##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632904.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	379355
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24448867156094	33.0	33.0	33.0	27.0	33.0
2	30.902948425617165	33.0	33.0	33.0	27.0	33.0
3	31.361310645701256	33.0	33.0	33.0	27.0	33.0
4	31.514612961474082	33.0	33.0	33.0	27.0	33.0
5	31.637714014577373	33.0	33.0	33.0	27.0	33.0
6	34.40428622266742	37.0	33.0	37.0	27.0	37.0
7	34.60797669728882	37.0	37.0	37.0	27.0	37.0
8	34.74340129957428	37.0	37.0	37.0	27.0	37.0
9	34.84725125542038	37.0	37.0	37.0	27.0	37.0
10	34.86092446389266	37.0	37.0	37.0	27.0	37.0
11	34.91912588472539	37.0	37.0	37.0	27.0	37.0
12	34.867949545939815	37.0	37.0	37.0	27.0	37.0
13	34.89635565631137	37.0	37.0	37.0	27.0	37.0
14	34.87671442316564	37.0	37.0	37.0	27.0	37.0
15	34.9099102423851	37.0	37.0	37.0	27.0	37.0
16	34.90153286499453	37.0	37.0	37.0	27.0	37.0
17	34.86990813354246	37.0	37.0	37.0	27.0	37.0
18	34.85286868500481	37.0	37.0	37.0	27.0	37.0
19	34.86440932635658	37.0	37.0	37.0	27.0	37.0
20	34.85887361442448	37.0	37.0	37.0	27.0	37.0
21	34.874626668951244	37.0	37.0	37.0	27.0	37.0
22	34.85075984236401	37.0	37.0	37.0	27.0	37.0
23	34.84415389279171	37.0	37.0	37.0	27.0	37.0
24	34.83775619143019	37.0	37.0	37.0	27.0	37.0
25	34.82631308405056	37.0	37.0	37.0	27.0	37.0
26	34.78357475188148	37.0	37.0	37.0	27.0	37.0
27	34.720707516705986	37.0	37.0	37.0	27.0	37.0
28	34.69453941558698	37.0	37.0	37.0	27.0	37.0
29	34.73717230562402	37.0	37.0	37.0	27.0	37.0
30	34.695989244902535	37.0	37.0	37.0	27.0	37.0
31	34.672728710574525	37.0	37.0	37.0	27.0	37.0
32	34.70324102753358	37.0	37.0	37.0	27.0	37.0
33	34.68062105415771	37.0	37.0	37.0	27.0	37.0
34	34.61976776370418	37.0	37.0	37.0	27.0	37.0
35	34.62176061999973	37.0	37.0	37.0	27.0	37.0
36	34.57451990879255	37.0	37.0	37.0	27.0	37.0
37	34.61378919481752	37.0	37.0	37.0	27.0	37.0
38	34.493115946804444	37.0	37.0	37.0	27.0	37.0
39	34.556397042348195	37.0	37.0	37.0	27.0	37.0
40	34.58535145180635	37.0	37.0	37.0	27.0	37.0
41	34.616757390834444	37.0	37.0	37.0	27.0	37.0
42	34.51751789221178	37.0	37.0	37.0	27.0	37.0
43	34.27283678875987	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	13.0
16	22.0
17	19.0
18	14.0
19	31.0
20	42.0
21	143.0
22	366.0
23	873.0
24	1652.0
25	2913.0
26	4360.0
27	6300.0
28	8753.0
29	11249.0
30	14052.0
31	17623.0
32	21787.0
33	28084.0
34	39205.0
35	68127.0
36	153725.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.6990813354246	20.745475873522164	12.977290400811906	25.578152390241332
2	16.39651513753608	22.951588881127176	36.97038394116329	23.681512040173452
3	17.958113113047148	26.79442738332169	29.950574000606295	25.296885503024868
4	12.949084630491228	18.192721856835945	38.683027770821525	30.175165741851302
5	14.108420872270036	37.03813051099893	35.559568214469294	13.293880402261735
6	29.995386906723255	39.84763611920233	17.224763084709576	12.932213889364844
7	26.85110252929314	31.957137773325776	23.759802823213086	17.431956874167994
8	24.448076340103597	36.44422770228414	20.434157978674328	18.673537978937933
9	25.70863702864072	15.526353942876725	20.170025437914354	38.5949835905682
10	14.670691041372857	28.15990299323852	34.34645648535014	22.82294948003849
11	34.35805511987453	23.272923778518802	24.07243874471142	18.296582356895254
12	22.927337190757996	25.648798618708067	30.470403711563048	20.953460478970882
13	27.78927389911824	22.064556945341433	26.096664074547586	24.049505080992738
14	21.631189782657405	20.77130919587194	27.610285879980495	29.987215141490157
