##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632899.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	48602
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.109110736183695	33.0	27.0	33.0	14.0	33.0
2	29.357001769474508	33.0	27.0	33.0	14.0	33.0
3	29.875498950660468	33.0	27.0	33.0	14.0	33.0
4	29.973869388091025	33.0	33.0	33.0	14.0	33.0
5	30.153594502283855	33.0	33.0	33.0	14.0	33.0
6	30.114501460845233	33.0	27.0	37.0	14.0	37.0
7	30.66561046870499	37.0	27.0	37.0	14.0	37.0
8	30.814308053166535	37.0	27.0	37.0	14.0	37.0
9	30.20019752273569	33.0	27.0	37.0	14.0	37.0
10	30.151372371507346	33.0	27.0	37.0	14.0	37.0
11	30.434961524217112	33.0	27.0	37.0	14.0	37.0
12	30.620324266491092	37.0	27.0	37.0	14.0	37.0
13	30.73519608246574	37.0	27.0	37.0	14.0	37.0
14	30.581951360026338	37.0	27.0	37.0	14.0	37.0
15	30.89566272992881	37.0	27.0	37.0	14.0	37.0
16	30.94354141804864	37.0	27.0	37.0	14.0	37.0
17	31.070449775729394	37.0	27.0	37.0	14.0	37.0
18	31.01794164849183	37.0	27.0	37.0	14.0	37.0
19	30.986893543475578	37.0	27.0	37.0	14.0	37.0
20	31.006686967614502	37.0	27.0	37.0	14.0	37.0
21	31.073042261635322	37.0	27.0	37.0	14.0	37.0
22	31.06154067733838	37.0	27.0	37.0	14.0	37.0
23	31.000864161968643	37.0	27.0	37.0	14.0	37.0
24	31.01211884284597	37.0	27.0	37.0	14.0	37.0
25	30.960248549442408	37.0	27.0	37.0	14.0	37.0
26	30.866939632113905	37.0	27.0	37.0	14.0	37.0
27	30.82276449528826	37.0	27.0	37.0	14.0	37.0
28	30.719558865890292	37.0	27.0	37.0	14.0	37.0
29	30.781552199497963	37.0	27.0	37.0	14.0	37.0
30	30.851178963828648	37.0	27.0	37.0	14.0	37.0
31	30.705773424961937	37.0	27.0	37.0	14.0	37.0
32	30.675507180774453	37.0	27.0	37.0	14.0	37.0
33	30.624789103329082	37.0	27.0	37.0	14.0	37.0
34	30.49633759927575	37.0	27.0	37.0	14.0	37.0
35	30.581066622772724	37.0	27.0	37.0	14.0	37.0
36	30.569606189045718	37.0	27.0	37.0	14.0	37.0
37	30.617958108719804	37.0	27.0	37.0	14.0	37.0
38	30.457738364676352	37.0	27.0	37.0	14.0	37.0
39	30.5166248302539	37.0	27.0	37.0	14.0	37.0
40	30.438726801366197	37.0	27.0	37.0	14.0	37.0
41	30.542467388173325	37.0	27.0	37.0	14.0	37.0
42	30.294308876177936	37.0	27.0	37.0	14.0	37.0
43	29.986646640055966	33.0	27.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	8.0
16	13.0
17	16.0
18	52.0
19	116.0
20	274.0
21	494.0
22	832.0
23	1260.0
24	1756.0
25	2330.0
26	2890.0
27	3237.0
28	3504.0
29	3839.0
30	4061.0
31	4136.0
32	4039.0
33	4084.0
34	3738.0
35	3633.0
36	4289.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.907658121064976	24.44961112711411	10.067486934694045	21.575243817126868
2	18.60417266779145	27.634665240113577	31.918439570388053	21.842722521706925
3	20.357186947039217	29.443232788774125	28.747788156865973	21.451792107320685
4	15.363565285379202	24.682111847249082	34.70844821200774	25.245874655363977
5	17.614501460845233	37.74536027323979	33.16735936792725	11.472778897987737
6	28.05645858195136	41.11353442245175	16.836755689066294	13.993251306530595
7	26.90424262376034	33.5233117978684	22.894119583556233	16.678325994815026
8	24.282951318875767	35.84008888523106	20.32015143409736	19.55680836179581
9	26.342537344142215	15.295666844985803	20.73371466194807	37.62808114892391
10	16.902596600962923	28.381548084440972	33.13443891197893	21.581416402617176
11	34.05209662153821	23.94963170239908	23.597794329451464	18.40047734661125
12	23.02580140734949	28.13258713633184	28.766305913336897	20.07530554298177
13	28.48853956627299	23.332373153368174	25.496893131969877	22.682194148388955
14	22.33652936093165	21.90033331961648	28.060573638944895	27.702563680506977
15	25.37755647915724	28.330109872021726	25.474260318505408	20.818073330315627
16	22.21513517962224	28.59141599111148	26.303444302703593	22.890004526562695
17	21.941483889551872	27.994732727048273	26.610016048722272	23.453767334677586
18	21.848895107197237	27.22727459775318	28.599646105098554	22.32418418995103
19	25.523641002427883	25.828155219949796	28.521460022221305	20.126743755401012
20	24.51339451051397	25.657380354717912	29.26422780955516	20.564997325212957
21	22.817991029175754	27.926834286654874	28.930908193078476	20.3242664910909
