275	37.0	33.0	37.0	27.0	37.0
17	33.44377725148671	37.0	33.0	37.0	27.0	37.0
18	33.40063232134895	37.0	33.0	37.0	27.0	37.0
19	33.38099098420934	37.0	33.0	37.0	27.0	37.0
20	33.40603484716006	37.0	33.0	37.0	27.0	37.0
21	33.425624069902774	37.0	33.0	37.0	27.0	37.0
22	33.40642280989247	37.0	33.0	37.0	27.0	37.0
23	33.374302969941574	37.0	33.0	37.0	27.0	37.0
24	33.38509065009815	37.0	33.0	37.0	27.0	37.0
25	33.322038020347776	37.0	33.0	37.0	27.0	37.0
26	33.28727771762104	37.0	33.0	37.0	22.0	37.0
27	33.21530773551365	37.0	33.0	37.0	14.0	37.0
28	33.16770412919738	37.0	33.0	37.0	14.0	37.0
29	33.16533581938308	37.0	33.0	37.0	14.0	37.0
30	33.157061211254394	37.0	33.0	37.0	14.0	37.0
31	33.11416527212401	37.0	33.0	37.0	14.0	37.0
32	33.18594995859801	37.0	33.0	37.0	14.0	37.0
33	33.12170448820767	37.0	33.0	37.0	14.0	37.0
34	33.00771872122851	37.0	33.0	37.0	14.0	37.0
35	33.00857571353295	37.0	33.0	37.0	14.0	37.0
36	33.011447795850536	37.0	33.0	37.0	14.0	37.0
37	33.02976311111369	37.0	33.0	37.0	14.0	37.0
38	32.92049659229749	37.0	33.0	37.0	14.0	37.0
39	33.00365379827096	37.0	33.0	37.0	14.0	37.0
40	32.98757361158561	37.0	33.0	37.0	14.0	37.0
41	33.027661163772386	37.0	33.0	37.0	14.0	37.0
42	32.932378674788794	37.0	33.0	37.0	14.0	37.0
43	32.5782845098641	37.0	33.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	8.0
16	22.0
17	17.0
18	24.0
19	40.0
20	137.0
21	329.0
22	688.0
23	1285.0
24	2087.0
25	3045.0
26	4238.0
27	5699.0
28	7233.0
29	8702.0
30	10435.0
31	12693.0
32	14843.0
33	18048.0
34	22961.0
35	31206.0
36	28955.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.59595708089891	22.674395038709417	12.380643554896727	24.349004325494942
2	16.743776672437853	25.43124663427853	34.743510309964854	23.081466383318762
3	18.89320601979189	28.115717123053674	29.823911243391603	23.167165613762833
4	14.630248354053633	20.667990758380284	37.09850200061379	27.603258886952293
5	15.809191821513982	37.037701870906844	34.485833569778286	12.66727273780089
6	29.446371390354205	40.50099306878521	17.262025397082752	12.790610143777831
7	26.419683028657126	32.94266837293062	23.275447749526627	17.36220084888562
8	24.18802874398513	36.80434518260306	20.570710550849174	18.43691552256264
9	25.642599466116955	15.68006392699352	20.39931208996103	38.2780245169285
10	14.75937624857409	28.941440789359397	34.14766903883681	22.151513923229704
11	34.23684256240699	23.76069068947347	24.050215116649394	17.952251631470148
12	22.504733724384327	26.01203263519343	30.88878208654464	20.5944515538776
13	27.673323798328862	22.727088484455432	26.151583409092225	23.448004308123476
14	21.48387059416203	21.339108380574068	27.639159915922107	29.537861109341794
15	23.739265881862455	29.136001204421618	24.230878359207168	22.893854554508763
16	22.885168821693487	28.781044256703936	25.378553188532514	22.95523373307006
17	22.07739566987267	28.12845619784941	26.820963884722953	22.97318424755497
18	22.64775879140923	27.867884213391086	27.932158636224138	21.552198358975545
19	24.201346867635223	27.236141913293228	27.046792937920173	21.51571828115138