15	23.850746662097507	28.809953737264564	23.990721092380486	23.348578508257436
16	23.18250715029458	28.33915461770637	25.101817558751037	23.376520673248013
17	22.611538005298467	27.6076498266795	26.347089138142373	23.433723029879662
18	22.825585533339485	27.572853923106326	27.477165188280107	22.124395355274086
19	24.450712393404594	26.578271012639874	26.899342304701403	22.07167428925413
20	24.004428569545677	26.710600888349962	27.142650024383492	22.14232051772087
21	23.108697657866642	27.235702705908714	27.06066876672246	22.594930869502182
22	22.70195463352269	27.2546822896759	26.95127255473106	23.092090522070357
23	22.774973309960327	26.72536278683555	27.588933848242412	22.91073005496171
24	23.185670414255778	27.47294750299851	26.050796747110226	23.290585335635487
25	23.315364236664866	26.808662071147076	27.094146643645132	22.781827048542922
26	23.211240131275453	27.210396594219134	27.000039540799513	22.578323733705897
27	23.13558540153682	27.246774129772906	26.346561927482178	23.271078541208105
28	22.685083892396303	26.833440972176458	27.56969065914513	22.91178447628211
29	24.208195489712804	26.833440972176458	26.473356091260165	22.485007446850574
30	23.47220940807423	26.614121337533447	27.03984394564458	22.87382530874774
31	23.12372316168233	26.9011875420121	27.031672180411487	22.943417115894082
32	22.438612908753015	26.70084749113627	27.14818573631559	23.712353863795126
33	22.46681867907369	26.845303212030945	27.68383176707833	23.00404634181703
34	23.280831938421795	26.53451252784331	27.390966245337477	22.793689288397413
35	23.144020772100014	27.176127901306163	26.954699424022355	22.72515190257147
36	23.67465830159086	26.930974944313373	26.38794796430784	23.00641878978793
37	22.823213085368586	26.74355155461243	26.884053195555612	23.549182164463364
38	23.210185709955056	26.85004810797274	26.816833836380173	23.12293234569203
39	22.844301511776568	26.795745409972188	27.12367044061631	23.236282637634933
40	22.79790697367901	27.209605778228834	27.452122681920628	22.54036456617153
41	22.71539850535778	26.26853474977264	27.52329612104757	23.49277062382201
42	22.78235425920312	26.95759908265345	27.195371090403448	23.06467556773998
43	23.202013944721962	26.0829565973824	27.28816016659857	23.42686929129707
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6.0
1	9.5
2	13.0
3	47.0
4	81.0
5	81.0
6	115.0
7	149.0
8	133.0
9	117.0
10	171.0
11	225.0
12	225.0
13	329.5
14	434.0
15	766.5
16	1099.0
17	1311.5
18	1524.0
19	1524.0
20	1549.0
21	1574.0
22	1971.5
23	2369.0
24	3333.5
25	4298.0
26	4298.0
27	5759.0
28	7220.0
29	9083.5
30	10947.0
31	13362.0
32	15777.0
33	15777.0
34	18222.0
35	20667.0
36	22658.5
37	24650.0
38	26707.5
39	28765.0
40	28765.0
41	30051.5
42	31338.0
43	31610.0
44	31882.0
45	30924.5
46	29967.0
47	29967.0
48	28850.5
49	27734.0
50	27776.0
51	27818.0
52	26148.5
53	24479.0
54	24479.0
55	23405.0
56	22331.0
57	19715.5
58	17100.0
59	15823.5
60	14547.0
61	14547.0
62	12875.0
63	11203.0
64	9347.0
65	7491.0
66	6289.5
67	5088.0
68	5088.0
69	4221.5
70	3355.0
71	2759.5
72	2164.0
73	1721.0
74	1278.0
75	1278.0
76	987.0
77	696.0
78	553.5
79	411.0
80	322.5
81	234.0
82	234.0
83	187.5
84	141.0
85	120.0
86	99.0
87	77.0
88	55.0
89	55.0
90	38.5
91	22.0
92	14.5
93	7.0
94	3.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	379355.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.3612120630958
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.73254708293209	63.13690774263914
2	5.928356508143538	8.342526989102433
3	1.644585326667874	3.47145050776602
4	0.7574106208410549	2.131693172473541
