FastQCFastQC Report
Fri 10 Feb 2017
ERR1632905.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632905.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321096
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10550.32856217455215886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7890.24572090589730175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4940.15384807035902034No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGCG250.005490517229.637
GTATCAA6250.023.681
CGGTTGG400.001928172923.12500233
GGTATCA2150.022.3720931
CTTAAGC450.003819894720.5555553
ACGGGCT450.003819894720.55555516
CGAACCG656.8823465E-519.92307731
GCGAACC656.8823465E-519.92307730
GAATAGT851.2395976E-619.5882366
CACGAGG500.007024111718.514
ACGGGTA500.007024111718.516
GAACCGG701.2157279E-418.532
GGCGAAC701.2157279E-418.529
TGTAATA701.2157279E-418.52
TATCGGT609.217066E-418.52
TCTATAC609.217066E-418.53
CTATTTA650.001576583317.0769234
ATCAACG8900.016.4213473
AACCGGG803.3730757E-416.187533
ACCGGGG803.3730757E-416.187534
Produced by FastQC (version 0.11.5)