##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632903.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	393351
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50460784388498	33.0	33.0	33.0	27.0	33.0
2	31.094907601607723	33.0	33.0	33.0	27.0	33.0
3	31.54338237350356	33.0	33.0	33.0	27.0	33.0
4	31.677356864479815	33.0	33.0	33.0	27.0	33.0
5	31.78232418374429	33.0	33.0	33.0	33.0	33.0
6	34.62305930326858	37.0	37.0	37.0	27.0	37.0
7	34.78407071546786	37.0	37.0	37.0	27.0	37.0
8	34.97029879166444	37.0	37.0	37.0	27.0	37.0
9	35.011213903104355	37.0	37.0	37.0	27.0	37.0
10	35.09640244972048	37.0	37.0	37.0	33.0	37.0
11	35.13826073913629	37.0	37.0	37.0	33.0	37.0
12	35.05033926442287	37.0	37.0	37.0	33.0	37.0
13	35.102908089721396	37.0	37.0	37.0	33.0	37.0
14	35.045061535371715	37.0	37.0	37.0	27.0	37.0
15	35.11384488662797	37.0	37.0	37.0	33.0	37.0
16	35.100236175832784	37.0	37.0	37.0	33.0	37.0
17	35.082353928171024	37.0	37.0	37.0	33.0	37.0
18	35.036133122834315	37.0	37.0	37.0	27.0	37.0
19	35.07776006670887	37.0	37.0	37.0	33.0	37.0
20	35.006673429074795	37.0	37.0	37.0	27.0	37.0
21	35.064283553365826	37.0	37.0	37.0	33.0	37.0
22	35.0854122653813	37.0	37.0	37.0	33.0	37.0
23	35.05940241667111	37.0	37.0	37.0	33.0	37.0
24	35.04471578818917	37.0	37.0	37.0	27.0	37.0
25	35.0598854458232	37.0	37.0	37.0	33.0	37.0
26	34.9941172133794	37.0	37.0	37.0	27.0	37.0
27	34.93111750065463	37.0	37.0	37.0	27.0	37.0
28	34.9279828956835	37.0	37.0	37.0	27.0	37.0
29	34.97405370775719	37.0	37.0	37.0	27.0	37.0
30	34.94506433185628	37.0	37.0	37.0	27.0	37.0
31	34.926083828438216	37.0	37.0	37.0	27.0	37.0
32	34.955978248434604	37.0	37.0	37.0	27.0	37.0
33	34.919738350735095	37.0	37.0	37.0	27.0	37.0
34	34.855892065864836	37.0	37.0	37.0	27.0	37.0
35	34.863831539769826	37.0	37.0	37.0	27.0	37.0
36	34.839519919868	37.0	37.0	37.0	27.0	37.0
37	34.85886396627948	37.0	37.0	37.0	27.0	37.0
38	34.71666272616569	37.0	37.0	37.0	27.0	37.0
39	34.76962560156196	37.0	37.0	37.0	27.0	37.0
40	34.82113176272591	37.0	37.0	37.0	27.0	37.0
41	34.836713266268546	37.0	37.0	37.0	27.0	37.0
42	34.700821912236144	37.0	37.0	37.0	27.0	37.0
43	34.50632132624552	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	9.0
16	20.0
17	15.0
18	18.0
19	20.0
20	44.0
21	122.0
22	330.0
23	710.0
24	1406.0
25	2498.0
26	3828.0
27	5587.0
28	7858.0
29	10218.0
30	13347.0
31	16707.0
32	20763.0
33	27184.0
34	38467.0
35	68884.0
36	175312.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.48140210651556	20.65432654296036	14.268426926587196	24.595844423936892
2	15.528370335908642	23.872826051033304	38.831730439226035	21.76707317383202
3	17.933347061530284	27.10047769040882	31.610953067362228	23.355222180698664
4	13.22940579787518	18.616451972919858	38.36548019453364	29.788662034671326
5	13.615320667800516	37.930753957661224	35.193249794712614	13.260675579825651
6	28.740997226395766	39.804398615994366	17.907416022839655	13.547188134770218
7	25.304880374017095	33.0394482281728	23.764525830619473	17.891145567190627
8	23.2797170974524	37.05570851478705	21.06159638592504	18.60297800183551
9	25.38623265226223	16.52061390462971	19.998423799609	38.094729643499065
10	14.619258626519319	29.047593625032096	33.99050720603227	22.342640542416316
11	32.872167606031255	24.389158792020357	24.737448233257318	18.001225368691067
12	22.111295001156726	26.356612796204914	30.296859547833872	21.235232654804488
13	27.446479098820138	22.678981367786022	27.219201171472807	22.655338361921032
14	21.819443702952327	21.756141461442834	27.737821945285507	28.686592890319336
15	23.725883498453033	29.016323843081626	25.114719423619107	22.14307323484623
16	22.63500029235975	29.29266736324555	24.94998106017272	23.122351284221978
17	22.456787957829015	28.048485957834096	26.045948783656325	23.44877730068056
18	22.098075255941893	27.811038995706127	27.830868613528377	22.260017134823606
19	24.693975609570078	26.891504025666645	27.99357317001864	20.42094719474464
20	24.43313986744663	26.003238837577637	28.187547508459364	21.37607378651637
21	22.263067845257797	28.181191861721466	26.96980559347758	22.585934699543156
22	21.98494474400726	27.709094422030194	27.066157198024154	23.239803635938387
