##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632902.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	427001
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.548825412586854	33.0	33.0	33.0	27.0	33.0
2	31.08091784328374	33.0	33.0	33.0	27.0	33.0
3	31.5316146800593	33.0	33.0	33.0	27.0	33.0
4	31.64774789754591	33.0	33.0	33.0	27.0	33.0
5	31.7895086896752	33.0	33.0	33.0	33.0	33.0
6	34.55403617321739	37.0	33.0	37.0	27.0	37.0
7	34.735351907840965	37.0	37.0	37.0	27.0	37.0
8	34.97004925047014	37.0	37.0	37.0	27.0	37.0
9	35.02552218847263	37.0	37.0	37.0	27.0	37.0
10	35.03322006271648	37.0	37.0	37.0	27.0	37.0
11	35.069597026704855	37.0	37.0	37.0	27.0	37.0
12	34.96949187472629	37.0	37.0	37.0	27.0	37.0
13	35.052316036730595	37.0	37.0	37.0	27.0	37.0
14	34.97221552174351	37.0	37.0	37.0	27.0	37.0
15	35.033681419949836	37.0	37.0	37.0	27.0	37.0
16	35.034095938885386	37.0	37.0	37.0	27.0	37.0
17	35.03317088250379	37.0	37.0	37.0	27.0	37.0
18	35.026803215917525	37.0	37.0	37.0	27.0	37.0
19	35.0437258929136	37.0	37.0	37.0	27.0	37.0
20	34.94715234858935	37.0	37.0	37.0	27.0	37.0
21	35.01319669040588	37.0	37.0	37.0	27.0	37.0
22	35.000681497233025	37.0	37.0	37.0	27.0	37.0
23	35.01899527167384	37.0	37.0	37.0	27.0	37.0
24	35.01753860061218	37.0	37.0	37.0	27.0	37.0
25	34.990180350865685	37.0	37.0	37.0	27.0	37.0
26	34.955274109428316	37.0	37.0	37.0	27.0	37.0
27	34.8486092538425	37.0	37.0	37.0	27.0	37.0
28	34.849314170224424	37.0	37.0	37.0	27.0	37.0
29	34.88299090634448	37.0	37.0	37.0	27.0	37.0
30	34.841904351512056	37.0	37.0	37.0	27.0	37.0
31	34.79503092498612	37.0	37.0	37.0	27.0	37.0
32	34.79462577371013	37.0	37.0	37.0	27.0	37.0
33	34.74233783995822	37.0	37.0	37.0	27.0	37.0
34	34.66024201348475	37.0	37.0	37.0	27.0	37.0
35	34.61656061695406	37.0	37.0	37.0	27.0	37.0
36	34.564207109585226	37.0	37.0	37.0	27.0	37.0
37	34.53749522834841	37.0	37.0	37.0	27.0	37.0
38	34.330469952061	37.0	37.0	37.0	27.0	37.0
39	34.365746216051015	37.0	37.0	37.0	27.0	37.0
40	34.33989381757888	37.0	37.0	37.0	27.0	37.0
41	34.28052393319922	37.0	37.0	37.0	27.0	37.0
42	34.04687576844082	37.0	37.0	37.0	27.0	37.0
43	33.81489973091398	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	12.0
16	23.0
17	33.0
18	21.0
19	37.0
20	53.0
21	139.0
22	378.0
23	862.0
24	1602.0
25	2785.0
26	4533.0
27	6701.0
28	9410.0
29	12234.0
30	15907.0
31	19647.0
32	24516.0
33	31505.0
34	43016.0
35	73892.0
36	179694.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.41886552958893	19.02759009932061	14.123151936412325	22.43039243467814
2	16.5941063369875	24.20322200650584	37.99405622000885	21.20861543649781
3	18.251947887709864	29.251922126646075	30.421006039798502	22.075123945845558
4	11.414961557467079	18.334383291842407	40.560560748101295	29.690094402589224
5	13.961794000482433	36.73504277507546	33.41350488640542	15.88965833803668
6	25.809073046667336	42.638073447134786	16.19410727375346	15.358746232444421
7	25.214929239041595	33.670881332830604	22.39502951983719	18.719159908290614
8	24.9954918138365	33.25565982281072	19.657096821787302	22.091751541565475
9	27.933658234992425	13.17818927824525	19.481921588005648	39.406230898756675
10	16.990124144908325	26.67066353474582	31.52732663389547	24.81188568645038
11	34.47439233163389	24.610012622921257	21.911892478003566	19.003702567441294
12	24.31258943187487	27.439514193175192	27.461528193142403	20.78636818180754
13	29.991733040437847	22.83929077449467	25.408137217477243	21.76083896759024
14	23.559663794698373	21.253580202388285	29.49993091351074	25.6868250894026
15	26.153100344027298	26.476752981843134	27.224526406261347	20.145620267868225
16	20.861309458291664	28.18143283036808	26.55146006683825	24.405797644502
17	21.903929967377124	25.2966620687071	26.03319430165269	26.766213662263088
18	24.170669389533046	23.372310603488046	29.67557453027042	22.781445476708488
19	26.798063704768843	23.330624518443752	30.186814550785595	19.684497226001813
20	25.363640834564787	22.921960370116228	30.44512776316683	21.269271032152147
21	22.334842307160873	25.918206280547352	30.311872805918487	21.43507860637329
22	22.770672668213894	25.13647509022227	30.06948461479013	22.02336762677371
23	21.874890222739527	25.665982046880455	31.412572804279147	21.046554926100875
24	22.354514392237956	25.8519300891567	29.56597291341238	22.22758260519296
25	22.343741583743363	25.741860089320635	31.890089250376462	20.02430907655954
26	22.23296900944026	26.07979840796626	30.42990531638099	21.257327266212492
27	22.779103561818356	25.412586855768488	30.306720593160204	21.501588989252955
28	21.335547223542804	25.487996515230645	30.987749443209733	22.188706818016822
29	23.315636263146924	25.671836834105775	29.131313509804425	21.881213392942875
30	23.34186570991637	25.09197870730982	30.68938948620729	20.876766096566516
