##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632906.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	508079
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.499066090115907	33.0	33.0	33.0	27.0	33.0
2	31.071628624682383	33.0	33.0	33.0	27.0	33.0
3	31.504704976981927	33.0	33.0	33.0	27.0	33.0
4	31.650863349990846	33.0	33.0	33.0	27.0	33.0
5	31.792858984527996	33.0	33.0	33.0	33.0	33.0
6	34.59923161555585	37.0	37.0	37.0	27.0	37.0
7	34.80286727064098	37.0	37.0	37.0	27.0	37.0
8	34.96274791912281	37.0	37.0	37.0	27.0	37.0
9	35.05816811952472	37.0	37.0	37.0	27.0	37.0
10	35.03939348014777	37.0	37.0	37.0	27.0	37.0
11	35.10328315084859	37.0	37.0	37.0	33.0	37.0
12	35.0106459822193	37.0	37.0	37.0	27.0	37.0
13	35.057416267942585	37.0	37.0	37.0	27.0	37.0
14	35.02332511282694	37.0	37.0	37.0	27.0	37.0
15	35.06213994280417	37.0	37.0	37.0	27.0	37.0
16	35.06255523255242	37.0	37.0	37.0	27.0	37.0
17	35.03736426815515	37.0	37.0	37.0	27.0	37.0
18	35.040936547269226	37.0	37.0	37.0	27.0	37.0
19	35.03155021167968	37.0	37.0	37.0	27.0	37.0
20	35.01266141682691	37.0	37.0	37.0	27.0	37.0
21	35.02886558980001	37.0	37.0	37.0	27.0	37.0
22	35.02780079475829	37.0	37.0	37.0	27.0	37.0
23	35.02660413046003	37.0	37.0	37.0	27.0	37.0
24	35.01641870653973	37.0	37.0	37.0	27.0	37.0
25	35.00898088683059	37.0	37.0	37.0	27.0	37.0
26	34.96614109223172	37.0	37.0	37.0	27.0	37.0
27	34.901464142387304	37.0	37.0	37.0	27.0	37.0
28	34.88900544993987	37.0	37.0	37.0	27.0	37.0
29	34.91085835076829	37.0	37.0	37.0	27.0	37.0
30	34.85866961633919	37.0	37.0	37.0	27.0	37.0
31	34.82401555663588	37.0	37.0	37.0	27.0	37.0
32	34.83706864483673	37.0	37.0	37.0	27.0	37.0
33	34.830719238543615	37.0	37.0	37.0	27.0	37.0
34	34.7629561544563	37.0	37.0	37.0	27.0	37.0
35	34.72664684035357	37.0	37.0	37.0	27.0	37.0
36	34.71359768854843	37.0	37.0	37.0	27.0	37.0
37	34.72009077328526	37.0	37.0	37.0	27.0	37.0
38	34.57081280666983	37.0	37.0	37.0	27.0	37.0
39	34.615280300898085	37.0	37.0	37.0	27.0	37.0
40	34.62949462583575	37.0	37.0	37.0	27.0	37.0
41	34.64265202852312	37.0	37.0	37.0	27.0	37.0
42	34.5204977966025	37.0	37.0	37.0	27.0	37.0
43	34.269989509505415	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	12.0
16	31.0
17	32.0
18	22.0
19	25.0
20	54.0
21	143.0
22	398.0
23	896.0
24	1868.0
25	3200.0
26	5235.0
27	7625.0
28	10616.0
29	13841.0
30	17963.0
31	22274.0
32	28331.0
33	36129.0
34	50287.0
35	88730.0
36	220366.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.36502394312696	21.146120977249602	13.695507982026417	23.793347097597028
2	16.59013657324944	23.141479966698093	38.435164610227936	21.833218849824533
3	16.6373733218653	29.231477781998468	30.30631063279529	23.824838263340936
4	11.369098112695072	18.764601567866414	41.4882331291	28.378067190338513
5	12.683263823145612	37.67839253344461	36.36324272406456	13.27510091934522
6	26.552957315693032	43.39167727853346	16.736373674172718	13.318991731600793
7	26.00756968896569	32.78131944048071	24.069485257213938	17.14162561333966
8	23.57625487374995	35.63697771409564	20.26987929042531	20.516888121729103
9	26.43466862436747	14.468222461467606	20.692845010323197	38.40426390384172
10	16.141977920756418	27.048155897016013	33.83824169076069	22.971624491466876
11	33.26707067208052	24.88274461255041	23.60735239992206	18.242832315447007
12	23.622704343222214	27.837403238472756	28.068076027546894	20.47181639075813
13	27.235134693620484	23.360146748832367	25.694035770027888	23.710682787519264
14	22.260711424798114	21.374825568464747	28.485333973653702	27.879129033083437
15	24.685924826650975	28.038749879447884	26.671442826804494	20.60388246709665
16	22.1603333339894	28.85712654921774	25.71332410904603	23.269216007746827
17	21.947964785003908	26.82988275445354	25.988281349947545	25.233871110595008
18	22.61046018434141	27.23828381019487	28.55264634043131	21.598609665032406
19	25.79283930254941	24.971313516205157	28.495962242092272	20.739884939153164
20	24.44934744399985	25.538744958953234	29.02166789022967	20.990239706817245
