FastQCFastQC Report
Fri 10 Feb 2017
ERR1632898.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632898.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332037
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7790.23461240765336394No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5510.16594536151091596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGC509.065687E-625.89999810
CCTTAGG451.3204446E-424.6666682
GGTATCA2550.023.9411751
GACAGCG551.8973798E-523.54545437
GTATTAG1103.6379788E-1223.5454541
GTCTTAT400.00192831123.1250021
CCGTATG502.6964978E-422.1999999
TATTAGC502.6964978E-422.1999992
GTATCAA5850.022.1367531
ACCGCCT603.7160207E-521.58333412
TAATTGA450.003820164120.5555574
TATTAGA759.233268E-619.7333322
TTAGAGG1254.0927262E-1019.244
TAACGGC609.217966E-418.536
TTAACGG609.217966E-418.535
CACCGCC801.6110342E-518.511
GAGGCAC701.21588644E-418.57
CAACGCA7500.017.5133345
GTGTAGG852.713683E-517.4117641
GGCTTAT752.0616932E-417.2666661
Produced by FastQC (version 0.11.5)