##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632905.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	321096
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.868042579166357	33.0	33.0	33.0	27.0	33.0
2	30.641029474051376	33.0	33.0	33.0	27.0	33.0
3	31.083622966340286	33.0	33.0	33.0	27.0	33.0
4	31.259645090564817	33.0	33.0	33.0	27.0	33.0
5	31.37425255998206	33.0	33.0	33.0	27.0	33.0
6	33.92520616887162	37.0	33.0	37.0	27.0	37.0
7	34.144807160475374	37.0	33.0	37.0	27.0	37.0
8	34.279474051374045	37.0	37.0	37.0	27.0	37.0
9	34.36007611430849	37.0	37.0	37.0	27.0	37.0
10	34.38472917756683	37.0	37.0	37.0	27.0	37.0
11	34.43938261454518	37.0	37.0	37.0	27.0	37.0
12	34.439239355208414	37.0	37.0	37.0	27.0	37.0
13	34.43517515011087	37.0	37.0	37.0	27.0	37.0
14	34.44911802077883	37.0	37.0	37.0	27.0	37.0
15	34.44100518225079	37.0	37.0	37.0	27.0	37.0
16	34.4697722799412	37.0	37.0	37.0	27.0	37.0
17	34.437903306176345	37.0	37.0	37.0	27.0	37.0
18	34.407996362458576	37.0	37.0	37.0	27.0	37.0
19	34.41739542068416	37.0	37.0	37.0	27.0	37.0
20	34.41087089219423	37.0	37.0	37.0	27.0	37.0
21	34.41728641901487	37.0	37.0	37.0	27.0	37.0
22	34.423163166155916	37.0	37.0	37.0	27.0	37.0
23	34.3795500411092	37.0	37.0	37.0	27.0	37.0
24	34.38635485960585	37.0	37.0	37.0	27.0	37.0
25	34.370527817226	37.0	37.0	37.0	27.0	37.0
26	34.325914991155294	37.0	37.0	37.0	27.0	37.0
27	34.2611025985998	37.0	37.0	37.0	27.0	37.0
28	34.2158388768468	37.0	37.0	37.0	27.0	37.0
29	34.24605725390538	37.0	37.0	37.0	27.0	37.0
30	34.21054139571966	37.0	37.0	37.0	27.0	37.0
31	34.18458654109674	37.0	37.0	37.0	27.0	37.0
32	34.25496424745248	37.0	37.0	37.0	27.0	37.0
33	34.19354336397837	37.0	37.0	37.0	27.0	37.0
34	34.11465729875177	37.0	37.0	37.0	27.0	37.0
35	34.101670528440096	37.0	37.0	37.0	27.0	37.0
36	34.09024092483245	37.0	37.0	37.0	27.0	37.0
37	34.1082666865984	37.0	37.0	37.0	27.0	37.0
38	33.96724966988066	37.0	33.0	37.0	27.0	37.0
39	34.02055460049331	37.0	33.0	37.0	27.0	37.0
40	34.06890774098712	37.0	33.0	37.0	27.0	37.0
41	34.0676277499564	37.0	33.0	37.0	27.0	37.0
42	33.987713954705136	37.0	33.0	37.0	27.0	37.0
43	33.706246729949925	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	12.0
16	27.0
17	25.0
18	19.0
19	39.0
20	86.0
21	235.0
22	547.0
23	1151.0
24	2145.0
25	3463.0
26	4965.0
27	6937.0
28	9194.0
29	11521.0
30	14376.0
31	17536.0
32	21433.0
33	26590.0
34	36880.0
35	59953.0
36	103961.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.68409447641827	21.175598574881032	13.423711288835738	24.71659565986496
2	16.101415153100632	24.343809950918104	37.36764083015671	22.18713406582455
3	18.236913571019258	27.643134763435235	30.83190073996562	23.28805092557989
4	13.53209009143683	19.123564292298877	38.525238557939055	28.819107058325237
5	14.165856939980568	37.22002142661385	35.44173705060169	13.172384582803897
6	28.753394623414803	40.49412013852555	17.75419189276727	12.998293345292375
7	25.671761716122283	32.95992475770486	23.760183870244415	17.608129655928444
8	23.76205247028926	36.867167451478686	20.936417769140693	18.434362309091362
9	25.64528988215362	15.802127712584399	20.34718588833246	38.20539651692952
10	14.408774945810599	28.885442359917285	34.512419961631416	22.193362732640708
11	33.33021899992525	24.270934549169095	24.364364551411416	18.03448189949423
12	22.2092458330219	26.08596805939657	31.06547574557142	20.639310362010114
