FastQCFastQC Report
Fri 10 Feb 2017
ERR1632902.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632902.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427001
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT81901.9180282950157026No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT63581.488989487144058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33770.7908646583965846No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17470.4091325313055473No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG14690.34402729735995935No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG10940.25620548898011947No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9750.22833670178758364No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9720.2276341273205449No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9520.22295029754028678No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9110.21334844649075763No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7590.17775134016079588No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7130.1669785316662022No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC6680.1564399146606214No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6330.1482432125451697No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6290.14730644658911804No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6090.14262261680885993No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6070.14215423383083411No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC6010.14074908489675667No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG5930.13887555298465343No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC5860.1372362125615631No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA5750.1346601061824211No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5720.1339575317153824No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC5690.1332549572483437No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC5470.12810274449005973No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG5400.12646340406696938No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG5400.12646340406696938No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT5340.12505825513289195No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5320.12458987215486614No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG5210.12201376577572418No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA5160.12084280833065965No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5100.11943765939658221No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5040.11803251046250476No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5000.11709574450645314No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4820.11288029770422084No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4760.11147514877014339No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4740.11100676579211759No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4700.11006999983606597No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4630.10843065941297562No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA4600.10772808494593689No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4590.10749389345692398No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4570.10702551047889818No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4550.10655712750087237No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC4520.10585455303383365No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC4400.10304425516567876No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4380.10257587218765296No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4340.10163910623160134No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT4330.10140491474258842No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG4280.10023395729752389No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCAA352.3848193E-531.7142851
GCACGAA250.005492524729.59999811
TTGACGC250.005492524729.59999826
GGACAGC250.005492524729.5999986
GGTATCA11300.026.685841
CGGATCG358.860231E-426.4285737
CACTAGT358.860231E-426.428572
GGCAGCT3900.025.14102618
CCTAGTG601.3341196E-624.66666817
GCCGGCA3900.024.66666815
GCAGCTT4150.024.51807219
CCGGCAG3850.024.50649316
CGCTCTC4350.024.2413829
CGGCAGC4050.024.20987717
CCGCTCT4400.023.96590828
GCCGCTC4400.023.96590827
AGCTTCG4350.023.81609321
CCGTTTA701.917033E-723.78571327
GCTCTCT4450.023.69662930
GCTTCGC4450.023.69662922
Produced by FastQC (version 0.11.5)