FastQCFastQC Report
Fri 10 Feb 2017
ERR1632900.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632900.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336333
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7240.21526284961630288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5950.17690800486422684No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCCG250.005490884729.69
GGTATCA1700.023.9411751
GCGAACT400.00192836323.12523
ACGGGTC400.00192836323.12515
GGTTAGT400.00192836323.1258
AAAGCGA400.00192836323.12520
CTATTAG502.6965982E-422.21
AACCGAC705.0842336E-621.1428577
TAGAGCC450.003820265620.5555574
ACCGACC656.883589E-519.9230778
CATAATA656.883589E-519.9230772
GTATCAA4650.019.8924731
GAAACCG759.233847E-619.7333345
TAATATA951.667322E-719.4736844
CGAACTA500.007024790618.524
TACCAAC500.007024790618.57
ATAATAC500.007024790618.53
AGGGTGT500.007024790618.56
AAGCGAA500.007024790618.521
TCGTACT500.007024790618.54
Produced by FastQC (version 0.11.5)