23	22.084601284857545	27.85578274874095	27.591896296183304	22.4677196702182
24	23.54741693805278	27.800869960925485	26.634481671585934	22.017231429435796
25	23.364882763740273	26.9479421686992	27.58274416488073	22.104430902679795
26	23.089556147054413	27.206235652127493	26.949467523916297	22.7547406769018
27	22.82211053232355	27.116239694318818	27.012007087817242	23.049642685540395
28	21.643519401247232	27.709348647899713	27.899763824167216	22.747368126685835
29	23.10735195792053	27.78078611723372	26.91667238674873	22.19518953809702
30	22.361961708499535	27.84841019852498	27.39436279556935	22.395265297406134
31	23.082183596838448	27.14623834692171	27.53037363576043	22.24120442047942
32	21.953674962056787	27.341483814710017	27.60511604139814	23.099725181835055
33	22.235357225480552	27.08242765367318	27.989251330236865	22.692963790609404
34	22.144852815932843	27.145475669313157	27.550711705321714	23.15895980943229
35	22.62076364366685	27.761210725280982	27.02929444694433	22.588731184107832
36	22.71101382734504	27.75892269245534	26.95150133087243	22.57856214932719
37	23.5936860463047	26.591263273768213	26.995736632168217	22.81931404775887
38	23.404287773515257	27.184880679088142	27.052937452809324	22.357894094587277
39	22.67211726930909	27.110646725189465	27.527577151195754	22.689658854305698
40	22.65686371713813	27.02573528477111	27.641470340738934	22.67593065735183
41	22.368825806976467	27.147001024530255	27.854257393523852	22.62991577496943
42	22.945918530777856	26.85896311436859	27.36385569122743	22.831262663626127
43	22.489837320866098	26.73185017961058	27.575371614664768	23.20294088485856
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8.0
1	12.0
2	16.0
3	63.0
4	110.0
5	110.0
6	156.0
7	202.0
8	193.5
9	185.0
10	265.5
11	346.0
12	346.0
13	504.5
14	663.0
15	1342.0
16	2021.0
17	2343.0
18	2665.0
19	2665.0
20	2620.0
21	2575.0
22	3109.5
23	3644.0
24	4573.5
25	5503.0
26	5503.0
27	6997.5
28	8492.0
29	11715.5
30	14939.0
31	15864.0
32	16789.0
33	16789.0
34	18560.0
35	20331.0
36	22211.5
37	24092.0
38	26223.0
39	28354.0
40	28354.0
41	30896.5
42	33439.0
43	31162.0
44	28885.0
45	29645.5
46	30406.0
47	30406.0
48	30324.5
49	30243.0
50	30942.5
51	31642.0
52	31857.5
53	32073.0
54	32073.0
55	26848.0
56	21623.0
57	19024.5
58	16426.0
59	14097.5
60	11769.0
61	11769.0
62	10374.0
63	8979.0
64	7364.5
65	5750.0
66	4875.5
67	4001.0
68	4001.0
69	3315.0
70	2629.0
71	2250.0
72	1871.0
73	1536.5
74	1202.0
75	1202.0
76	915.5
77	629.0
78	487.0
79	345.0
80	268.0
81	191.0
82	191.0
83	164.0
84	137.0
85	118.0
86	99.0
87	72.0
88	45.0
89	45.0
90	37.5
91	30.0
92	16.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	393351.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.621606161592496
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.95844204138267	49.48011427974638
2	5.75942758985658	6.40697226238425
3	1.6399536412468712	2.73650566670109
4	0.7919769443782279	1.7620411875706894
5	0.47281937209745745	1.3149486450188117
6	0.33193940734700383	1.1077801790980482
7	0.23607908643017406	0.9191768577885389
8	0.1820631360934901	0.8101315241869217
9	0.15136998841033858	0.7577497692040205
>10	1.184163623246499	13.569398410251738
>50	0.15994078012670843	6.410621263690036
>100	0.12444959836947769	11.815232615813098
>500	0.006913866576082095	2.5598901546867547
>1k	4.6092443840547293E-4	0.3494371838595941
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1363	0.3465098601503492	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	916	0.2328708964766837	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	877	0.22295608756555851	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	830	0.21100747169830505	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	793	0.20160111452621193	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	762	0.19372011257121502	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	719	0.18278840018202572	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	639	0.16245033062074332	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	629	0.15990807192558298	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	594	0.15101016649252194	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	