22	22.377679930867043	27.819842804822848	28.08732150940291	21.715155754907205
23	22.274803506028558	26.87132216781202	30.539895477552363	20.313978848607054
24	22.001152215958193	28.69017735895642	26.50096703839348	22.807703386691905
25	22.534052096621537	28.07291880992552	28.716925229414425	20.676103864038517
26	22.673964034401877	27.953582157112876	29.159293856219907	20.21315995226534
27	23.159540759639523	26.84251676885725	27.902143944693634	22.095798526809595
28	21.892103205629397	27.59351467017818	29.82181803217975	20.692564092012674
29	23.834410106579977	27.509156001810624	27.449487675404306	21.206946216205093
30	22.645158635447103	26.579153121270732	29.445290317270896	21.330397926011273
31	23.122505246697667	27.23756224023703	28.344512571499113	21.29541994156619
32	21.18842845973417	27.86922348874532	28.37126044195712	22.571087609563392
33	21.0978972058763	27.085305131476073	29.760092177276654	22.056705485370973
34	22.20279000864162	27.027694333566522	29.161351384716678	21.608164273075182
35	22.112258754783753	28.033825768486892	28.599646105098554	21.254269371630798
36	23.04637669231719	28.288959302086337	26.959795893173123	21.704868112423355
37	22.665733920414798	27.212871898275793	28.268384017118635	21.853010164190774
38	22.124603925764372	27.47829307435908	28.105839265873833	22.291263734002715
39	22.151351796222375	27.898028887700093	28.727212871898278	21.22340644417925
40	22.383852516357354	27.838360561293772	29.095510472820045	20.682276449528825
41	22.379737459363813	26.721122587547836	27.963869799596726	22.935270153491626
42	21.585531459610717	28.134644664828606	28.336282457512034	21.94354141804864
43	22.828278671659604	26.877494753302333	27.54001892926217	22.754207645775896
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	5.5
2	4.0
3	12.0
4	20.0
5	20.0
6	33.5
7	47.0
8	52.5
9	58.0
10	83.5
11	109.0
12	109.0
13	174.5
14	240.0
15	390.5
16	541.0
17	558.0
18	575.0
19	575.0
20	508.5
21	442.0
22	326.5
23	211.0
24	266.0
25	321.0
26	321.0
27	417.5
28	514.0
29	668.5
30	823.0
31	1088.0
32	1353.0
33	1353.0
34	1943.5
35	2534.0
36	2909.0
37	3284.0
38	3582.0
39	3880.0
40	3880.0
41	4244.5
42	4609.0
43	4740.5
44	4872.0
45	4720.0
46	4568.0
47	4568.0
48	4496.5
49	4425.0
50	4252.0
51	4079.0
52	3684.5
53	3290.0
54	3290.0
55	3061.0
56	2832.0
57	2283.0
58	1734.0
59	1537.0
60	1340.0
61	1340.0
62	1137.0
63	934.0
64	678.5
65	423.0
66	336.5
67	250.0
68	250.0
69	191.5
70	133.0
71	102.0
72	71.0
73	57.5
74	44.0
75	44.0
76	28.5
77	13.0
78	11.0
79	9.0
80	7.5
81	6.0
82	6.0
83	4.5
84	3.0
85	2.0
86	1.0
87	1.5
88	2.0
89	2.0
90	1.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	48602.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.69326365170157
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.03556100551809	44.04757005884532
2	7.3038013488657265	7.843298629686021
3	3.2878602084610664	5.296078350685157
4	1.7282342121397916	3.7117814081725027
5	1.1649294911097487	3.1274433150899137
6	0.7664009809932557	2.4690341961236166
7	0.5633047210300429	2.117196823176001
8	0.4636725935009197	1.9916875848730506
9	0.3793684855916616	1.833257890621785
>10	2.184242795830779	21.35920332496605
>50	0.09580012262415696	3.522488786469693
>100	0.02682403433476395	2.6809596312908934
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	301	0.6193160775276737	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	247	0.508209538702111	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	221	0.4547137977860993	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	172	0.353894901444385	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	127	0.2613061190897494	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	118	0.24278836261882228	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	117	0.24073083412205257	No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA	100	0.20575284967696805	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	97	0.199580264186659	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	91	0.1872350932060409	No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG	87	0.1790049792189622	No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA	84	0.17283239372865314	