FastQCFastQC Report
Fri 10 Feb 2017
ERR1632899.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632899.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48602
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3010.6193160775276737No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2470.508209538702111No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2210.4547137977860993No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC1720.353894901444385No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC1270.2613061190897494No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT1180.24278836261882228No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC1170.24073083412205257No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA1000.20575284967696805No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC970.199580264186659No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA910.1872350932060409No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG870.1790049792189622No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA840.17283239372865314No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.16665980823834411No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA810.16665980823834411No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT700.14402699477387762No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG690.14196946627710794No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA680.13991193778033825No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG680.13991193778033825No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG650.13373935229002923No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT630.12962429529648986No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG630.12962429529648986No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC630.12962429529648986No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC620.12756676679972018No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.12345170980618081No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG600.12345170980618081No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT580.11933665281264146No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG580.11933665281264146No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG550.1131640673223324No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT540.11110653882556273No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC520.10699148183202338No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG520.10699148183202338No Hit
AAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCA510.10493395333525368No Hit
GGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCG500.10287642483848403No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA500.10287642483848403No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC490.10081889634171433No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT490.10081889634171433No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACTG250.00544534429.5999985
GGTATCA706.2664185E-926.428571
TCTAGAA500.006940741618.49999810
GATATGG500.006940741618.4999981
ACGTAGG500.006940741618.49999827
ACTCTAG500.006940741618.4999988
CTCTAGA500.006940741618.4999989
ATGGACT500.006940741618.4999984
TGGACTC500.006940741618.4999985
ATATGGA500.006940741618.4999982
TCCCTAG500.006940741618.49999833
TATGGAC500.006940741618.4999983
GTATCAA1451.4188117E-1017.8620681
GGATTGC700.002544916515.85714219
TACGGGA1050.002581312.3333332
AACGCAG2451.3897079E-912.0816336
TCAACGC2451.3897079E-912.0816334
ATCAACG2451.3897079E-912.0816333
CAACGCA2451.3897079E-912.0816335
GAGTACT1451.8663844E-411.48275856
Produced by FastQC (version 0.11.5)