FastQCFastQC Report
Fri 10 Feb 2017
ERR1632897.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632897.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281055
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT8440.30029709487466866No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC7350.26151465015744246No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG7010.24941737382362883No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6980.24834996708829232No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC6960.247638362598068No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC6140.21846257849887032No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT5800.20636530216505666No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA5360.1907100033801213No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA5030.17896852929141985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4920.175054704595186No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT4680.166515450712494No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC4660.16580384622226968No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA4460.15868780132002633No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC4380.155841383359129No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG4360.15512977886890467No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA4250.15121595417267084No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA4150.14765793172154917No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC4010.14267670028997884No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG3800.13520485314262334No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT3790.13484905089751115No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG3770.13413744640728684No Hit
CATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCT3560.12666559925993132No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA3480.123819181299034No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG3410.12132856558324882No Hit
ATGTTATTCCTGTTCCTCTTGGTATTCATCAACCACTGCACAA3380.12026115884791232No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG3290.11705893864190284No Hit
TGTTATTCCTGTTCCTCTTGGTATTCATCAACCACTGCACAAA3280.11670313639679065No Hit
CTGTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGC3170.11278931170055682No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT3150.1120777072103325No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC3040.10816388251409866No Hit
ATGCAAAGCAATGTGGCCTCAGAATACACCTCTTAAATTTACA3000.10674067353365No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC3000.10674067353365No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA2840.10104783761185535No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAACG200.001839570937.034
ATAACGA200.001839570937.035
TAACGAC250.00548936729.636
AACGACA250.00548936729.637
GTATACT250.00548936729.61
CCCATAG502.7084207E-729.61
GGTATCA1400.026.428571
CCGTTAA358.852664E-426.4285716
TAACGGC601.3314093E-624.66666636
AACGGCC601.3314093E-624.66666637
GTATCAA3750.023.1866651
GTCGCCC400.001927578223.12500237
CGCGGCC400.001927578223.12500211
GGCCGTT400.001927578223.12500214
ATTAATC400.001927578223.1250023
CCATAGT652.6708894E-622.7692282
CGTTAAA502.6950694E-422.217
TCGCCAT1351.8189894E-1220.55555513
CCTTATA450.003818727820.5555552
TGATAAC450.003818727820.55555533
Produced by FastQC (version 0.11.5)