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81	0.16665980823834411	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	81	0.16665980823834411	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	70	0.14402699477387762	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	69	0.14196946627710794	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	68	0.13991193778033825	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	68	0.13991193778033825	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	65	0.13373935229002923	No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT	63	0.12962429529648986	No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG	63	0.12962429529648986	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	63	0.12962429529648986	No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC	62	0.12756676679972018	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60	0.12345170980618081	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	60	0.12345170980618081	No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT	58	0.11933665281264146	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	58	0.11933665281264146	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	55	0.1131640673223324	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	54	0.11110653882556273	No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC	52	0.10699148183202338	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	52	0.10699148183202338	No Hit
AAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCA	51	0.10493395333525368	No Hit
GGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCG	50	0.10287642483848403	No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA	50	0.10287642483848403	No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC	49	0.10081889634171433	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	49	0.10081889634171433	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0020575284967696804	0.0	0.0
2	0.0	0.0	0.0020575284967696804	0.0	0.0
3	0.0	0.0	0.0020575284967696804	0.0	0.0
4	0.0	0.0	0.004115056993539361	0.0	0.0
5	0.0	0.0	0.008230113987078722	0.0	0.0
6	0.0	0.0	0.008230113987078722	0.0	0.0
7	0.0	0.0	0.010287642483848402	0.0	0.0
8	0.0	0.0	0.010287642483848402	0.0	0.0
9	0.0	0.0	0.014402699477387761	0.0	0.0
10	0.0	0.0	0.014402699477387761	0.0	0.0
11	0.0	0.0	0.020575284967696804	0.0	0.0
12	0.0	0.0	0.022632813464466484	0.0	0.0
13	0.0	0.0	0.022632813464466484	0.0	0.0
14	0.0	0.0	0.024690341961236165	0.0	0.0
15	0.0	0.0	0.024690341961236165	0.0	0.0
16	0.0	0.0	0.032920455948314886	0.0	0.0
17	0.0	0.0	0.032920455948314886	0.0	0.0
18	0.0	0.0	0.032920455948314886	0.0	0.0
19	0.0	0.0	0.032920455948314886	0.0	0.0
20	0.0	0.0	0.032920455948314886	0.0	0.0
21	0.0	0.0	0.032920455948314886	0.0	0.0
22	0.0	0.0	0.032920455948314886	0.0	0.0
23	0.0	0.0	0.032920455948314886	0.0020575284967696804	0.0
24	0.0	0.0	0.032920455948314886	0.004115056993539361	0.0
25	0.0	0.0	0.032920455948314886	0.004115056993539361	0.0
26	0.0	0.0	0.032920455948314886	0.004115056993539361	0.0
27	0.0	0.0	0.032920455948314886	0.004115056993539361	0.0
28	0.0	0.0	0.032920455948314886	0.010287642483848402	0.0
29	0.0	0.0	0.032920455948314886	0.024690341961236165	0.0
30	0.0	0.0	0.03497798444508456	0.03497798444508456	0.0
31	0.0	0.0	0.03497798444508456	0.08847372536109625	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAAACTG	25	0.005445344	29.599998	5
GGTATCA	70	6.2664185E-9	26.42857	1
TCTAGAA	50	0.0069407416	18.499998	10
GATATGG	50	0.0069407416	18.499998	1
ACGTAGG	50	0.0069407416	18.499998	27
ACTCTAG	50	0.0069407416	18.499998	8
CTCTAGA	50	0.0069407416	18.499998	9
ATGGACT	50	0.0069407416	18.499998	4
TGGACTC	50	0.0069407416	18.499998	5
ATATGGA	50	0.0069407416	18.499998	2
TCCCTAG	50	0.0069407416	18.499998	33
TATGGAC	50	0.0069407416	18.499998	3
GTATCAA	145	1.4188117E-10	17.862068	1
GGATTGC	70	0.0025449165	15.857142	19
TACGGGA	105	0.0025813	12.333333	2
AACGCAG	245	1.3897079E-9	12.081633	6
TCAACGC	245	1.3897079E-9	12.081633	4
ATCAACG	245	1.3897079E-9	12.081633	3
CAACGCA	245	1.3897079E-9	12.081633	5
GAGTACT	145	1.8663844E-4	11.4827585	6
>>END_MODULE