##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632901.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	141222
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.19896333432468	33.0	33.0	33.0	14.0	33.0
2	30.12549744374106	33.0	33.0	33.0	14.0	33.0
3	30.603581594935633	33.0	33.0	33.0	27.0	33.0
4	30.756291512653835	33.0	33.0	33.0	27.0	33.0
5	30.880054099219667	33.0	33.0	33.0	27.0	33.0
6	32.72431349223209	37.0	33.0	37.0	14.0	37.0
7	33.0357238957103	37.0	33.0	37.0	14.0	37.0
8	33.2237470082565	37.0	33.0	37.0	14.0	37.0
9	33.14146521080285	37.0	33.0	37.0	14.0	37.0
10	33.16449278441036	37.0	33.0	37.0	14.0	37.0
11	33.27374630015154	37.0	33.0	37.0	27.0	37.0
12	33.29353075299883	37.0	33.0	37.0	27.0	37.0
13	33.36036170001841	37.0	33.0	37.0	27.0	37.0
14	33.336356941553014	37.0	33.0	37.0	27.0	37.0
15	33.3719604594185	37.0	33.0	37.0	27.0	37.0
16	33.389394003767116	37.0	33.0	37.0	27.0	37.0
17	33.43226267861948	37.0	33.0	37.0	27.0	37.0
18	33.4021044879693	37.0	33.0	37.0	27.0	37.0
19	33.42564189715483	37.0	33.0	37.0	27.0	37.0
20	33.39811785699112	37.0	33.0	37.0	27.0	37.0
21	33.42076305391511	37.0	33.0	37.0	27.0	37.0
22	33.45728002719123	37.0	33.0	37.0	27.0	37.0
23	33.36812961153361	37.0	33.0	37.0	27.0	37.0
24	33.410913313789635	37.0	33.0	37.0	27.0	37.0
25	33.3587401396383	37.0	33.0	37.0	27.0	37.0
26	33.3127912081687	37.0	33.0	37.0	22.0	37.0
27	33.19736301709366	37.0	33.0	37.0	14.0	37.0
28	33.145288977638046	37.0	33.0	37.0	14.0	37.0
29	33.242667573041025	37.0	33.0	37.0	22.0	37.0
30	33.1640325161802	37.0	33.0	37.0	14.0	37.0
31	33.16202149806687	37.0	33.0	37.0	14.0	37.0
32	33.159373185481016	37.0	33.0	37.0	14.0	37.0
33	33.138292900539575	37.0	33.0	37.0	14.0	37.0
34	33.03335174406254	37.0	33.0	37.0	14.0	37.0
35	33.02471994448457	37.0	33.0	37.0	14.0	37.0
36	32.95251448074663	37.0	33.0	37.0	14.0	37.0
37	33.04255710866579	37.0	33.0	37.0	14.0	37.0
38	32.901028168415685	37.0	33.0	37.0	14.0	37.0
39	32.990313124017504	37.0	33.0	37.0	14.0	37.0
40	32.97815496169152	37.0	33.0	37.0	14.0	37.0
41	33.02653269320644	37.0	33.0	37.0	14.0	37.0
42	32.85857727549532	37.0	33.0	37.0	14.0	37.0
43	32.56996077098469	37.0	33.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	6.0
16	16.0
17	21.0
18	22.0
19	34.0
20	103.0
21	264.0
22	594.0
23	1078.0
24	1777.0
25	2706.0
26	3590.0
27	4763.0
28	5915.0
29	7013.0
30	8603.0
31	10067.0
32	11878.0
33	14365.0
34	18152.0
35	24564.0
36	25687.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.75230488167566	23.09130305476484	12.730311141323588	24.42608092223591
2	16.299160187506196	26.112078854569404	35.28982736400844	22.29893359391596
3	18.723003498038548	28.6917052583875	30.26794692045149	22.31734432312246
4	13.980824517426463	20.688702893316904	37.84821061874212	27.48226197051451
5	15.108127628839698	37.77527580688561	34.849386072991464	12.267210491283228
6	28.64638654034074	41.51902678052994	17.46611717721035	12.368469501918964
7	25.137726416563993	33.65127246463015	23.931115548568922	17.27988557023693
8	23.812153913696164	37.011230544815966	21.053376952599454	18.123238588888416
9	25.60011896163487	15.47492600303069	20.740394556088994	38.18456047924544
10	14.020478395717381	29.542139326733796	34.940731614054464	21.496650663494357
11	33.43388423899959	24.166206398436504	24.70578238518078	17.694126977383124
12	21.869113877441194	26.70901134384161	31.61051394258685	19.811360836130348