13	27.801031467224757	22.762974312978052	26.832162343971895	22.603831875825296
14	21.836460124075042	21.433776814410642	28.28811321224805	28.441649849266266
15	23.427261628920945	29.174141066846055	24.858297829932482	22.54029947430052
16	22.414480404614196	29.37781847173431	25.433203777063557	22.774497346587935
17	22.098686997035156	28.132085108503375	26.855519844532477	22.913708049928992
18	22.59386600891945	27.693586964646087	27.976679871440318	21.735867154994146
19	24.28681764955029	26.860502777985403	28.092221640879988	20.760457931584323
20	24.084385978025264	26.5101402695767	28.06138974014002	21.344084012258016
21	22.572688541744526	27.729401798838975	27.515447093704065	22.182462565712434
22	22.31482173555572	27.52821586067718	27.510464160251143	22.646498243515957
23	22.314510302214913	27.54472182773999	27.871726835588113	22.269041034456986
24	22.676084410892692	27.713830131798588	27.335127189376387	22.274958267932334
25	22.85110994842664	27.293706555048956	27.790131300296483	22.065052196227917
26	22.73183097889728	27.454094725564943	27.59984552906296	22.214228766474825
27	22.610994842663874	27.33824152278446	27.437277325161325	22.61348630939034
28	21.986259561003564	27.4646834591524	28.085681540723023	22.463375439121013
29	23.00900665221616	27.517938560430526	27.404888257717317	22.068166529635995
30	22.66580711064604	27.48617235966814	27.659952163838852	22.188068365846974
31	22.759237112888357	27.359107556618582	27.63939756334554	22.242257767147517
32	21.80220245658619	27.285297854847144	27.909410269825845	23.003089418740814
33	22.14851633156439	27.276266287963725	28.108416174601984	22.466801205869896
34	22.247240700600443	27.101240750429778	27.926227670229466	22.725290878740314
35	22.505418940130053	27.717255898547478	27.729401798838975	22.047923362483495
36	22.79038044696913	27.351944589780004	27.554687694645843	22.30298726860503
37	22.842078381543214	27.083489050003738	27.508284126865483	22.566148441587565
38	22.874778882328027	27.420459924757708	27.377170690385427	22.32759050252884
39	22.338802102797917	27.322669855744074	27.751202132695518	22.587325908762487
40	22.235406233649748	27.493335326506713	27.97885990482597	22.292398535017565
41	22.250977900690135	27.0174651817525	28.0685527069786	22.66300421057877
42	22.496698806587435	26.97479881406184	27.799474300520714	22.72902807883001
43	22.355308069860726	26.727209308119694	27.974499838054662	22.942982783964922
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	9.5
2	9.0
3	54.0
4	99.0
5	99.0
6	130.0
7	161.0
8	180.0
9	199.0
10	249.5
11	300.0
12	300.0
13	461.5
14	623.0
15	1152.5
16	1682.0
17	1929.0
18	2176.0
19	2176.0
20	2261.0
21	2346.0
22	2710.5
23	3075.0
24	4081.0
25	5087.0
26	5087.0
27	6462.0
28	7837.0
29	9904.5
30	11972.0
31	13520.0
32	15068.0
33	15068.0
34	16617.0
35	18166.0
36	19520.0
37	20874.0
38	22477.0
39	24080.0
40	24080.0
41	24788.5
42	25497.0
43	24859.5
44	24222.0
45	24168.0
46	24114.0
47	24114.0
48	23238.5
49	22363.0
50	22637.5
51	22912.0
52	22658.5
53	22405.0
54	22405.0
55	20210.5
56	18016.0
57	16106.0
58	14196.0
59	12371.0
60	10546.0
61	10546.0
62	9323.0
63	8100.0
64	6630.5
65	5161.0
66	4374.0
67	3587.0
68	3587.0
69	2952.0
70	2317.0
71	1956.5
72	1596.0
73	1330.5
74	1065.0
75	1065.0
76	796.5
77	528.0
78	419.0
79	310.0
80	229.0
81	148.0
82	148.0
83	124.0
84	100.0
85	89.0
86	78.0
87	59.0
88	40.0
89	40.0
90	34.5
91	29.0
