FastQCFastQC Report
Fri 10 Feb 2017
ERR1632901.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632901.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141222
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4720.334225545594879No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4130.2924473523955191No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2380.16852898273640085No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACC303.5836498E-430.8333344
ACCGCAA250.005480231329.68
CCGCAAG250.005480231329.69
GTATGTA358.830759E-426.4285721
GTATATA358.830759E-426.4285721
GGTATCA1050.026.428571
TACACTT451.3151989E-424.6666665
TTACACT555.112453E-420.1818184
GAAAAAT500.00700510718.524
AGGGAAA500.00700510718.514
GTATCAA2600.017.7884621
TATAGTA855.3228886E-415.2352942
ATATTAA750.004080747314.7999991
GTATGAA1051.6394616E-414.0952381
CTGAATC800.006262615313.8759
AAATTAT800.006262615313.87527
AACTGAA950.001235633713.6315797
TTAATAA850.00935141413.05882430
TATCAAC3700.013.02
CAACGCA3700.012.55
Produced by FastQC (version 0.11.5)