21	22.048342875812622	27.852755181772913	28.61956506763712	21.479336874777346
22	22.042831921807434	27.23415059469098	28.502850934598754	22.220166548902828
23	21.857230863704267	27.309335752904566	29.911883781852822	20.921549601538345
24	22.52602449619055	28.454433267267493	26.015442480401667	23.00409975614029
25	22.550626969427984	27.47958486770758	29.117519125962694	20.852269036901742
26	22.2998785621921	27.86810712507307	28.687271073986526	21.144743238748305
27	22.27232379216618	27.320160841129038	27.579569318944497	22.82794604776029
28	21.172494828560126	26.834606429315127	30.121496853835723	21.87140188828903
29	24.5367354289392	26.72970148343073	27.56697285264693	21.166590234983143
30	23.39360611243527	26.113065094207787	28.92247071813635	21.570858075220585
31	22.262876442443005	26.967262965011347	28.855551990930543	21.914308601615105
32	20.670210734944764	26.312246717538017	29.427313468968407	23.59022907854881
33	20.665487060083176	26.57480431192787	30.340950915113595	22.418757712875358
34	22.51677396625328	26.079999370176687	29.868977068526746	21.53424959504329
35	22.067631214830765	27.79154422835819	28.174752351504388	21.966072205306656
36	23.518980316053213	27.55693504356606	27.237299711265372	21.686784929115355
37	21.822787401171865	26.536424453677483	28.27867319845929	23.362114946691364
38	22.4398174299666	26.786582401555663	28.08401843020475	22.689581738272985
39	21.31774783055391	27.15542268033121	28.946679551801985	22.5801499373129
40	21.443909313315448	28.213722669112478	29.45408095985073	20.88828705772134
41	21.976306834173425	26.02646438841204	28.67191913068637	23.325309646728165
42	20.85207221711584	28.46781701270865	28.37176895718973	22.308341812985777
43	22.834637920480873	25.6542781732762	28.473524786499738	23.03755911974319
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	34.0
1	41.5
2	49.0
3	222.0
4	395.0
5	395.0
6	497.0
7	599.0
8	586.0
9	573.0
10	800.0
11	1027.0
12	1027.0
13	1490.5
14	1954.0
15	3483.5
16	5013.0
17	5318.0
18	5623.0
19	5623.0
20	4240.0
21	2857.0
22	2199.0
23	1541.0
24	2027.5
25	2514.0
26	2514.0
27	3538.0
28	4562.0
29	6585.0
30	8608.0
31	12818.5
32	17029.0
33	17029.0
34	22194.5
35	27360.0
36	31236.5
37	35113.0
38	39264.0
39	43415.0
40	43415.0
41	46874.0
42	50333.0
43	51955.0
44	53577.0
45	50596.5
46	47616.0
47	47616.0
48	45064.0
49	42512.0
50	42177.5
51	41843.0
52	37447.0
53	33051.0
54	33051.0
55	32009.5
56	30968.0
57	23915.5
58	16863.0
59	15440.5
60	14018.0
61	14018.0
62	11588.5
63	9159.0
64	6616.0
65	4073.0
66	3365.5
67	2658.0
68	2658.0
69	2055.0
70	1452.0
71	1094.0
72	736.0
73	683.0
74	630.0
75	630.0
76	390.0
77	150.0
78	104.5
79	59.0
80	52.5
81	46.0
82	46.0
83	38.5
84	31.0
85	26.0
86	21.0
87	17.5
88	14.0
89	14.0
90	8.5
91	3.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	508079.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.244749299827596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.9663634900171	20.18730798724368
2	6.87205826198808	3.46966775995396
3	2.7185800556818607	2.0588961587159975
4	1.6721903227535104	1.6885610191804064
5	1.2174994869130957	1.5367734659894912
6	0.8292415671990689	1.256039728376198
7	0.6694324076483875	1.1829757312984472
8	0.5549636602545085	1.120793477891181
9	0.4452070773557154	1.0115226948918585
>10	3.929779577291311	20.572582017358073
>50	0.5938643341734905	10.453500073109161
>100	0.4771151203779157	23.657817803891497
>500	0.04125138887443643	7.026374245599482
>1k	0.01245324947152798	4.777187836500564
