FastQCFastQC Report
Fri 10 Feb 2017
ERR1632907.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632907.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460065
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8790.19105995891884842No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6500.1412843837283862No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC5540.12041776705465533No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC5030.10933237694673578No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTT250.0054929629.614
GGTATCA2850.025.3157881
TAACCGT1250.023.687
AGCTTCG551.8994444E-523.54545421
GAACGCG400.001929436123.12500235
GTATCAA7600.023.1251
ACCCTAA1350.021.9259243
TTCGCCG603.7200367E-521.58333224
CCGGCAG705.090953E-621.14285916
GCCGGCA705.090953E-621.14285915
TTAACGG705.090953E-621.14285935
AACGGCC806.9414637E-720.812537
TTTAACG909.447285E-820.55555534
CCCCGAC450.003822373520.55555511
TGCTCGC909.447285E-820.55555510
TAGGGGT1001.2847522E-820.354
CTAGAAC656.890633E-519.9230773
TTACTCG656.890633E-519.92307719
GCCGCTC656.890633E-519.92307727
TCGCCGG759.245978E-619.73333413
Produced by FastQC (version 0.11.5)