92	15.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	321096.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.98243978173956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.51473980543928	64.95939507381158
2	4.671694370079973	6.632165286057869
3	1.1934728277096582	2.541468393721297
4	0.6055344138212757	1.7192924025935867
5	0.3562562167851475	1.2643967727411045
6	0.23674252179302005	1.0082737078170128
7	0.19321799386053048	0.960055922976754
8	0.14466237969102286	0.8214790924080938
9	0.10506466687212593	0.6711971750495285
>10	0.874884554613006	12.429907866808442
>50	0.07592171440689627	3.640468134049889
>100	0.026925724295463544	2.774137607270217
>500	4.4140531631907447E-4	0.2472096873883218
>1k	4.4140531631907447E-4	0.33055287730631117
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1055	0.32856217455215886	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	789	0.24572090589730175	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	494	0.15384807035902034	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.114333408077335E-4	0.0	0.0
2	0.0	0.0	3.114333408077335E-4	0.0	0.0
3	0.0	0.0	6.22866681615467E-4	0.0	0.0
4	0.0	0.0	9.343000224232006E-4	0.0	0.0
5	0.0	0.0	9.343000224232006E-4	0.0	0.0
6	0.0	0.0	0.0015571667040386677	0.0	0.0
7	0.0	0.0	0.004360066771308269	0.0	0.0
8	0.0	0.0	0.004982933452923736	0.0	0.0
9	0.0	0.0	0.005605800134539203	3.114333408077335E-4	0.0
10	0.0	0.0	0.006228666816154671	3.114333408077335E-4	0.0
11	0.0	0.0	0.01152303360988614	3.114333408077335E-4	0.0
12	0.0	0.0	0.013080200313924808	3.114333408077335E-4	0.0
13	0.0	0.0	0.01339163365473254	3.114333408077335E-4	0.0
14	0.0	0.0	0.014014500336348008	3.114333408077335E-4	0.0
15	0.0	0.0	0.01588310038119441	3.114333408077335E-4	0.0
16	0.0	0.0	0.01775170042604081	3.114333408077335E-4	0.0
17	0.0	0.0	0.01775170042604081	3.114333408077335E-4	0.0
18	0.0	0.0	0.01868600044846401	3.114333408077335E-4	0.0
19	0.0	0.0	0.01962030047088721	3.114333408077335E-4	0.0
20	0.0	0.0	0.02024316715250268	6.22866681615467E-4	0.0
21	0.0	0.0	0.02024316715250268	9.343000224232006E-4	0.0
22	0.0	0.0	0.020554600493310413	0.0015571667040386677	0.0
23	0.0	0.0	0.020554600493310413	0.0015571667040386677	0.0
24	0.0	0.0	0.020554600493310413	0.001868600044846401	0.0
25	0.0	0.0	0.020866033834118145	0.0021800333856541346	0.0
26	0.0	0.0	0.020866033834118145	0.0028029000672696014	0.0
27	0.0	0.0	0.020866033834118145	0.0034257667488850688	0.0
28	0.0	0.0	0.020866033834118145	0.010277300246655206	0.0
29	0.0	0.0	0.020866033834118145	0.024603233923810947	0.0
30	0.0	0.0	0.020866033834118145	0.045469267757929095	0.0
31	0.0	0.0	0.020866033834118145	0.1124274360315918	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGGGCG	25	0.0054905172	29.6	37
GTATCAA	625	0.0	23.68	1
CGGTTGG	40	0.0019281729	23.125002	33
GGTATCA	215	0.0	22.372093	1
CTTAAGC	45	0.0038198947	20.555555	3
ACGGGCT	45	0.0038198947	20.555555	16
CGAACCG	65	6.8823465E-5	19.923077	31
GCGAACC	65	6.8823465E-5	19.923077	30
GAATAGT	85	1.2395976E-6	19.588236	6
CACGAGG	50	0.0070241117	18.5	14
ACGGGTA	50	0.0070241117	18.5	16
GAACCGG	70	1.2157279E-4	18.5	32
GGCGAAC	70	1.2157279E-4	18.5	29
TGTAATA	70	1.2157279E-4	18.5	2
TATCGGT	60	9.217066E-4	18.5	2
TCTATAC	60	9.217066E-4	18.5	3
CTATTTA	65	0.0015765833	17.076923	4
ATCAACG	890	0.0	16.421347	3
AACCGGG	80	3.3730757E-4	16.1875	33
ACCGGGG	80	3.3730757E-4	16.1875	34
>>END_MODULE