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4008	0.7888537018849432	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2959	0.5823897464764338	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1477	0.2907028237734683	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1456	0.2865696082695801	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1456	0.2865696082695801	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	1343	0.2643289724629438	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1312	0.2582275591000612	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1254	0.24681201151789386	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1215	0.23913603986781581	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	1212	0.23854558051011754	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	1182	0.2326409869331344	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	1160	0.22831095164334683	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1116	0.21965088106377156	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	1083	0.21315582812909015	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	1067	0.2100067115546992	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	1013	0.19937844311612957	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	999	0.19662296611353747	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	943	0.185601058103169	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	942	0.18540423831726957	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	942	0.18540423831726957	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	908	0.1787123655966887	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	902	0.17753144688129208	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	884	0.17398869073510223	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	841	0.16552543994142643	No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG	825	0.16237632336703545	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	795	0.15647172979005233	No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC	782	0.15391307257335965	No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT	773	0.1521416945002647	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	773	0.1521416945002647	No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA	761	0.14977985706947147	No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA	739	0.14544982177968388	No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT	734	0.14446572285018666	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	717	0.14111978648989626	No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT	702	0.13816748970140472	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT	698	0.13738021055780697	No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA	698	0.13738021055780697	No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC	698	0.13738021055780697	No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG	694	0.1365929314142092	No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC	690	0.13580565227061145	No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT	688	0.1354120126988126	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	679	0.13364063462571765	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	678	0.13344381483981824	No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC	646	0.12714558169103624	No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC	621	0.12222508704355033	No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA	614	0.12084734854225425	No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC	608	0.11966642982685763	No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG	601	0.11828869132556158	No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT	599	0.11789505175376272	No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC	597	0.11750141218196382	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	593	0.11671413303836607	No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT	591	0.1163204934665672	No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG	584	0.11494275496527115	No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG	584	0.11494275496527115	No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA	577	0.11356501646397509	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	577	0.11356501646397509	No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG	572	0.11258091753447791	