31	22.301352924232027	25.289870515525724	30.406017784501678	22.00275877574057
32	20.188477310357587	25.321486366542466	31.206015910969764	23.284020412130182
33	20.485432118425955	25.49736417479116	31.591729293374023	22.425474413408867
34	21.490581989269348	25.352633834581184	31.135992655754908	22.020791520394567
35	21.3481935639495	26.55356779023937	30.305315444226128	21.79292320158501
36	22.15474905210995	26.203685705654085	30.079320657328672	21.562244584907294
37	22.032032711867185	25.887761386975676	30.00156908297639	22.07863681818075
38	21.661541776248768	26.199938641829878	29.274404509591314	22.86411507233004
39	21.0107236282819	25.822421961541075	30.22006974222543	22.946784667951597
40	21.143041819574194	27.14911674679919	30.788686677548764	20.919154756077855
41	21.964351371542456	25.48307849396137	29.736698508902787	22.81587162559338
42	20.611427139514895	27.471364235680944	29.82194421090349	22.09526441390067
43	21.78355554202449	25.985887620872084	29.52897065814834	22.701586178955086
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17.0
1	59.5
2	102.0
3	426.5
4	751.0
5	751.0
6	1055.0
7	1359.0
8	1300.5
9	1242.0
10	1761.5
11	2281.0
12	2281.0
13	3580.0
14	4879.0
15	7946.5
16	11014.0
17	11434.0
18	11854.0
19	11854.0
20	9117.5
21	6381.0
22	4478.5
23	2576.0
24	2462.5
25	2349.0
26	2349.0
27	2745.0
28	3141.0
29	3954.0
30	4767.0
31	6690.0
32	8613.0
33	8613.0
34	11956.5
35	15300.0
36	16810.0
37	18320.0
38	20927.5
39	23535.0
40	23535.0
41	26439.0
42	29343.0
43	31932.0
44	34521.0
45	36070.0
46	37619.0
47	37619.0
48	38396.0
49	39173.0
50	40598.0
51	42023.0
52	39487.0
53	36951.0
54	36951.0
55	33110.0
56	29269.0
57	24299.5
58	19330.0
59	17703.5
60	16077.0
61	16077.0
62	13027.5
63	9978.0
64	7210.0
65	4442.0
66	4357.0
67	4272.0
68	4272.0
69	3401.5
70	2531.0
71	1961.5
72	1392.0
73	1347.0
74	1302.0
75	1302.0
76	751.5
77	201.0
78	128.5
79	56.0
80	39.0
81	22.0
82	22.0
83	15.0
84	8.0
85	7.5
86	7.0
87	4.5
88	2.0
89	2.0
90	1.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	427001.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.90518390787962
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.71020562726112	17.381508374994205
2	9.831857260106757	4.70064711917747
3	4.241180661921029	3.0415861112929448
4	2.5073690616573154	2.3975647417538273
5	1.5741645493789287	1.8815346527083867
6	1.200585162094228	1.7220125458160391
7	0.8539924682206635	1.4290392906131304
8	0.7141573425566949	1.3657650090384303
9	0.5763199725709977	1.2399331440684453
>10	4.606564486513387	22.382070629996363
>50	0.6742044727512673	11.172530631940338
>100	0.4774366488520085	21.996934454567782
>500	0.0259693574689377	3.9798200669597703
>1k	0.0039952857644519535	1.8354257772210076
>5k	0.0019976428822259767	3.473627449851856
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8190	1.9180282950157026	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6358	1.488989487144058	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3377	0.7908646583965846	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1747	0.4091325313055473	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	1469	0.34402729735995935	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	1094	0.25620548898011947	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	975	0.22833670178758364	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	972	0.2276341273205449	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	952	0.22295029754028678	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	911	0.21334844649075763	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	759	0.17775134016079588	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	713	0.1669785316662022	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	668	0.1564399146606214	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	633	0.1482432125451697	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	629	0.14730644658911804	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	609	0.14262261680885993	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	607	0.14215423383083411	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	601	0.14074908489675667	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	593	0.13887555298465343	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	586	0.1372362125615631	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	575	0.1346601061824211	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	572	0.1339575317153824	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	569	0.1332549572483437	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	547	0.12810274449005973	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	540	0.12646340406696938	