5	0.42613997401415626	1.4991862540086094
6	0.27621311447175	1.1660813713172975
7	0.19729018899384876	0.9717103778035111
8	0.13774371785192296	0.775345195371071
9	0.09904530280356061	0.6272052798973379
>10	0.6881151586636656	9.174536553015509
>50	0.07378056490069958	3.551530982067334
>100	0.03801957680878747	4.812007970706384
>500	7.528629071047022E-4	0.33981760383180815
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	737	0.19427712828353388	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	546	0.14392851023447695	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	449	0.1183587932147988	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	437	0.1151955292536015	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.6360533009977464E-4	0.0	0.0
2	0.0	0.0	2.6360533009977464E-4	0.0	0.0
3	0.0	0.0	2.6360533009977464E-4	0.0	0.0
4	0.0	0.0	5.272106601995493E-4	2.6360533009977464E-4	0.0
5	0.0	0.0	7.908159902993238E-4	2.6360533009977464E-4	0.0
6	0.0	0.0	0.001318026650498873	2.6360533009977464E-4	0.0
7	0.0	0.0	0.002636053300997746	2.6360533009977464E-4	0.0
8	0.0	0.0	0.002636053300997746	2.6360533009977464E-4	0.0
9	0.0	0.0	0.00342686929129707	2.6360533009977464E-4	0.0
10	7.908159902993238E-4	0.0	0.0039540799514966194	2.6360533009977464E-4	0.0
11	7.908159902993238E-4	0.0	0.005272106601995492	2.6360533009977464E-4	0.0
12	7.908159902993238E-4	0.0	0.005799317262195042	2.6360533009977464E-4	0.0
13	7.908159902993238E-4	0.0	0.0065901332524943654	2.6360533009977464E-4	0.0
14	7.908159902993238E-4	0.0	0.00685373858259414	5.272106601995493E-4	0.0
15	7.908159902993238E-4	0.0	0.00685373858259414	5.272106601995493E-4	0.0
16	7.908159902993238E-4	0.0	0.007908159902993239	5.272106601995493E-4	0.0
17	7.908159902993238E-4	0.0	0.008171765233093013	5.272106601995493E-4	0.0
18	7.908159902993238E-4	0.0	0.008698975893292563	5.272106601995493E-4	0.0
19	7.908159902993238E-4	0.0	0.008698975893292563	5.272106601995493E-4	0.0
20	7.908159902993238E-4	0.0	0.008962581223392337	5.272106601995493E-4	0.0
21	7.908159902993238E-4	0.0	0.009226186553492112	0.0010544213203990986	0.0
22	7.908159902993238E-4	0.0	0.009489791883591886	0.0010544213203990986	0.0
23	7.908159902993238E-4	0.0	0.00975339721369166	0.0010544213203990986	0.0
24	7.908159902993238E-4	0.0	0.01028060787389121	0.001318026650498873	0.0
25	7.908159902993238E-4	0.0	0.01028060787389121	0.001318026650498873	0.0
26	7.908159902993238E-4	0.0	0.01028060787389121	0.0015816319805986476	0.0
27	7.908159902993238E-4	0.0	0.01028060787389121	0.002899658631097521	0.0
28	7.908159902993238E-4	0.0	0.01028060787389121	0.007908159902993239	0.0
29	7.908159902993238E-4	0.0	0.01028060787389121	0.019243189097283547	0.0
30	7.908159902993238E-4	0.0	0.01028060787389121	0.037431956874168	0.0
31	7.908159902993238E-4	0.0	0.01028060787389121	0.09990642010781459	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	175	0.0	24.314287	1
TAACCTA	40	0.0019288157	23.125	5
TTAACGG	90	3.807145E-9	22.611113	35
GTATCAA	505	0.0	22.346535	1
AACGGCC	100	1.2829332E-8	20.349998	37
CTATCGG	55	5.134368E-4	20.181818	12
ATCGGTC	55	5.134368E-4	20.181818	14
TAGACTG	65	6.886562E-5	19.923077	5
TAACGGC	105	2.2475433E-8	19.38095	36
AACTAGA	115	3.0304363E-9	19.304348	27
GTCGCCC	90	2.1444903E-6	18.5	37
CATGCTA	150	1.2732926E-11	18.5	4
GTTTAAC	110	3.8313374E-8	18.5	33
TGTGTAA	80	1.6120224E-5	18.5	10
TAACCGT	100	2.8623253E-7	18.499998	7
ACTAGAA	125	8.525603E-9	17.76	28
TTTAACG	115	6.371374E-8	17.695652	34
AACCGTG	105	4.776357E-7	17.619047	8
AGGTCGC	95	3.593519E-6	17.526316	35
CTCTAGC	75	2.0626702E-4	17.266666	2
>>END_MODULE