FastQCFastQC Report
Fri 10 Feb 2017
ERR1632904.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632904.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379355
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7370.19427712828353388No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5460.14392851023447695No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4490.1183587932147988No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4370.1151955292536015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1750.024.3142871
TAACCTA400.001928815723.1255
TTAACGG903.807145E-922.61111335
GTATCAA5050.022.3465351
AACGGCC1001.2829332E-820.34999837
CTATCGG555.134368E-420.18181812
ATCGGTC555.134368E-420.18181814
TAGACTG656.886562E-519.9230775
TAACGGC1052.2475433E-819.3809536
AACTAGA1153.0304363E-919.30434827
GTCGCCC902.1444903E-618.537
CATGCTA1501.2732926E-1118.54
GTTTAAC1103.8313374E-818.533
TGTGTAA801.6120224E-518.510
TAACCGT1002.8623253E-718.4999987
ACTAGAA1258.525603E-917.7628
TTTAACG1156.371374E-817.69565234
AACCGTG1054.776357E-717.6190478
AGGTCGC953.593519E-617.52631635
CTCTAGC752.0626702E-417.2666662
Produced by FastQC (version 0.11.5)