FastQCFastQC Report
Fri 10 Feb 2017
ERR1632903.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632903.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393351
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13630.3465098601503492No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9160.2328708964766837No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT8770.22295608756555851No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC8300.21100747169830505No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC7930.20160111452621193No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA7620.19372011257121502No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG7190.18278840018202572No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA6390.16245033062074332No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC6290.15990807192558298No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT5940.15101016649252194No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA5760.1464341008412334No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC5500.13982422823381663No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC5390.13702774366914028No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5350.13601084019107618No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT5150.13092632280075556No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG5110.12990941932269146No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA4890.12431645019333878No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA4770.12126573975914641No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC4330.11007980150044107No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC4270.1085544462833449No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG4180.10626641345770063No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG4060.10321570302350826No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT4010.1019445736759281No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTAC250.00549200529.59999824
GGTATCA1900.028.2368431
GTATCAA7250.026.537931
CCCGTAG400.001928941723.12527
CGTAGGG400.001928941723.12529
CTCTAAT652.6754024E-622.769231
CACGATA502.697727E-422.19999916
GAATTAT502.697727E-422.1999991
TTAGAGG450.003821401620.5555574
AACGGAA450.003821401620.55555733
ACGGAAC450.003821401620.55555734
TCTAATA656.887387E-519.9230772
TCGCCAT1103.8324288E-818.513
CAGTTGT701.2166122E-418.527
TATACAG609.222099E-418.55
AATACTG1205.142283E-918.55
TGCGGGT500.00702686118.49999821
GCACCGC500.00702686118.49999810
GGCTTAC500.00702686118.4999981
ACAGCGC500.00702686118.4999988
Produced by FastQC (version 0.11.5)