576	0.1464341008412334	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	550	0.13982422823381663	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	539	0.13702774366914028	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	535	0.13601084019107618	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	515	0.13092632280075556	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	511	0.12990941932269146	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	489	0.12431645019333878	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	477	0.12126573975914641	No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC	433	0.11007980150044107	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	427	0.1085544462833449	No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG	418	0.10626641345770063	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	406	0.10321570302350826	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	401	0.1019445736759281	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	7.626776085480906E-4	0.0	0.0
2	0.0	0.0	7.626776085480906E-4	0.0	0.0
3	0.0	0.0	7.626776085480906E-4	0.0	0.0
4	0.0	0.0	7.626776085480906E-4	0.0	0.0
5	0.0	0.0	0.0010169034780641208	0.0	0.0
6	0.0	0.0	0.0020338069561282417	0.0	0.0
7	0.0	0.0	0.005592969129352665	0.0	0.0
8	0.0	0.0	0.006864098476932816	0.0	0.0
9	0.0	0.0	0.008389453694028997	2.542258695160302E-4	0.0
10	5.084517390320604E-4	2.542258695160302E-4	0.008897905433061057	2.542258695160302E-4	0.0
11	5.084517390320604E-4	2.542258695160302E-4	0.01321974521483357	5.084517390320604E-4	0.0
12	5.084517390320604E-4	2.542258695160302E-4	0.014236648692897692	5.084517390320604E-4	0.0
13	5.084517390320604E-4	2.542258695160302E-4	0.015507778040477843	5.084517390320604E-4	0.0
14	5.084517390320604E-4	2.542258695160302E-4	0.016524681518541962	5.084517390320604E-4	0.0
15	5.084517390320604E-4	2.542258695160302E-4	0.017795810866122114	7.626776085480906E-4	0.0
16	5.084517390320604E-4	2.542258695160302E-4	0.018304262605154175	7.626776085480906E-4	0.0
17	5.084517390320604E-4	2.542258695160302E-4	0.018558488474670204	7.626776085480906E-4	0.0
18	5.084517390320604E-4	2.542258695160302E-4	0.018558488474670204	7.626776085480906E-4	0.0
19	5.084517390320604E-4	2.542258695160302E-4	0.018812714344186236	7.626776085480906E-4	0.0
20	5.084517390320604E-4	2.542258695160302E-4	0.019321166083218298	7.626776085480906E-4	0.0
21	5.084517390320604E-4	2.542258695160302E-4	0.020338069561282417	0.0010169034780641208	0.0
22	5.084517390320604E-4	2.542258695160302E-4	0.020592295430798446	0.001271129347580151	0.0
23	5.084517390320604E-4	2.542258695160302E-4	0.020592295430798446	0.0015253552170961812	0.0
24	5.084517390320604E-4	2.542258695160302E-4	0.020592295430798446	0.0017795810866122115	0.0
25	5.084517390320604E-4	2.542258695160302E-4	0.020592295430798446	0.002288032825644272	0.0
26	5.084517390320604E-4	2.542258695160302E-4	0.020592295430798446	0.0030507104341923625	0.0
27	5.084517390320604E-4	2.542258695160302E-4	0.020846521300314478	0.0033049363037083927	0.0
28	5.084517390320604E-4	2.542258695160302E-4	0.020846521300314478	0.01067748651967327	0.0
29	5.084517390320604E-4	2.542258695160302E-4	0.020846521300314478	0.021863424778378597	0.0
30	5.084517390320604E-4	2.542258695160302E-4	0.020846521300314478	0.039405009774984685	0.0
31	5.084517390320604E-4	2.542258695160302E-4	0.020846521300314478	0.09838541150270369	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGTAC	25	0.005492005	29.599998	24
GGTATCA	190	0.0	28.236843	1
GTATCAA	725	0.0	26.53793	1
CCCGTAG	40	0.0019289417	23.125	27
CGTAGGG	40	0.0019289417	23.125	29
CTCTAAT	65	2.6754024E-6	22.76923	1
CACGATA	50	2.697727E-4	22.199999	16
GAATTAT	50	2.697727E-4	22.199999	1
TTAGAGG	45	0.0038214016	20.555557	4
AACGGAA	45	0.0038214016	20.555557	33
ACGGAAC	45	0.0038214016	20.555557	34
TCTAATA	65	6.887387E-5	19.923077	2
TCGCCAT	110	3.8324288E-8	18.5	13
CAGTTGT	70	1.2166122E-4	18.5	27
TATACAG	60	9.222099E-4	18.5	5
AATACTG	120	5.142283E-9	18.5	5
TGCGGGT	50	0.007026861	18.499998	21
GCACCGC	50	0.007026861	18.499998	10
GGCTTAC	50	0.007026861	18.499998	1
ACAGCGC	50	0.007026861	18.499998	8
>>END_MODULE