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	540	0.12646340406696938	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	534	0.12505825513289195	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	532	0.12458987215486614	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	521	0.12201376577572418	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	516	0.12084280833065965	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	510	0.11943765939658221	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	504	0.11803251046250476	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	500	0.11709574450645314	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	482	0.11288029770422084	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	476	0.11147514877014339	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	474	0.11100676579211759	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	470	0.11006999983606597	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	463	0.10843065941297562	No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA	460	0.10772808494593689	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	459	0.10749389345692398	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	457	0.10702551047889818	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	455	0.10655712750087237	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	452	0.10585455303383365	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	440	0.10304425516567876	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	438	0.10257587218765296	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	434	0.10163910623160134	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	433	0.10140491474258842	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	428	0.10023395729752389	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.003044489357167782	0.0	0.0
2	4.6838297802581256E-4	0.0	0.0037470638242065005	0.0	0.0
3	4.6838297802581256E-4	0.0	0.007494127648413001	0.0	0.0
4	4.6838297802581256E-4	0.0	0.00819670211545172	2.3419148901290628E-4	0.0
5	7.025744670387189E-4	0.0	0.011709574450645315	9.367659560516251E-4	0.0
6	7.025744670387189E-4	0.0	0.02341914890129063	9.367659560516251E-4	0.0
7	9.367659560516251E-4	0.0	0.0484776382256716	0.0014051489340774377	0.0
8	0.0011709574450645315	0.0	0.05854787225322657	0.0014051489340774377	0.0
9	0.0014051489340774377	0.0	0.07166259563794933	0.0018735319121032502	0.0
10	0.0063231702033484695	0.0	0.08079606370945266	0.0018735319121032502	0.0
11	0.006791553181374282	0.0	0.1154564040833628	0.0025761063791419694	0.0
12	0.006791553181374282	0.0	0.12786855300104683	0.0028102978681548755	0.0
13	0.006791553181374282	0.0	0.1381729785176147	0.0028102978681548755	0.0
14	0.006791553181374282	0.0	0.14730644658911807	0.0028102978681548755	0.0
15	0.006791553181374282	0.0	0.16159212741890533	0.003044489357167782	0.0
16	0.006791553181374282	0.0	0.17751714867178298	0.003278680846180688	0.0
17	0.006791553181374282	0.0	0.18220097845204108	0.003278680846180688	0.0
18	0.007025744670387189	0.0	0.18735319121032504	0.0037470638242065005	0.0
19	0.007025744670387189	0.0	0.1934421699246606	0.0037470638242065005	0.0
20	0.007025744670387189	0.0	0.19765761672689292	0.004215446802232313	0.0
21	0.007025744670387189	0.0	0.20351240395221556	0.0044496382912452195	0.0
22	0.007025744670387189	0.0	0.20632270182037044	0.0053864042472968445	0.0
23	0.007025744670387189	0.0	0.20749365926543498	0.007259936159400095	0.0
24	0.007025744670387189	0.0	0.20936719117753821	0.008899276582490439	0.0
25	0.007025744670387189	0.0	0.21030395713358985	0.009836042538542065	0.0
26	0.007025744670387189	0.0	0.21077234011161566	0.011241191472619502	0.0
27	0.007025744670387189	0.0	0.21100653160062857	0.01639340423090344	0.0
28	0.007025744670387189	0.0	0.21124072308964148	0.042622851000348946	0.0
29	0.007025744670387189	0.0	0.21124072308964148	0.10725970196791108	0.0
30	0.007259936159400095	0.0	0.21124072308964148	0.17962487207289912	0.0
31	0.007494127648413001	0.0	0.21124072308964148	0.35222399947541105	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAATCAA	35	2.3848193E-5	31.714285	1
GCACGAA	25	0.0054925247	29.599998	11
TTGACGC	25	0.0054925247	29.599998	26
GGACAGC	25	0.0054925247	29.599998	6
GGTATCA	1130	0.0	26.68584	1
CGGATCG	35	8.860231E-4	26.42857	37
CACTAGT	35	8.860231E-4	26.42857	2
GGCAGCT	390	0.0	25.141026	18
CCTAGTG	60	1.3341196E-6	24.666668	17
GCCGGCA	390	0.0	24.666668	15
GCAGCTT	415	0.0	24.518072	19
CCGGCAG	385	0.0	24.506493	16
CGCTCTC	435	0.0	24.24138	29
CGGCAGC	405	0.0	24.209877	17
CCGCTCT	440	0.0	23.965908	28
GCCGCTC	440	0.0	23.965908	27
AGCTTCG	435	0.0	23.816093	21
CCGTTTA	70	1.917033E-7	23.785713	27
GCTCTCT	445	0.0	23.696629	30
GCTTCGC	445	0.0	23.696629	22
>>END_MODULE