20	23.7375287352994	27.139440754616466	27.303311580398038	21.819718929686097
21	22.696977944029136	27.910154779758766	27.412751813870535	21.98011546234156
22	22.674395038709417	27.56735785798248	27.148126487431746	22.61012061587636
23	22.639652107448306	27.25756672090424	27.80766313253849	22.29511803910896
24	22.974342345263672	27.9663225186309	26.39188868364824	22.66744645245719
25	23.37041176164033	27.36816505208545	27.170130343897114	22.09129284237711
26	23.18395803053904	27.664638065513586	27.13596646149036	22.01543744245702
27	23.236072427430702	27.322999241446	26.906084066312673	22.534844264810623
28	22.671499794437658	27.329947827698227	27.48165862753841	22.516893750325714
29	23.534282587421902	27.497871995460255	26.72947416573536	22.23837125138248
30	23.203066642732647	27.02073573947434	27.31662970404813	22.459567913744884
31	23.016612911631356	27.34789834218313	27.41622610699665	22.21926263918887
32	22.394135393203126	27.311418264358966	27.14407314545128	23.150373196986628
33	22.19088924532563	27.52335014505174	27.804767888266735	22.4809927213559
34	23.02819388871839	27.079798722618225	27.49150245806239	22.400504930600995
35	22.53716046022803	27.67506094489192	27.200819933177762	22.58695866170229
36	23.491432972199863	27.11917404471415	26.899135480060455	22.49025750302553
37	22.72361419132932	27.160286513373133	27.284782017058777	22.831317278238767
38	23.003873836835613	27.120332142422853	27.033474814270082	22.84231920647145
39	22.55047858387812	27.174762734731928	27.502504386295072	22.772254295094875
40	22.48272986791896	27.80418883941238	27.53666826870183	22.17641302396683
41	22.6998731883009	26.94024794871943	27.421437546685812	22.938441316293854
42	22.6697626478746	27.385536517715998	27.33805451165915	22.60664632275025
43	23.064673966542557	26.291134182991023	27.471235748160073	23.17295610230635
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	2.0
3	14.0
4	26.0
5	26.0
6	32.5
7	39.0
8	55.5
9	72.0
10	82.0
11	92.0
12	92.0
13	171.5
14	251.0
15	430.5
16	610.0
17	703.5
18	797.0
19	797.0
20	858.0
21	919.0
22	1041.5
23	1164.0
24	1604.0
25	2044.0
26	2044.0
27	2744.0
28	3444.0
29	4301.5
30	5159.0
31	6226.0
32	7293.0
33	7293.0
34	8477.5
35	9662.0
36	10657.5
37	11653.0
38	12632.0
39	13611.0
40	13611.0
41	14210.5
42	14810.0
43	14888.0
44	14966.0
45	14733.5
46	14501.0
47	14501.0
48	13821.0
49	13141.0
50	12839.5
51	12538.0
52	11843.0
53	11148.0
54	11148.0
55	10408.5
56	9669.0
57	8399.0
58	7129.0
59	6474.0
60	5819.0
61	5819.0
62	5062.0
63	4305.0
64	3547.5
65	2790.0
66	2349.5
67	1909.0
68	1909.0
69	1607.0
70	1305.0
71	1026.5
72	748.0
73	595.5
74	443.0
75	443.0
76	352.5
77	262.0
78	201.0
79	140.0
80	115.0
81	90.0
82	90.0
83	79.0
84	68.0
85	54.5
86	41.0
87	30.0
88	19.0
89	19.0
90	16.0
91	13.0
92	8.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	172697.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.34723322551756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.74569390866284	62.43014027153097
2	6.180356642592265	8.695419803066295
3	1.8476536058640365	3.899319573950576
4	0.8467943620296625	2.38278561919016
5	0.5282821835571155	1.858159498778904