13	27.677698942091176	23.068643695741457	26.61483338290068	22.638823979266686
14	21.054793162538417	21.870530087380153	28.591154352721247	28.483522397360183
15	22.85408788998881	30.06684510911898	25.049921400348385	22.029145600543824
16	22.153063970202943	29.463539675121442	25.935052612199232	22.448343742476386
17	21.30192179688717	28.77384543484726	27.533245528317117	22.39098723994845
18	22.015691606123692	28.195323674781548	28.611689396836187	21.177295322258573
19	23.24991856792851	27.593434450722974	28.297290790386764	20.859356190961748
20	23.20814037472915	27.260625115067054	28.351106768067297	21.180127742136495
21	22.262112135502967	28.083443089603605	28.2300208182861	21.424423956607328
22	21.9604594185042	28.13017801758933	28.100437608871136	21.808924955035334
23	21.91868122530484	28.058659415671777	28.40633895568679	21.61632040333659
24	22.11836682669839	28.240642392828313	27.53678605316452	22.104204727308776
25	22.246533826174392	28.244891022645195	28.194615569812072	21.31395958136834
26	22.217501522425685	28.284544900936115	27.95315177521916	21.54480180141904
27	22.362663041169224	27.84976844967498	27.993513758479555	21.79405475067624
28	21.77564402146974	28.168415685941284	28.217983033804934	21.83795725878404
29	22.32654968772571	28.113183498321792	28.03812437155684	21.522142442395662
30	22.440554587812098	27.634504538952854	28.537338375040715	21.387602498194333
31	22.538981178569912	27.643001798586624	28.129469912619847	21.68854711022362
32	21.449207630539153	28.113183498321792	28.30862046989846	22.1289884012406
33	21.517185707609297	28.123096967894522	28.840407301978445	21.519310022517736
34	22.205463737944513	27.843395504949655	28.15212927164323	21.799011485462604
35	21.8981461811899	28.055118890824378	28.317117729532228	21.7296171984535
36	22.424268173514044	27.987848918723714	27.692569146450268	21.89531376131198
37	21.86486524762431	27.938281570860063	28.157086006429594	22.039767175086034
38	22.14952344535554	27.844103609919134	27.905000637294474	22.101372307430854
39	21.652433756780106	28.050870261007493	28.491311552024474	21.80538443018793
40	21.75086034753792	28.206653354293245	28.38651201654133	21.655974281627508
41	21.933551429663932	27.466683661185936	28.39359306623614	22.206171842913992
42	21.820962739516506	27.83914687513277	28.328447409043918	22.011442976306807
43	21.981702567588616	27.436943252467742	28.48635481723811	22.09499936270553
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	3.0
2	5.0
3	26.5
4	48.0
5	48.0
6	65.0
7	82.0
8	86.0
9	90.0
10	136.5
11	183.0
12	183.0
13	260.0
14	337.0
15	618.0
16	899.0
17	1015.5
18	1132.0
19	1132.0
20	1185.5
21	1239.0
22	1507.5
23	1776.0
24	2271.0
25	2766.0
26	2766.0
27	3366.0
28	3966.0
29	4767.0
30	5568.0
31	6324.5
32	7081.0
33	7081.0
34	7725.0
35	8369.0
36	8936.0
37	9503.0
38	10012.5
39	10522.0
40	10522.0
41	10921.0
42	11320.0
43	11297.0
44	11274.0
45	11085.0
46	10896.0
47	10896.0
48	10386.5
49	9877.0
50	9712.5
51	9548.0
52	9145.5
53	8743.0
54	8743.0
55	8028.0
56	7313.0
57	6390.5
58	5468.0
59	4933.0
60	4398.0
61	4398.0
62	3822.0
63	3246.0
64	2657.0
65	2068.0
66	1721.5
67	1375.0
68	1375.0
69	1122.0
70	869.0
71	707.5
72	546.0
73	442.0
74	338.0
75	338.0
76	259.5
77	181.0
78	121.5
79	62.0
80	58.5
81	55.0
82	55.0
83	42.5
84	30.0
85	28.0
86	26.0
87	18.5
88	11.0
89	11.0
90	10.0
91	9.0
