FastQCFastQC Report
Fri 10 Feb 2017
ERR1632906.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632906.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508079
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40080.7888537018849432No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29590.5823897464764338No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC14770.2907028237734683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14560.2865696082695801No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC14560.2865696082695801No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG13430.2643289724629438No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA13120.2582275591000612No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG12540.24681201151789386No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG12150.23913603986781581No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC12120.23854558051011754No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG11820.2326409869331344No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT11600.22831095164334683No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC11160.21965088106377156No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA10830.21315582812909015No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT10670.2100067115546992No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG10130.19937844311612957No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG9990.19662296611353747No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA9430.185601058103169No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC9420.18540423831726957No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT9420.18540423831726957No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC9080.1787123655966887No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT9020.17753144688129208No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG8840.17398869073510223No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC8410.16552543994142643No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG8250.16237632336703545No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG7950.15647172979005233No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC7820.15391307257335965No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT7730.1521416945002647No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG7730.1521416945002647No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA7610.14977985706947147No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA7390.14544982177968388No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT7340.14446572285018666No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7170.14111978648989626No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT7020.13816748970140472No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT6980.13738021055780697No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA6980.13738021055780697No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC6980.13738021055780697No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG6940.1365929314142092No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC6900.13580565227061145No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT6880.1354120126988126No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT6790.13364063462571765No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG6780.13344381483981824No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC6460.12714558169103624No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC6210.12222508704355033No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA6140.12084734854225425No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC6080.11966642982685763No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG6010.11828869132556158No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT5990.11789505175376272No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC5970.11750141218196382No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5930.11671413303836607No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT5910.1163204934665672No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG5840.11494275496527115No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG5840.11494275496527115No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA5770.11356501646397509No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5770.11356501646397509No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG5720.11258091753447791No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT5490.10805406245879086No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA5490.10805406245879086No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG5470.10766042288699199No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC5440.10706996352929368No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA5390.10608586459979648No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG5390.10608586459979648No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG5320.10470812609850043No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCAT5240.10313356781130494No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA5200.10234628866770717No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT5190.10214946888180775No Hit
CTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGAT5190.10214946888180775No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5190.10214946888180775No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT5110.10057491059461225No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACT251.2326565E-436.9999964
GGTATCA7350.031.2108861
AGACTAT303.5966776E-430.8333326
ATTAGAC303.5966776E-430.8333323
ATTACGC405.933706E-527.753
CATTACG405.933706E-527.752
CGTAATA358.8625547E-426.4285722
CGCTCTC1650.025.78787829
TATTAGA3400.025.573532
CCGCTCT1600.025.437528
AGTGACA2950.025.08474523
ATTAGAG3300.024.106063
CCGGCAG1700.023.94117716
GCTCTCT1700.023.94117730
ACATGTT3350.023.74627129
CAGTGAC3150.023.49206422
GTGACAC3150.023.49206424
CATGTTT3400.023.39705830
GAGGCAC3400.023.3970587
AGCTTCG1750.023.25714121
Produced by FastQC (version 0.11.5)