6	0.36155072406696653	1.5260455865276183
7	0.2380006713144188	1.1719882112949616
8	0.17326419486075947	0.9750925380401095
9	0.148368135023973	0.9393559017989858
>10	0.858341191598139	11.34717843082481
>50	0.051779274754841204	2.6016060877930305
>100	0.019915105674938927	2.172908477203549
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	370	0.2142480761101814	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	302	0.17487275401425617	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	204	0.1181259662877757	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	181	0.10480784263768333	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	5.790488543518416E-4	0.0	0.0
5	0.0	0.0	5.790488543518416E-4	5.790488543518416E-4	0.0
6	0.0	0.0	0.001737146563055525	5.790488543518416E-4	0.0
7	0.0	0.0	0.004632390834814733	5.790488543518416E-4	0.0
8	0.0	0.0	0.005211439689166575	5.790488543518416E-4	0.0
9	0.0	0.0	0.005790488543518417	5.790488543518416E-4	0.0
10	0.0	0.0	0.006369537397870258	5.790488543518416E-4	0.0
11	0.0	0.0	0.009264781669629466	5.790488543518416E-4	0.0
12	0.0	0.0	0.011001928232684992	0.0011580977087036833	0.0
13	0.0	0.0	0.012160025941388675	0.0011580977087036833	0.0
14	0.0	0.0	0.012739074795740516	0.0011580977087036833	0.0
15	0.0	0.0	0.012739074795740516	0.0011580977087036833	0.0
16	0.0	0.0	0.0138971725044442	0.0011580977087036833	0.0
17	0.0	0.0	0.0138971725044442	0.0011580977087036833	0.0
18	0.0	0.0	0.014476221358796042	0.0011580977087036833	0.0
19	0.0	0.0	0.014476221358796042	0.0011580977087036833	0.0
20	0.0	0.0	0.015055270213147884	0.0011580977087036833	0.0
21	0.0	0.0	0.015055270213147884	0.0011580977087036833	0.0
22	0.0	0.0	0.015634319067499725	0.0011580977087036833	0.0
23	0.0	0.0	0.015634319067499725	0.0011580977087036833	0.0
24	0.0	0.0	0.015634319067499725	0.0011580977087036833	0.0
25	0.0	0.0	0.015634319067499725	0.0011580977087036833	0.0
26	0.0	0.0	0.015634319067499725	0.0011580977087036833	0.0
27	5.790488543518416E-4	0.0	0.015634319067499725	0.001737146563055525	0.0
28	5.790488543518416E-4	0.0	0.015634319067499725	0.004632390834814733	0.0
29	5.790488543518416E-4	0.0	0.016213367921851567	0.012739074795740516	0.0
30	5.790488543518416E-4	0.0	0.016213367921851567	0.03242673584370313	0.0
31	5.790488543518416E-4	0.0	0.016213367921851567	0.08280398617231335	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTACT	30	3.586932E-4	30.833334	4
TTAACGG	25	0.0054835766	29.599998	35
ATAAAGG	25	0.0054835766	29.599998	3
GCGAGTC	25	0.0054835766	29.599998	20
ATTAGAG	40	5.9113838E-5	27.75	3
ATGTTTA	35	8.8387734E-4	26.428572	31
GGTATCA	145	0.0	25.51724	1
GTCCGGC	40	0.0019245838	23.125	15
ACCTTAG	45	0.0038128502	20.555555	1
TGTCCGG	45	0.0038128502	20.555555	14
ATACTTT	55	5.1188044E-4	20.181818	6
CAATACA	60	9.193547E-4	18.5	4
GGGTAAA	50	0.007011286	18.499998	1
GTATCAA	280	0.0	17.839287	1
GTGTTGA	75	0.0040856516	14.8	1
GTATGAA	130	4.3912532E-6	14.230769	1
TATGAAA	105	1.6428091E-4	14.095238	2
TAAAGTA	80	0.0062700813	13.875	26
TATAGTA	80	0.0062700813	13.875	2
CTGTGCT	80	0.0062700813	13.875	9
>>END_MODULE