92	5.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	141222.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.31993712778636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.12213616467905	70.45557839202571
2	4.855152062434591	7.508001044265698
3	1.4802714095454572	3.433634769543433
4	0.7346384261700701	2.27208787692503
5	0.43072101933514606	1.665166106730477
6	0.2848104278047844	1.321291462272232
7	0.19886722254415928	1.0763480800724325
8	0.13091256270908883	0.809772089432331
9	0.1129245739132559	0.7858188859639509
>10	0.6185868230865836	8.31252659343608
>50	0.02698197774197311	1.4140103797572692
>100	0.003997330035847868	0.9457643195753538
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	472	0.334225545594879	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	413	0.2924473523955191	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	238	0.16852898273640085	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0014162099389613517	0.0	0.0
2	0.0	0.0	0.0014162099389613517	0.0	0.0
3	0.0	0.0	0.0014162099389613517	0.0	0.0
4	0.0	0.0	0.0014162099389613517	0.0	0.0
5	0.0	0.0	0.0014162099389613517	0.0	0.0
6	0.0	0.0	0.003540524847403379	0.0	0.0
7	0.0	0.0	0.004956734786364731	0.0	0.0
8	0.0	0.0	0.006372944725326082	0.0	0.0
9	0.0	0.0	0.006372944725326082	0.0	0.0
10	0.0	0.0	0.007789154664287434	0.0	0.0
11	0.0	0.0	0.016286414298055545	7.081049694806758E-4	0.0
12	0.0	0.0	0.017702624237016896	7.081049694806758E-4	0.0
13	0.0	0.0	0.01841072920649757	7.081049694806758E-4	0.0
14	0.0	0.0	0.021243149084420276	7.081049694806758E-4	0.0
15	0.0	0.0	0.021243149084420276	7.081049694806758E-4	0.0
16	0.0	0.0	0.02195125405390095	7.081049694806758E-4	0.0
17	0.0	0.0	0.02195125405390095	7.081049694806758E-4	0.0
18	0.0	0.0	0.02195125405390095	7.081049694806758E-4	0.0
19	7.081049694806758E-4	0.0	0.022659359023381627	7.081049694806758E-4	0.0
20	7.081049694806758E-4	0.0	0.022659359023381627	7.081049694806758E-4	0.0
21	7.081049694806758E-4	0.0	0.024075568962342978	0.0021243149084420273	0.0
22	7.081049694806758E-4	0.0	0.024075568962342978	0.0021243149084420273	0.0
23	7.081049694806758E-4	0.0	0.024075568962342978	0.0028324198779227034	0.0
24	7.081049694806758E-4	0.0	0.024075568962342978	0.0028324198779227034	0.0
25	7.081049694806758E-4	0.0	0.024075568962342978	0.0028324198779227034	0.0
26	7.081049694806758E-4	0.0	0.024075568962342978	0.0028324198779227034	0.0
27	7.081049694806758E-4	0.0	0.024783673931823652	0.003540524847403379	0.0
28	7.081049694806758E-4	0.0	0.024783673931823652	0.007789154664287434	0.0
29	7.081049694806758E-4	0.0	0.024783673931823652	0.017702624237016896	0.0
30	7.081049694806758E-4	0.0	0.024783673931823652	0.02903230374870771	0.0
31	7.081049694806758E-4	0.0	0.024783673931823652	0.07859965161235502	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACC	30	3.5836498E-4	30.833334	4
ACCGCAA	25	0.0054802313	29.6	8
CCGCAAG	25	0.0054802313	29.6	9
GTATGTA	35	8.830759E-4	26.428572	1
GTATATA	35	8.830759E-4	26.428572	1
GGTATCA	105	0.0	26.42857	1
TACACTT	45	1.3151989E-4	24.666666	5
TTACACT	55	5.112453E-4	20.181818	4
GAAAAAT	50	0.007005107	18.5	24
AGGGAAA	50	0.007005107	18.5	14
GTATCAA	260	0.0	17.788462	1
TATAGTA	85	5.3228886E-4	15.235294	2
ATATTAA	75	0.0040807473	14.799999	1
GTATGAA	105	1.6394616E-4	14.095238	1
CTGAATC	80	0.0062626153	13.875	9
AAATTAT	80	0.0062626153	13.875	27
AACTGAA	95	0.0012356337	13.631579	7
TTAATAA	85	0.009351414	13.058824	30
TATCAAC	370	0.0	13.0	2
CAACGCA	370	0.0	12.5	5
>>END_MODULE