No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT	549	0.10805406245879086	No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA	549	0.10805406245879086	No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG	547	0.10766042288699199	No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC	544	0.10706996352929368	No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA	539	0.10608586459979648	No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG	539	0.10608586459979648	No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG	532	0.10470812609850043	No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCAT	524	0.10313356781130494	No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA	520	0.10234628866770717	No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT	519	0.10214946888180775	No Hit
CTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGAT	519	0.10214946888180775	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	519	0.10214946888180775	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	511	0.10057491059461225	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	7.872791435977476E-4	0.0	0.0
2	1.968197858994369E-4	0.0	9.840989294971844E-4	0.0	0.0
3	1.968197858994369E-4	0.0	0.0015745582871954952	0.0	0.0
4	1.968197858994369E-4	0.0	0.0019681978589943688	1.968197858994369E-4	0.0
5	1.968197858994369E-4	0.0	0.0029522967884915534	3.936395717988738E-4	0.0
6	1.968197858994369E-4	0.0	0.005904593576983107	3.936395717988738E-4	0.0
7	1.968197858994369E-4	0.0	0.013186925655262272	5.904593576983107E-4	0.0
8	1.968197858994369E-4	0.0	0.015351943300156079	5.904593576983107E-4	0.0
9	3.936395717988738E-4	0.0	0.019485158804044254	5.904593576983107E-4	0.0
10	7.872791435977476E-4	0.0	0.021059717091239747	5.904593576983107E-4	0.0
11	7.872791435977476E-4	0.0	0.03424664274650202	7.872791435977476E-4	0.0
12	7.872791435977476E-4	0.0	0.03759257910679245	7.872791435977476E-4	0.0
13	7.872791435977476E-4	0.0	0.041528974824781184	7.872791435977476E-4	0.0
14	7.872791435977476E-4	0.0	0.04507173097097105	7.872791435977476E-4	0.0
15	9.840989294971844E-4	0.0	0.0495985860466581	7.872791435977476E-4	0.0
16	9.840989294971844E-4	0.0	0.05392862133644571	9.840989294971844E-4	0.0
17	9.840989294971844E-4	0.0	0.05530635983774177	0.0011809187153966215	0.0
18	0.0011809187153966215	0.0	0.0570777379108367	0.0013777385012960584	0.0
19	0.0011809187153966215	0.0	0.05845547641213276	0.0013777385012960584	0.0
20	0.0011809187153966215	0.0	0.06062049405702657	0.0015745582871954952	0.0
21	0.0011809187153966215	0.0	0.06376961063141756	0.0017713780730949321	0.0
22	0.0011809187153966215	0.0	0.06455688977501531	0.002165017644893806	0.0
23	0.0011809187153966215	0.0	0.06534416891861305	0.0035427561461898642	0.0
24	0.0011809187153966215	0.0	0.06554098870451248	0.004133215503888175	0.0
25	0.0011809187153966215	0.0	0.06573780849041193	0.004723674861586486	0.0
26	0.0011809187153966215	0.0	0.06573780849041193	0.004920494647485923	0.0
27	0.0011809187153966215	0.0	0.06593462827631136	0.0055109540051842335	0.0
28	0.0011809187153966215	0.0	0.06593462827631136	0.020272437947642	0.0
29	0.0011809187153966215	0.0	0.06593462827631136	0.05471590048004346	0.0
30	0.0011809187153966215	0.0	0.06593462827631136	0.09112756087143928	0.0
31	0.0011809187153966215	0.0	0.06593462827631136	0.20547985647901212	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGACT	25	1.2326565E-4	36.999996	4
GGTATCA	735	0.0	31.210886	1
AGACTAT	30	3.5966776E-4	30.833332	6
ATTAGAC	30	3.5966776E-4	30.833332	3
ATTACGC	40	5.933706E-5	27.75	3
CATTACG	40	5.933706E-5	27.75	2
CGTAATA	35	8.8625547E-4	26.428572	2
CGCTCTC	165	0.0	25.787878	29
TATTAGA	340	0.0	25.57353	2
CCGCTCT	160	0.0	25.4375	28
AGTGACA	295	0.0	25.084745	23
ATTAGAG	330	0.0	24.10606	3
CCGGCAG	170	0.0	23.941177	16
GCTCTCT	170	0.0	23.941177	30
ACATGTT	335	0.0	23.746271	29
CAGTGAC	315	0.0	23.492064	22
GTGACAC	315	0.0	23.492064	24
CATGTTT	340	0.0	23.397058	30
GAGGCAC	340	0.0	23.397058	7
AGCTTCG	175	0.0	23.257141	21
>>END_MODULE